Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	640,890	C→T	H380Y (CAC→TAC)	ahpF →	alkyl hydroperoxide reductase, AhpF component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	640,890	0	C	T	100.0%	92.9 / NA	29	H380Y (CAC→TAC)	ahpF	alkyl hydroperoxide reductase, AhpF component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/24);  total (5/24)

TCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCAC  >  NC_000913/640757‑640996                                                                                                                                      |                                                                                                            tCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTaa                                                                                                       <  1:822516/140‑1 (MQ=255)   aGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACg                                                                                                     <  2:637822/140‑1 (MQ=255)       tCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACgctgc                                                                                                 >  1:22246/1‑140 (MQ=255)          cACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTTAGCAGGGAACGCCGCTgg                                                                                              >  1:547915/1‑140 (MQ=255)          cACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGGTGGGGACGTAAAGCTGCTgg                                                                                              >  2:2167/1‑140 (MQ=255)          cACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGGTGAGTACGCAACgcctccgg                                                                                              >  2:188942/1‑134 (MQ=255)          cACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATCGACCTGGGGGGCATGGGTTAGCACGGAACGCTGCTgg                                                                                              >  1:362825/1‑140 (MQ=255)             cAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAAt                                                                                           >  1:831104/1‑140 (MQ=255)                   cGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTgcgc                                                                                     <  1:112762/140‑1 (MQ=255)                     tGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCa                                                                                   <  2:13847/140‑1 (MQ=255)                          tACTGCCCGCACTGCGAGGGCCCGCTGTTTAGAGGTAAAGGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAAt                                                                              <  2:362825/140‑1 (MQ=255)                                 cGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGGTTGGGACGGAACGCTGCTTGAAATTTCGCCCGAAATGTAAAGc                                                                       >  2:562367/1‑140 (MQ=255)                                   cACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTGGGTACCTAACGCTGCTGGGATTTTTGCCAAGaaag‑aaagacca                                                                    >  2:171810/1‑136 (MQ=255)                                   cACTGCGACGGCCCGATGTTTAAAGGTAAACGCGTAGAGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGa                                                                    <  1:413467/140‑1 (MQ=255)                                        cGACGGCCCGCTGTTTTAAGGTCAACGCGTCCCTGCTATCGGCGGCGGAAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAgg                                                               <  2:415451/140‑1 (MQ=255)                                        cGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAgg                                                               <  1:2167/140‑1 (MQ=255)                                            ggCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCt                                                           <  2:22246/140‑1 (MQ=255)                                            ggCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCt                                                           <  1:802666/140‑1 (MQ=255)                                              cccGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGGTTGTTGGGTAAAGAAAGCTGGTGGGAATTTCGCCCGAAAAG‑AAAACCGACCCGGGTCTGc                                                         >  2:53437/1‑140 (MQ=255)                                                           ggTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTgcg                                            <  2:193594/140‑1 (MQ=255)                                                           ggTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTgcg                                            <  2:89178/140‑1 (MQ=255)                                                           ggTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTCCAGGACAAACTgcg                                            <  2:831104/140‑1 (MQ=255)                                                           ggTAAACGCGTAGCGGTTAGCGGCGGCGGTAACTCCGGCGTGGAAGCGACAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTgcg                                            <  1:28673/140‑1 (MQ=255)                                                              aaaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAg                                         <  1:171810/140‑1 (MQ=255)                                                               aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGTGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGc                                        >  1:61156/1‑140 (MQ=255)                                                               aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGGATCGTTGGGGACGTAACGCTGCTGGAATTTTGGCCCGAAAAG‑AAAAACGACCAAGTTCTGGCGGACAAAAATCGCAGc                                        >  2:21212/1‑140 (MQ=255)                                                                          tttATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGt                             <  1:596866/139‑1 (MQ=255)                                                                          gTTATCGGCGGCGGTAAGTCCGTCGTGGAAGTGGCCATTTACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGt                             <  2:317484/140‑1 (MQ=255)                                                                          gTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGt                             <  1:53437/140‑1 (MQ=255)                                                                                       gTAACTCCGGCGTGGAAGCGGCAATTGACCTGGTGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTg                <  2:61156/140‑1 (MQ=255)                                                                                        tAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGa               <  1:188942/140‑1 (MQ=255)                                                                                             ccGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATgcg          <  1:714834/140‑1 (MQ=255)                                                                                                  gTGGAAGCGGCAATTTACCTGGAGGCTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAAc     <  1:220182/140‑1 (MQ=255)                                                                                                    ggAAGCGGCAATTGATCTGGCGCGTGTCGTTGAGTACTTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCa   <  1:300593/140‑1 (MQ=255)                                                                                                    ggAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACGAACTGCGCGGCCAGAAAAACGTCGAAATTAGTCTGAATGCGCAAACCa   >  2:258309/1‑140 (MQ=255)                                                                                                    ggAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCa   <  1:125966/140‑1 (MQ=255)                                                                                                     gAAGCGGCAATTGACCTGGCGGGTATCGTTGAGTACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGGTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCAc  >  1:63145/1‑140 (MQ=255)                                                                                                                                      |                                                                                                            TCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATG‑AAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCAC  >  NC_000913/640757‑640996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑