Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 627,410 C→T I447I (ATC→ATT)  entE → 2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913627,4100CT95.3% 131.5 / ‑2.6 43I447I (ATC→ATT) entE2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (21/20);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGT  >  NC_000913/627277‑627542
                                                                                                                                     |                                                                                                                                    
gcgcCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAAc                                                                                                                                >  1:194402/1‑140 (MQ=255)
gcgcCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAAc                                                                                                                                >  1:56821/1‑140 (MQ=255)
gcgcCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCATGGCGGCCAGAGGAGCGCTGCGGAAGAGATTGAAAAc                                                                                                                                >  1:259944/1‑140 (MQ=255)
     tttGATGCCAACGGTGTTTACTTTTACGGGGATCTGAGCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACctgct                                                                                                                           <  1:329264/140‑1 (MQ=255)
           gCCAACGGTTTTTACTGTTCCGGCGATCTGACCTCTATTCATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTgcg                                                                                                                     <  2:279289/140‑1 (MQ=255)
              aaCGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGTTCGCTGCAGAAAAGATCGAAAACCTGCTGCTGCGCCa                                                                                                                  >  1:339578/1‑140 (MQ=255)
                     tttACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCcggcg                                                                                                           <  1:162242/140‑1 (MQ=255)
                               cGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcg                                                                                                 <  1:26729/140‑1 (MQ=255)
                                  cGATCTGATCTCTATTGATCCATAGTGTTCCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcgccg                                                                                              <  1:277257/140‑1 (MQ=255)
                                  cGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcgccg                                                                                              <  2:725612/140‑1 (MQ=255)
                                         atctCTATTGATCCAGATGGTTGCATCACCGAGCACGGGTGCGAGAAAAATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGt                                                                                       <  2:260261/140‑1 (MQ=255)
                                         atctCTATTGATCCAGATGGTTACATCACCGTGCATGGGGGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGt                                                                                       <  2:339578/140‑1 (MQ=255)
                                          tctcTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTg                                                                                      <  2:194402/140‑1 (MQ=255)
                                              tATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCa                                                                                  >  1:485598/1‑140 (MQ=255)
                                              tATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCa                                                                                  >  2:768639/1‑140 (MQ=255)
                                               aTTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGAGCCACCCGGCGCTGATCTACGCCGCACTGGTGAGCAt                                                                                 >  1:332730/1‑140 (MQ=255)
                                                 tGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGACAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCTCCCGGCGGTGATCTACGCCGCACTGGAGAGCATgg                                                                               <  2:40788/140‑1 (MQ=255)
                                                   aTCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTTCTGCTGCGCCACGCGGTGGTGATCTACGCCCCACTGGTGAGCATGGaa                                                                             >  2:337597/1‑140 (MQ=255)
                                                   aTCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGaa                                                                             >  2:111532/1‑140 (MQ=255)
                                                    cccggagGGTTACATCACCGTGCCGGGGCGCCAGACAGAAGAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCGCCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAg                                                                            <  2:259944/136‑1 (MQ=255)
                                                        gagGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGt                                                                                       >  1:435145/1‑125 (MQ=255)
                                                        gagGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGt                                                                                       <  2:435145/125‑1 (MQ=255)
                                                        gagGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGa                                                                        >  2:620773/1‑140 (MQ=255)
                                                        gagGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCCGCGCCACCAGGCCGTGATCTACGCCGCACTTGTGAGCATGGAAGATGa                                                                        >  2:368882/1‑140 (MQ=255)
                                                             ttACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCa                                                                                  >  1:141356/1‑125 (MQ=255)
                                                             ttACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCa                                                                                  <  2:141356/125‑1 (MQ=255)
                                                             ttACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGa                                                                   <  1:768639/140‑1 (MQ=255)
                                                                cATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATgg                                                                <  1:620773/140‑1 (MQ=255)
                                                                     ccGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGaa                                                           <  2:56821/140‑1 (MQ=255)
                                                                      cGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGGGCTGATGGGCGaaa                                                          >  1:131614/1‑140 (MQ=255)
                                                                       gTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGaaaa                                                         >  1:558439/1‑140 (MQ=255)
                                                                        tGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGGGATCTACGCCGCACTGGTGGGCATGGGAGATGAACTGATGGGCGaaaaa                                                        >  1:290797/1‑140 (MQ=255)
                                                                          cAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAg                                                      >  2:757475/1‑140 (MQ=255)
                                                                              ggCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTgc                                                  <  2:558439/140‑1 (MQ=255)
                                                                                cgcgAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTgcgc                                                >  1:671495/1‑140 (MQ=255)
                                                                                  cgAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCtt                                              >  1:15143/1‑140 (MQ=255)
                                                                                    agaAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTAt                                            <  2:671495/140‑1 (MQ=255)
                                                                                     gaAAGATCAGATTAACCGTGGCTGCTAGATGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATc                                           <  1:337597/140‑1 (MQ=255)
                                                                                         gATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCtggt                                       <  2:15143/140‑1 (MQ=255)
                                                                                         gATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCtggt                                       <  1:522904/140‑1 (MQ=255)
                                                                                                ttAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTaaaa                                >  2:456232/1‑140 (MQ=255)
                                                                                                         gcggcgAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGGGAAGAAAACTGCGCTTTTCTGGTGGTAAAAGAGCCGCTg                       >  2:245113/1‑140 (MQ=255)
                                                                                                         gcggcgAGAAGATCGCTGCCGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTg                       >  2:603547/1‑140 (MQ=255)
                                                                                                             cgAGAAGATCGCTGCCGCAGAGTTTGAAAACATGCGGCTCCGCCACCCCGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTgcgcg                   <  2:290797/140‑1 (MQ=255)
                                                                                                                         tGCCGAAGAGATTGACAACCTGCTGATGCCCCACCCGCCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGc       <  1:368882/140‑1 (MQ=255)
                                                                                                                           ccGAAGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGcgt     >  2:793768/1‑140 (MQ=255)
                                                                                                                              aaGAGATTGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGcgtcgt  <  1:793768/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGT  >  NC_000913/627277‑627542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑