Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	595,667	A→G	D23G (GAT→GGT)	cusC →	copper/silver export system outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	595,667	0	A	G	100.0%	139.0 / NA	39	D23G (GAT→GGT)	cusC	copper/silver export system outer membrane channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

TCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGATTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGAAGACGCTGATTAG  >  NC_000913/595535‑595802                                                                                                                                     |                                                                                                                                        tCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAg                                                                                                                                  >  1:812680/1‑140 (MQ=255)     cGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTc                                                                                                                              <  1:504005/140‑1 (MQ=255)                  ccTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGt                                                                                                                 >  2:853787/1‑140 (MQ=255)                      gCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCg                                                                                                             <  1:748246/140‑1 (MQ=255)                          gTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCgcagc                                                                                                         <  1:429992/140‑1 (MQ=255)                                    cggcATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTctcact                                                                                               >  1:6034/1‑140 (MQ=255)                                     ggcATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTctcactc                                                                                              >  2:148058/1‑140 (MQ=255)                                            aaTCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGa                                                                                       <  1:853787/140‑1 (MQ=255)                                            aaTCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGa                                                                                       <  1:730418/140‑1 (MQ=255)                                                     aaaTTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTgg                                                                              >  2:426935/1‑140 (MQ=255)                                                           tGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACg                                                                        <  2:784584/140‑1 (MQ=255)                                                           tGAGCCTATGTCTCCTTGTAAAATTGTGCTATTTTGTTTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACg                                                                        <  1:333473/140‑1 (MQ=255)                                                            gAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGc                                                                                  >  2:604343/1‑129 (MQ=255)                                                            gAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGc                                                                                  <  1:604343/129‑1 (MQ=255)                                                            gAGCCTATGTCTCCTTGTAAACGTCTGCCGTTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACgc                                                                       <  1:355121/140‑1 (MQ=255)                                                               ccTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTaa                                                                          <  1:718713/134‑1 (MQ=255)                                                               ccTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTaa                                                                          >  2:718713/1‑134 (MQ=255)                                                               ccTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACgcagc                                                                    <  2:192773/140‑1 (MQ=255)                                                                   tGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGc                                                                                  <  1:561325/122‑1 (MQ=255)                                                                   tGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGc                                                                                  >  2:561325/1‑122 (MQ=255)                                                                   tGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGAt                                                                >  2:463805/1‑140 (MQ=255)                                                                         cTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTAt                                                          <  2:69664/140‑1 (MQ=255)                                                                         cTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTAt                                                          >  2:679931/1‑140 (MQ=255)                                                                         cTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTAt                                                          >  1:705485/1‑140 (MQ=255)                                                                                   tCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATaa                                                              <  1:796780/126‑1 (MQ=255)                                                                                   tCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATaa                                                              >  2:796780/1‑126 (MQ=255)                                                                                          ttttGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGgcg                                         <  2:812680/140‑1 (MQ=255)                                                                                          ttttGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGgcg                                         >  2:549331/1‑140 (MQ=255)                                                                                             tgtgtgGCCCTTGCGCTCACCGCTTGTTCAAAGGCCCCGGGTTATCAGGGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCAc                                      <  2:424324/140‑1 (MQ=255)                                                                                              gtgtgGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAAGGCGGGCTGGCGCAcc                                     >  1:424324/1‑140 (MQ=255)                                                                                              gtgtgGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCAc                                      <  2:486463/139‑1 (MQ=255)                                                                                              gtgtgGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCAc                                      >  1:486463/1‑139 (MQ=255)                                                                                                         gcgcTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAGCGCAGCAGCTAAATATGAGAACGCGTGCTGGCGCACCTTTTTTGTTGa                          >  2:303128/1‑140 (MQ=255)                                                                                                         gcgcTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGa                          >  1:675416/1‑140 (MQ=255)                                                                                                            cTAACCGGTTGTTCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATaa                       <  1:679931/140‑1 (MQ=255)                                                                                                                    ttgttCACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGa               >  2:103989/1‑140 (MQ=255)                                                                                                                         cACTGGCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGAAGACg          <  1:707586/140‑1 (MQ=255)                                                                                                                              gCACCGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGAAGACGCTGAt     >  1:805816/1‑140 (MQ=255)                                                                                                                                 ccGGGTTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGAAGACGCTGATTAg  <  2:675416/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCGGATTATCAGCGTCCGGCAATGCCCGTGCCGCAGCAGTTCTCACTCAGCCAGAACGGCCTGGTTAACGCAGCAGATAACTATCAGAACGCGGGCTGGCGCACCTTTTTTGTTGATAATCAGGTGAAGACGCTGATTAG  >  NC_000913/595535‑595802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑