Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	710,005	A→G	V72V (GTA→GTG)	chiQ →	lipoprotein ChiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	710,005	0	A	G	100.0%	173.7 / NA	50	V72V (GTA→GTG)	chiQ	lipoprotein ChiQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

TCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTC  >  NC_000913/709870‑710141                                                                                                                                        |                                                                                                                                         tcgtcTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCTGCAGTTCGCCGACTAGGAAAGTGTGcgcg                                                                                                                                      >  1:401668/1‑140 (MQ=255) tcgtcTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGcgcg                                                                                                                                      >  1:550530/1‑140 (MQ=255)              gCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGAt                                                                                                                              >  1:507798/1‑135 (MQ=255)              gCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGAt                                                                                                                              <  2:507798/135‑1 (MQ=255)              gCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATca                                                                                                                         >  1:573469/1‑140 (MQ=255)                        ccTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCGGGAAAGTGTGCGCGGACTGGAATATCAGCAGTGTCTg                                                                                                               >  1:225571/1‑140 (MQ=255)                        ccTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTg                                                                                                               >  1:534190/1‑140 (MQ=255)                         cTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATcagcag                                                                                                                     <  1:155073/133‑1 (MQ=255)                         cTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATcagcag                                                                                                                     >  2:155073/1‑133 (MQ=255)                            tATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTg                                                                                                           >  2:446526/1‑140 (MQ=255)                              tCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCa                                                                                                         >  1:20935/1‑140 (MQ=255)                                 acacCGCACAAGGTTCGCCAGACAAAATTGATGCCCGCCCGAGCGTGTCAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACg                                                                                                      <  2:225571/140‑1 (MQ=255)                                 acacCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACg                                                                                                      <  2:567006/140‑1 (MQ=255)                                   acCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCa                                                                                                    >  1:694459/1‑140 (MQ=255)                                      gCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAc                                                                                                 >  2:839507/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  2:185038/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  2:574674/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  2:483351/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  1:567382/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  1:852924/1‑140 (MQ=255)                                       cacaAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAAcc                                                                                                >  1:779145/1‑140 (MQ=255)                                                cGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGAt                                                                                       <  1:618401/140‑1 (MQ=255)                                                cGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGAt                                                                                       <  1:483351/140‑1 (MQ=255)                                                 gCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATc                                                                                      >  2:126218/1‑140 (MQ=255)                                                         aaaTTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTg                                                                              <  2:852924/140‑1 (MQ=255)                                                             tGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAg                                                                          <  1:839507/140‑1 (MQ=255)                                                               aaGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGcc                                                                        <  2:20935/140‑1 (MQ=255)                                                                aGCCTGCCAGAGCGTGTTAAACGTGCTGAAGATAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCg                                                                       >  1:776354/1‑140 (MQ=255)                                                                 gCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGa                                                                      <  1:185038/140‑1 (MQ=255)                                                                   cTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGAtt                                                                    <  1:126218/140‑1 (MQ=255)                                                                       cAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGa                                                                <  2:677012/140‑1 (MQ=255)                                                                             gtgtTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGt                                                          <  2:550530/140‑1 (MQ=255)                                                                                ttAAACGTGCTGAAGAAGGAGACACAACATCTGCATTTTCCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTg                                                       <  2:282687/140‑1 (MQ=255)                                                                                ttAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAAGCGGTGAAAGCCGATTGTGATAAAGTCTg                                                       >  1:282687/1‑140 (MQ=255)                                                                                  aaaCGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGc                                                     <  1:705640/140‑1 (MQ=255)                                                                                  aaaCGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGc                                                     <  1:103359/140‑1 (MQ=255)                                                                                     cGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAgg                                                  <  2:573469/140‑1 (MQ=255)                                                                                        gCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGaaa                                               >  1:541003/1‑140 (MQ=255)                                                                                         cTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAt                                              >  2:222937/1‑140 (MQ=255)                                                                                          tGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATa                                             <  2:779145/140‑1 (MQ=255)                                                                                           gaagaaAGAGAAACAACGTCAGGAGTTTGCCGACCAGGATAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATAc                                            <  2:401668/140‑1 (MQ=255)                                                                                                  gagaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGtaa                                     <  2:694459/140‑1 (MQ=255)                                                                                                  gagaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGtaa                                     <  2:92292/140‑1 (MQ=255)                                                                                                    gaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGtaata                                   <  2:115155/140‑1 (MQ=255)                                                                                                    gaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGtaata                                   <  2:764016/140‑1 (MQ=255)                                                                                                      aacaacATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAAc                                 <  2:567382/140‑1 (MQ=255)                                                                                                      aacaacATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAAc                                 >  1:746297/1‑140 (MQ=255)                                                                                                      aacaacATCAGCAGTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAAc                                 >  1:138321/1‑140 (MQ=255)                                                                                                                 cagTTTGCCGACCAGGAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTgag                      <  1:574674/140‑1 (MQ=255)                                                                                                                               ggAAAGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCg        <  2:746297/140‑1 (MQ=255)                                                                                                                                 aaaGTGTGCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCt      <  1:446526/140‑1 (MQ=255)                                                                                                                                     tgtgCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGTAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAActct          <  2:734312/132‑1 (MQ=255)                                                                                                                                     tgtgCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGTAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAActct          >  1:734312/1‑132 (MQ=255)                                                                                                                                     tgtgCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTc  >  2:69880/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTC  >  NC_000913/709870‑710141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑