Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,577	T→C	intergenic (+36/‑4)	rhsD → / → ybbC	protein RhsD/PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,577	0	T	C	100.0%	193.5 / NA	56	intergenic (+36/‑4)	rhsD/ybbC	protein RhsD/PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

CTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527441‑527713                                                                                                                                         |                                                                                                                                         cTGATGGACGATTTGCGGCGCTGAAGAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCa                                                                                                                                       <  1:333867/140‑1 (MQ=255) cTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCa                                                                                                                                       <  1:585131/140‑1 (MQ=255)         cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTCTTACCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata                                                                                                                               <  2:559068/140‑1 (MQ=255)           aTTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAAtata                                                                                                                             >  1:576826/1‑140 (MQ=255)           aTTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTGTATTAGTAGGGACTGTAAGGAGACTCAATGAAAtata                                                                                                                             >  1:231683/1‑140 (MQ=255)                  gcgcTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAat                                                                                                                      <  1:215469/140‑1 (MQ=255)                     cTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTAGGGCCTATAAGGAGACGCAATAAAATATAGTTCAATAtt                                                                                                                   >  2:342488/1‑140 (MQ=255)                               aaGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCtt                                                                                                         >  1:705756/1‑140 (MQ=255)                                aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  2:563624/1‑140 (MQ=255)                                 ggAGAATATATTTTTATTAGAATTAGTGTCCTGGGTTTGAGTTCAGTACGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTc                                                                                                       <  2:231683/140‑1 (MQ=255)                                 ggAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTc                                                                                                       <  2:576826/140‑1 (MQ=255)                                  gagaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCa                                                                                                      >  2:530997/1‑140 (MQ=255)                                     aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  2:46428/1‑140 (MQ=255)                                       tatatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttttt                                                                                                 >  2:646958/1‑140 (MQ=255)                                         tatTTTTATCAGAAATAGTGTCCCGGGATTAAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTta                                                                                               <  1:543304/140‑1 (MQ=255)                                           tttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTtata                                                                                             <  1:646958/140‑1 (MQ=255)                                            ttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATAc                                                                                            >  2:828071/1‑140 (MQ=255)                                            ttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATAc                                                                                            >  2:804944/1‑140 (MQ=255)                                             tttATCAGAAATAGTGTCCCGGGATTGAGTTCATTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAAAATAGTTCGATACTTTCGATGCTTTCATTTTTTATACt                                                                                           >  1:308623/1‑140 (MQ=255)                                                aTCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAtt                                                                                        <  1:571873/140‑1 (MQ=255)                                                 tCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAttt                                                                                       >  2:635983/1‑140 (MQ=255)                                                    gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGcc                                                                                    <  1:530997/140‑1 (MQ=255)                                                    gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGcc                                                                                    <  2:6591/140‑1 (MQ=255)                                                    gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGcc                                                                                    <  1:709149/140‑1 (MQ=255)                                                    gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGcc                                                                                    <  1:635983/140‑1 (MQ=255)                                                             tCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTACTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGCTCAATATTCTCGCTGCGTTCATTTTTTATATTATTTGCCTGTAATgag                                                                           >  2:321698/1‑140 (MQ=255)                                                                  ggATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGc                                                                      <  2:489666/140‑1 (MQ=255)                                                                     ttGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTACATTTTTTATAGTATTTGCCTTTAATGAGACAGCTGt                                                                   >  1:414456/1‑140 (MQ=255)                                                                        aGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa                                                                <  2:705756/140‑1 (MQ=255)                                                                                    aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga                                                    >  2:689051/1‑140 (MQ=255)                                                                                         gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  2:780035/1‑140 (MQ=255)                                                                                         gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  2:461962/1‑140 (MQ=255)                                                                                           aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTTTAATGAGCCAGCTGCTTACGGCTCTGATGAAAACatt                                             >  1:411201/1‑140 (MQ=255)                                                                                           aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt                                             >  2:204030/1‑140 (MQ=255)                                                                                             cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTGATGAGACAGCTCTTCACGGTTGTGATGAAAACattat                                           >  1:419922/1‑140 (MQ=255)                                                                                               tGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTAttt                                         >  2:201874/1‑140 (MQ=255)                                                                                                  ttcaTTTTAGTCCTTTTATTAGCGGGGCCTATAAGGCGACTCAATGAAATATAGCTCAATATTTTCGATCCTTTCATTTTCTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTAttttga                                      >  2:81648/4‑138 (MQ=255)                                                                                                   tAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTAt                                     <  1:461962/140‑1 (MQ=255)                                                                                                    aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATg                                    <  1:472640/140‑1 (MQ=255)                                                                                                          aGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGGTTACGGTGCTGTTGGAAACATTATTTTTATGAGGTAt                              >  1:315719/1‑140 (MQ=255)                                                                                                                tttATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTACTGATGAAAACAGTATTTTTATGAGGTATGTGGaa                        >  2:353893/1‑140 (MQ=255)                                                                                                                      aGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTa                  <  1:676197/140‑1 (MQ=255)                                                                                                                          gggCCTATTAGGAGACCAAATGTAATATAGTTCAATATTCTCGATGCTCTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:419922/140‑1 (MQ=255)                                                                                                                          gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:46428/140‑1 (MQ=255)                                                                                                                          gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:137595/140‑1 (MQ=255)                                                                                                                          gggCCTATAAGGAGACGTAATGAAATATAGTGCTACATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:411201/140‑1 (MQ=255)                                                                                                                           ggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt             >  2:826230/1‑140 (MQ=255)                                                                                                                           ggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATCTTTATGAGGTATGTGGAAAAATTACAttt             >  1:349523/1‑140 (MQ=255)                                                                                                                            gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTa            <  1:201874/140‑1 (MQ=255)                                                                                                                             ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:847672/137‑1 (MQ=255)                                                                                                                             ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              >  1:847672/1‑137 (MQ=255)                                                                                                                             ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg           >  1:731051/1‑140 (MQ=255)                                                                                                                             ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg           >  2:830774/1‑140 (MQ=255)                                                                                                                              cTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:206801/136‑1 (MQ=255)                                                                                                                              cTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              >  1:206801/1‑136 (MQ=255)                                                                                                                                      agaCTCAATGAAATATAGTTCATTAGTTTGGATGCTTGCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  1:348610/140‑1 (MQ=255)                                                                                                                                      agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:731051/140‑1 (MQ=255)                                                                                                                                      agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:591800/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527441‑527713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑