Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,625	A→G	R80R (CGT→CGC)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,625	0	A	G	100.0%	210.5 / NA	59	R80R (CGT→CGC)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

TCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGT  >  NC_000913/80501‑80759                                                                                                                             |                                                                                                                                       tCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCg                                                                                                                         <  1:669448/140‑1 (MQ=255) tCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCg                                                                                                                         <  1:552514/140‑1 (MQ=255)  ccccGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGc                                                                                                                        <  1:761611/140‑1 (MQ=255)   cccGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCa                                                                                                                       >  1:174602/1‑140 (MQ=255)   cccGGCAAGGTGACGACCTGTTCCGACCCGATTACGTTGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCa                                                                                                                       <  1:341168/140‑1 (MQ=255)     cGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAgg                                                                                                                     >  1:605137/1‑140 (MQ=255)     cGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAgg                                                                                                                     >  2:511949/1‑140 (MQ=255)       gCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCa                                                                                                                   >  2:490731/1‑140 (MQ=255)       gCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCa                                                                                                                   <  1:466247/140‑1 (MQ=255)       gCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCa                                                                                                                   <  2:174602/140‑1 (MQ=255)           ggTGACGCCCTGTTCTGGCCCCGTTCCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTaa                                                                                                               <  1:381947/140‑1 (MQ=255)           ggTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTaa                                                                                                               <  2:531564/140‑1 (MQ=255)           ggTGACGCCCTGTTCAGGCCCCCTTTCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTaa                                                                                                               <  1:315828/140‑1 (MQ=255)            gTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTACTGCATCGGGATGCGCGCCATGTCACCGCAGGCATTAAt                                                                                                              >  2:381947/1‑140 (MQ=255)                     tGTTCCGGCCCCATTTCGTGGATGAACCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGt                                                                                                     <  1:237987/140‑1 (MQ=255)                     tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGt                                                                                                     <  1:490731/140‑1 (MQ=255)                     tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGt                                                                                                     <  1:615526/140‑1 (MQ=255)                     tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCCTTGGt                                                                                                     <  2:612229/140‑1 (MQ=255)                      gTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                                                                                                    >  2:465826/1‑140 (MQ=255)                            gCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                                                                                                    >  2:133279/1‑134 (MQ=255)                            gCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                                                                                                    <  1:133279/134‑1 (MQ=255)                            gCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTa                                                                                              >  2:715254/1‑140 (MQ=255)                               ccATTGCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTagag                                                                                           <  2:605137/140‑1 (MQ=255)                                 aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCt                                                                                                          >  2:158783/1‑123 (MQ=255)                                 aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCt                                                                                                          <  1:158783/123‑1 (MQ=255)                                 aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAc                                                                                         >  2:834812/1‑140 (MQ=255)                                 aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCACGCACTAATGTCTTTGGTCTGGGTAGAGAc                                                                                         >  1:318277/1‑140 (MQ=255)                                     cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg                                                                                     <  2:771070/140‑1 (MQ=255)                                     cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg                                                                                     <  1:656995/140‑1 (MQ=255)                                     cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg                                                                                     <  1:511949/140‑1 (MQ=255)                                     cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAATTCGACGCAAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTGGAGACGTtg                                                                                     <  2:311593/140‑1 (MQ=255)                                           cGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATcc                                                                               >  2:769199/1‑140 (MQ=255)                                                    gATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCg                                                                      >  2:847595/1‑140 (MQ=255)                                                        cGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAgg                                                                  >  2:776439/1‑140 (MQ=255)                                                        cGGGTGATTCAGGTCATACAGTTCGACACCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAgg                                                                  >  1:793861/1‑140 (MQ=255)                                                          ggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGtt                                                                >  2:77138/1‑140 (MQ=255)                                                          ggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGtt                                                                >  1:215907/1‑140 (MQ=255)                                                              aTTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGc                                                            >  1:624942/1‑140 (MQ=255)                                                               ttCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTGTTTGGTCTGGGCAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGcc                                                           >  2:797960/1‑140 (MQ=255)                                                                tCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGccc                                                          <  2:464893/140‑1 (MQ=255)                                                                tCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGccc                                                          <  2:215907/140‑1 (MQ=255)                                                                gagacagaTACAGTTCGACGCCAAACTCTTTGCAGTTATTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGccc                                                          <  1:282319/133‑1 (MQ=255)                                                                  aGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCgg                                                        <  1:77138/140‑1 (MQ=255)                                                                         aCAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGCGTAGAGACGTTGTGATGCATGGTAGGGAAGGTTTTGCCCGGCTGACGt                                                 >  2:369631/1‑140 (MQ=255)                                                                         aCAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGCGTAGAGACATTGTGATACATGGTAGCGAAGATTTTGCCCGGCTGACGt                                                 >  2:313832/1‑140 (MQ=255)                                                                          cAGTTCGACGCCAAATTCTTTGAAGCTTTGGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTa                                                <  1:313832/140‑1 (MQ=255)                                                                              tCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCgg                                            >  2:583160/1‑140 (MQ=255)                                                                              tCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCgg                                            >  1:200797/1‑140 (MQ=255)                                                                                  cGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACTTTGTGATCCATGATAGGGAAGGTTTAGCCCGGCTGACGTAcctgtctc                                        >  2:282319/1‑134 (MQ=255)                                                                                             ttGCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcgcg                             <  1:834812/140‑1 (MQ=255)                                                                                               gCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcgcg                             >  2:725346/1‑138 (MQ=255)                                                                                               gCAGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcgcg                             <  1:725346/138‑1 (MQ=255)                                                                                                 aGTTTTTGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGa                         <  2:200797/140‑1 (MQ=255)                                                                                                       tGATCAGTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCg                   <  2:793861/140‑1 (MQ=255)                                                                                                             gTTCCTGCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGGAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACATACCGGGGGACCGTTGGCGCGAAGACCATCGAACGcc             >  1:307998/1‑140 (MQ=255)                                                                                                                   gCATCTGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGt       <  1:769199/140‑1 (MQ=255)                                                                                                                        tGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGt  >  2:643999/1‑140 (MQ=255)                                                                                                                        tGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGt  >  2:654834/1‑140 (MQ=255)                                                                                                                        tGGATGCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGt  >  2:218500/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                       TCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGT  >  NC_000913/80501‑80759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑