Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,415	T→C	intergenic (‑3/+115)	rihA ← / ← gltL	pyrimidine‑specific ribonucleoside hydrolase RihA/glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,415	0	T	C	100.0%	119.3 / NA	35	intergenic (‑3/+115)	rihA/gltL	pyrimidine‑specific ribonucleoside hydrolase RihA/glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

GTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCG  >  NC_000913/684279‑684548                                                                                                                                         |                                                                                                                                      gTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATtgctgc                                                                                                                                    >  1:105864/1‑140 (MQ=255)       tCTGGTGTTTGGTTTCTGGCGGGAGACGTACTTTCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCttg                                                                                                                              <  2:422411/140‑1 (MQ=255)       tCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCttg                                                                                                                              <  1:840708/140‑1 (MQ=255)           gtgtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTtgttgt                                                                                                                          >  2:828547/1‑140 (MQ=255)                     tCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATCGGCAGTGGCATTGCGGCTCCTAGTTGTGTGCTTCttt                                                                                                                >  1:413248/1‑140 (MQ=255)                          cggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGAc                                                                                                           <  1:805505/140‑1 (MQ=255)                           ggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACa                                                                                                          <  2:590733/140‑1 (MQ=255)                                       tGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCa                                                                                              <  2:105864/140‑1 (MQ=255)                                         cTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGTCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGt                                                                                                     <  2:531469/131‑1 (MQ=255)                                         cTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGt                                                                                                     >  1:531469/1‑131 (MQ=255)                                         cTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCAATTGCTGCTCCTGGTTCTGTGCTTCGTTGCGACAAGGGTAACGCCAgg                                                                                            >  2:228029/1‑140 (MQ=255)                                             gACATCAAGCTCTGGCGAGGCGAGGCCGACAACTATTCCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGt                                                                                        <  1:228029/140‑1 (MQ=255)                                                       tctGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACg                                                                              <  1:828547/140‑1 (MQ=255)                                                        ttGGTGAGTCGAGGGCGTGAGCGATTGCGATAGCGCCGTAATGCCCTGAGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGa                                                                             <  2:413248/139‑1 (MQ=255)                                                        ctGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGa                                                                             >  1:103418/1‑140 (MQ=255)                                                          ggTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAgg                                                                           >  1:152767/1‑140 (MQ=255)                                                               ggcgagggcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGa                                                                      <  1:216108/140‑1 (MQ=255)                                                                gcgagggcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAATAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTaa                                                                                      >  1:593397/1‑123 (MQ=255)                                                                gcgagggcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTaa                                                                                      <  2:593397/123‑1 (MQ=255)                                                                    gggcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGa                                                                 <  2:152767/140‑1 (MQ=255)                                                                      gcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATa                                                               <  2:623637/140‑1 (MQ=255)                                                                      gcgagAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATa                                                               <  2:528386/140‑1 (MQ=255)                                                                                ttGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTgaga                                                     >  2:788801/1‑140 (MQ=255)                                                                                        gCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGgcgcgc                                             >  1:626681/1‑140 (MQ=255)                                                                                                        ggTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATgcgcgc                             >  2:123345/1‑140 (MQ=255)                                                                                                        ggTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATgcgcgc                             >  2:681482/1‑140 (MQ=255)                                                                                                        ggTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATgcgcgc                             >  1:598806/1‑140 (MQ=255)                                                                                                        ggTCGCAATCTAACAGAATTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATgcgcgc                             >  2:448422/1‑140 (MQ=255)                                                                                                                        aaTTGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCa             >  2:156875/1‑140 (MQ=255)                                                                                                                          ttGGCAGTGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTTGGTATGGGCGCCAGAGTGATTAATGCAgg           >  1:422892/1‑140 (MQ=255)                                                                                                                                 tGCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTcg    <  1:156875/140‑1 (MQ=255)                                                                                                                                  gCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAAt                >  1:211943/1‑127 (MQ=255)                                                                                                                                  gCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAAt                <  2:211943/127‑1 (MQ=255)                                                                                                                                  gCCATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTcgc   >  1:801332/1‑140 (MQ=255)                                                                                                                                   ccATTGCTGTTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTcgcg  <  2:502693/140‑1 (MQ=255)                                                                                                                                   ccATTGCTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTcgcg  <  1:681482/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCG  >  NC_000913/684279‑684548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑