Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	530,394	T→C	Y278C (TAT→TGT)	selU ←	tRNA 2‑selenouridine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	530,394	0	T	C	100.0%	77.0 / NA	24	Y278C (TAT→TGT)	selU	tRNA 2‑selenouridine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/16);  total (8/16)

GGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCA  >  NC_000913/530258‑530526                                                                                                                                         |                                                                                                                                     ggccagATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACt                                                                                                                                   <  1:863123/140‑1 (MQ=255)       aTGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGcc                                                                                                                             >  1:697528/1‑140 (MQ=255)           ccGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGcc                                                                                                                         <  1:347435/140‑1 (MQ=255)                  tgctgcCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTtcg                                                                                                                  <  2:697528/140‑1 (MQ=255)                   gctgcCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTtcgt                                                                                                                 <  2:77006/140‑1 (MQ=255)                   gctgcCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTtcgt                                                                                                                 <  2:125622/140‑1 (MQ=255)                              tgAGTTGCGTTGTCACTGCTGCCTCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTAcgcg                                                                                                      <  1:286120/140‑1 (MQ=255)                                                 tgcATCCAGCCTTGCAGCCAGTTCGTTATAGGGCTTTAGGCCCAGCCGCCGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCa                                                                                   <  2:329413/140‑1 (MQ=255)                                                 tgcATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCa                                                                                   <  2:746555/140‑1 (MQ=255)                                                      ccAGCCTTGCAGCCACTTCGTTGTATCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGc                                                                              <  1:405124/140‑1 (MQ=255)                                                        aGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGAGGTTAAAATCATGATGCATACGCaa                                                                            >  1:329413/1‑140 (MQ=255)                                                               cAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATAc                                                                     >  1:462393/1‑140 (MQ=255)                                                                    aGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTc                                                                >  2:814284/1‑140 (MQ=255)                                                                          ttATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAg                                                          <  2:462393/140‑1 (MQ=255)                                                                                cgcTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTc                                                    >  2:29485/1‑140 (MQ=255)                                                                                cgcTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTc                                                    >  2:508411/1‑140 (MQ=255)                                                                                          ccAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGAtc                                          <  1:814284/140‑1 (MQ=255)                                                                                               cGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCaaa                                     <  1:29485/140‑1 (MQ=255)                                                                                                 aCGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACg                                   <  2:52986/140‑1 (MQ=255)                                                                                                 aCGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACg                                   <  1:508411/140‑1 (MQ=255)                                                                                                                          tGCAGGTATTCGCAACACTCCTTCCAGCCCTCTTCATCGTCGTACGCGAGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATcgccgc          <  2:228636/140‑1 (MQ=255)                                                                                                                                  ttCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCCTCTACCACCGCAATcgccgc          <  2:647499/132‑1 (MQ=255)                                                                                                                                  ttCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCCTCTACCACCGCAATcgccgc          >  1:647499/1‑132 (MQ=255)                                                                                                                                  ttCGCAACACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATATTCAACAACCGCAGTCGCCGCCTGAGTCa  >  1:228636/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTCA  >  NC_000913/530258‑530526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑