Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,004	A→G	T483A (ACT→GCT)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,004	0	A	G	98.0%	175.3 / NA	50	T483A (ACT→GCT)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/22);  minor base C (0/1);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATC  >  NC_000913/714878‑715139                                                                                                                               |                                                                                                                                        ggTGCGCCGAGCTACTACCGTTAGCCGGCTGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCggg                                                                                                                            <  1:272654/140‑1 (MQ=255) ggTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCggg                                                                                                                            <  1:820357/140‑1 (MQ=255)      gcCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACg                                                                                                                       <  2:624662/140‑1 (MQ=255)        cGAGCTACAGCCGTTTGAAGGCTGCTGCGACTTCCCCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGt                                                                                                                     <  2:323628/140‑1 (MQ=255)                     tttGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGTCTGGCTGATGCCCCAGGCAACTGTGCTTCTATTGGCg                                                                                                        >  1:345592/1‑140 (MQ=255)                     tttGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCg                                                                                                        >  1:744430/1‑140 (MQ=255)                     tttGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCg                                                                                                        >  2:596740/1‑140 (MQ=255)                      ttGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCGTGGCTGCGGCTACGGGCAACGGTGCTTCTATTGGCgg                                                                                                       >  1:224440/1‑140 (MQ=255)                          aggcagCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTg                                                                                                   <  1:522864/140‑1 (MQ=255)                            gcagCTGCGACTTCCGCCCAAACAGCGGCGCTGTCTAAGTTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGaa                                                                                                 <  2:264082/140‑1 (MQ=255)                                        tCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGTTGCTCCGGACAACGGTTCTTCTATTGGCGGATTGAAAGGTAtgactga                                                                                     >  2:408602/1‑140 (MQ=255)                                                        cgcTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTcgcc                                                                     >  2:507585/1‑140 (MQ=255)                                                                    tGTCTCCGGAAATGGTGCGCGCGAGCTCCCGCGCACGTGACCCGATCAGCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCa                                                         <  2:345592/140‑1 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGc                                                      >  2:574392/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGc                                                      >  2:39762/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGc                                                      >  2:542534/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGc                                                      >  1:595189/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGc                                                      >  2:30430/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGGCTGAAAGGGATGACCGTAAACGGCTGGTTTGACGCGCGTTCGTCAGGc                                                      >  2:423553/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGACTGAAAGTAGTGACTGGCAACGGCTCGTTTACCGCGCGTCCGTCAGGc                                                      >  2:281519/1‑140 (MQ=255)                                                                       ctcCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGGGCTTCTATTGGCGGGCTGAAAGCGATGAGTCACAACGGGTGGGTCCCCGGCCGGCCGTCAggg                                                      >  2:586330/1‑139 (MQ=255)                                                                         ccGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCAc                                                    >  1:749977/1‑140 (MQ=255)                                                                         ccGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCAc                                                    >  1:93182/1‑140 (MQ=255)                                                                             aaaTGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAgg                                                       <  1:553367/133‑1 (MQ=255)                                                                             aaaTGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAgg                                                       >  2:553367/1‑133 (MQ=255)                                                                               aTGGTGAGCGCCAGCTCCCTGGTAGTTGAGCCGATCATCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGa                                              <  1:281519/140‑1 (MQ=255)                                                                               aTGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGa                                              <  2:698407/140‑1 (MQ=255)                                                                               aTGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGa                                              <  2:744430/140‑1 (MQ=255)                                                                               aTGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGa                                              <  1:507585/140‑1 (MQ=255)                                                                               aTGGTGAGCGCCAGCACCCTGGAAGGTGACCTGATTACCCCGCGCCTGCTTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGa                                              <  2:224440/140‑1 (MQ=255)                                                                                  gTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGc                                           >  1:28029/1‑140 (MQ=255)                                                                                    gAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCat                                         <  2:431323/140‑1 (MQ=255)                                                                                    gAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCat                                         <  2:93182/140‑1 (MQ=255)                                                                                    gAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCat                                         >  1:431323/1‑140 (MQ=255)                                                                                    gAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCat                                         <  1:669217/140‑1 (MQ=255)                                                                                    gAGCGCCAGCACCCTAGCAGGTCACTCGAACACCGCGGGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCat                                         <  1:408602/140‑1 (MQ=255)                                                                                                        gTGACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGGTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGc                     >  2:161804/1‑140 (MQ=255)                                                                                                          gACCCGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCtt                   >  1:755292/1‑140 (MQ=255)                                                                                                             ccGATCACCGCGCGCTTGGCTGCTGATCCTGGCAACGTTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTAAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCt                <  1:265952/140‑1 (MQ=255)                                                                                                             ccGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGGGAAAGCTTCCt                <  1:596740/140‑1 (MQ=255)                                                                                                             ccGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCt                <  1:542534/140‑1 (MQ=255)                                                                                                              cGATCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGGGATGACTGACAACCGGTGGGTCGCCGCCGGTGCGCTAGGCACCGAAAACGCATATAAACTCTTCTACGAAAGATTcccc               >  2:153554/1‑138 (MQ=255)                                                                                                                aTCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGc                           <  2:436417/126‑1 (MQ=255)                                                                                                                aTCACCGCGCGCCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGc                           >  1:436417/1‑126 (MQ=255)                                                                                                                    ccGCGCGCCTGGCTGCTGCTCCTGGAAACTGTGCTTCCATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTgaa         <  1:423553/140‑1 (MQ=255)                                                                                                                      gcgcgcCTGGCTGCTGTTCCGGGCAACGGTGGTTCTATTTGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTgaaga       <  2:397157/140‑1 (MQ=255)                                                                                                                      gcgcgcCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGGTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAAATCCACAGCGAAAACTTCCTCGGTgaaga       >  1:397157/1‑140 (MQ=255)                                                                                                                       cgcgcCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGGAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGGgaagaa      >  2:206977/1‑140 (MQ=255)                                                                                                                          gcCTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACAt   <  1:586330/140‑1 (MQ=255)                                                                                                                           ccTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGGACGGGAGAAGCATATAAGATTTACTGCGACAGCTTCCTCGGTGAAGAACATc  >  2:350146/1‑140 (MQ=255)                                                                                                                           ccTGGCTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATc  >  1:18668/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                        GGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATC  >  NC_000913/714878‑715139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑