Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,873	A→G	V116A (GTA→GCA)	coaE ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,873	0	A	G	100.0%	161.6 / NA	45	V116A (GTA→GCA)	coaE	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/27);  total (18/27)

TTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTACCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCG  >  NC_000913/112739‑113009                                                                                                                                       |                                                                                                                                         ttGCCTGAGCAGCAAGGATTTGCTCTACAGGCTCGCGTGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGc                                                                                                                                     <  1:352754/140‑1 (MQ=255)        agcagcAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGTTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGca                                                                                                                              <  1:623557/140‑1 (MQ=255)        agcagcAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGca                                                                                                                              <  1:170461/140‑1 (MQ=255)        agcagcAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGca                                                                                                                              <  2:822992/140‑1 (MQ=255)        agcagcAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGca                                                                                                                              <  1:587006/140‑1 (MQ=255)          cagcaAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            <  2:137434/140‑1 (MQ=255)          cagcaAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            <  2:821538/140‑1 (MQ=255)            gcaAGGATTTGTTCGACATGCTTGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAAc                                                                                                                          <  1:386903/140‑1 (MQ=255)            gcaAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAAc                                                                                                                          <  2:623702/140‑1 (MQ=255)            gcaAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAAc                                                                                                                          <  2:774645/140‑1 (MQ=255)              aaGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTGTTATACAATGACTTGTCTGCCAGCAATGGCACAAccc                                                                                                                        >  1:411753/1‑140 (MQ=255)               aGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            <  2:677166/135‑1 (MQ=255)               aGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            >  1:584339/1‑135 (MQ=255)               aGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            <  2:584339/135‑1 (MQ=255)               aGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            >  1:677166/1‑135 (MQ=255)               aGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCca                                                                                                                       <  1:86888/140‑1 (MQ=255)                      gTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACa                                                                                                                <  1:520331/140‑1 (MQ=255)                           aCATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATCGCACAACCCACAGTACATAggg                                                                                                           >  1:374895/1‑140 (MQ=255)                            cATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            <  1:597882/122‑1 (MQ=255)                            cATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGcaca                                                                                                                            >  2:597882/1‑122 (MQ=255)                             aTGCTCGCGAGTTACGTCATCGAGCTGCAGGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAggggg                                                                                                         <  1:368603/140‑1 (MQ=255)                                      aGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCtt                                                                                                <  1:22785/140‑1 (MQ=255)                                      aGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCtt                                                                                                <  1:466847/140‑1 (MQ=255)                                        ttACATCACCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCCCAAGCACTCGATTCGCTTTTTAATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGCAAGAAGCTTga                                                                                              <  2:157653/140‑2 (MQ=255)                                             tcatcGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTgggt                                                                                         <  1:676834/140‑3 (MQ=255)                                             tcatcGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGAt                                                                                         <  1:647927/140‑1 (MQ=255)                                                tcGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGc                                                                                              <  1:80697/132‑1 (MQ=255)                                                tcGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGc                                                                                              >  2:80697/1‑132 (MQ=255)                                                tcGCGCTGCATGGTGCCCTTAACTTGCGTTCCTTGCCTGACATCCACCCCAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTg                                                                                      <  2:411753/140‑1 (MQ=255)                                                     cTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCGACACTACATAGGGGGAGGTAGTTTGCTGGATCTGGTGtt                                                                                 >  2:286490/1‑140 (MQ=255)                                                                           gTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCgg                                                           <  1:771470/140‑1 (MQ=255)                                                                            tttCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGa                                                          <  1:37021/140‑1 (MQ=255)                                                                              tCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATg                                                        >  1:444197/1‑140 (MQ=255)                                                                                 gggCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATgcag                                                     >  1:35387/1‑140 (MQ=255)                                                                                    cTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGcagcag                                                  <  2:788999/140‑1 (MQ=255)                                                                                                         cGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTtctc                             >  2:629654/1‑140 (MQ=255)                                                                                                         cGATTCGCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTtctc                             >  2:164059/1‑140 (MQ=255)                                                                                                               gCTTTTTTATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTCGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCgg                       >  2:435819/1‑140 (MQ=255)                                                                                                                 ttttttATACAGTGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTATGCCAGTTTTTCTCTTCCGGGt                     <  2:145702/140‑1 (MQ=255)                                                                                                                             tGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATcc         >  2:452883/1‑140 (MQ=255)                                                                                                                             tGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATcc         >  1:640069/1‑140 (MQ=255)                                                                                                                             tGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATcc         >  2:165641/1‑140 (MQ=255)                                                                                                                             tGAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATcc         >  2:821089/1‑140 (MQ=255)                                                                                                                              gAGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCg        >  1:713804/1‑140 (MQ=255)                                                                                                                               aGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAGGCCGGTTTTTCTCTTCCGAGTTGGCGGAGATCCGc       >  2:418324/1‑140 (MQ=255)                                                                                                                               aGTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTATTCCGGGTTGGCGAAGATCCGc       >  1:387238/1‑140 (MQ=255)                                                                                                                                gTTTTCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCt      <  1:629654/140‑1 (MQ=255)                                                                                                                                    tCTGCCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAACCAGTTTTTCTCTTCCCGGTTCGCGAAGATCCGCTcgcg  >  2:337634/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGTTTTCTACCAGCAATGGCACAACCCACAGTACATAGGGGGAAGTAGCTTGCTGGATCTGGTGTTGCGTCTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCG  >  NC_000913/112739‑113009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑