Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	771,858	C→T	A134V (GCG→GTG)	cydA →	cytochrome bd‑I ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	771,858	0	C	T	100.0%	191.5 / NA	57	A134V (GCG→GTG)	cydA	cytochrome bd‑I ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

AGGGGATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAA  >  NC_000913/771721‑771987                                                                                                                                          |                                                                                                                                  aGGGGATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCGGCAGATATGTGTCGCCTGGCTGGTGgtgc                                                                                                                                 >  1:223257/1‑140 (MQ=38)  ggggATATCTTCGGTGCTCCGCCGGCAATCGAAGGGCTGCTGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                <  2:321609/140‑1 (MQ=38)  ggggATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                <  1:586011/140‑1 (MQ=255)  ggggATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                >  1:66543/1‑140 (MQ=255)  ggggATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                <  1:468440/140‑1 (MQ=255)  ggggATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                <  2:156619/140‑1 (MQ=255)   gggATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAGAGTTCAGCATAAGTGTGTCACTTGGCTGGTGGTGCTc                                                                                                                               >  1:321609/1‑140 (MQ=255)      atatCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGt                                                                                                                            >  1:694945/1‑140 (MQ=255)      atatCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGAGGTGCTCGGt                                                                                                                            >  1:91560/1‑140 (MQ=255)        atCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCAGGGTAAAGTTCAGCATAAGTGTGTCGCCTGGATGGTGGTACTCGGTTc                                                                                                                          >  2:413603/1‑140 (MQ=38)          cTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCaa                                                                                                                        >  2:64968/1‑140 (MQ=255)             cGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                <  2:689808/129‑1 (MQ=255)             cGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCt                                                                                                                                >  1:689808/1‑129 (MQ=255)                tGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGt                                                                                                                  <  2:95128/140‑1 (MQ=255)                  cgccgcTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTACAGCATATGTGTTTCAACTGGCTGGTGGTGGTCGGTTCAAACCTGTcc                                                                                                                >  2:222330/1‑140 (MQ=38)                  cgccgcTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGGAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTcc                                                                                                                >  2:616141/1‑140 (MQ=255)                   gccgcTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCg                                                                                                               >  2:57273/1‑140 (MQ=255)                      gcTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCAc                                                                                                            <  2:137258/140‑1 (MQ=255)                       cTGGCAATCGAAGGTGTGATGGTCTTCTTTCTCGAATGCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACt                                                                                                           <  1:413603/140‑1 (MQ=255)                       cTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACt                                                                                                           >  1:557260/1‑140 (MQ=255)                                       tGATGGCCTTCTTCCGCGAATCGACATTTATAGGTCTATTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCg                                                                                           <  1:276048/140‑1 (MQ=255)                                         aTGGCCTTCGTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCGGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGCGGTGCTCGGGTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGaa                                                                                         >  2:768323/1‑140 (MQ=255)                                              cttcttCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCt                                                                                    >  1:177947/1‑140 (MQ=255)                                                     cTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCa                                                                             <  2:557260/140‑1 (MQ=255)                                                     cTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCa                                                                             <  2:19955/140‑1 (MQ=255)                                                     cTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGAGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCa                                                                             <  2:91560/140‑1 (MQ=255)                                                     cTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTAGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCa                                                                             <  2:539049/140‑1 (MQ=255)                                                       cGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCaaa                                                                           >  1:821261/1‑140 (MQ=255)                                                            ccACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTGTGGATGAGAACGGTTGGATGCAAAACCCa                                                                      >  1:262757/1‑140 (MQ=255)                                                                    gTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTc                                                              >  2:142886/1‑140 (MQ=255)                                                                        gTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGAt                                                          >  2:551281/1‑140 (MQ=255)                                                                          cTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGTTGGATGCAAAACCCAATCGCGTCCGAttt                                                        >  2:632986/1‑140 (MQ=255)                                                                             ttcttcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCaa                                                     >  1:677330/1‑140 (MQ=255)                                                                                  cttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTg                                                >  1:449435/1‑140 (MQ=255)                                                                                  cttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTg                                                >  1:670586/1‑140 (MQ=255)                                                                                     cGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGaaa                                             >  2:31965/1‑140 (MQ=255)                                                                                        ttGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTa                                          <  2:694945/140‑1 (MQ=255)                                                                                        ttGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTa                                          <  2:66543/140‑1 (MQ=255)                                                                                         tGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTAt                                         <  1:57273/140‑1 (MQ=255)                                                                                         tGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTAt                                         <  1:551281/140‑1 (MQ=255)                                                                                          gggATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGGTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATg                                        <  1:768323/140‑1 (MQ=255)                                                                                              tCGTCTGGGTAAAGTTCTGCATATGTGTGTCACCTTGCTCGTGGTGCTTGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTa                                    <  2:223257/140‑1 (MQ=255)                                                                                              tCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTa                                    <  2:177947/140‑1 (MQ=255)                                                                                              tCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTa                                    <  2:677330/140‑1 (MQ=255)                                                                                                  cTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGga                                >  2:733304/1‑140 (MQ=255)                                                                                                  cTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGga                                <  1:31965/140‑1 (MQ=255)                                                                                                      gTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATg                            <  1:142886/140‑1 (MQ=255)                                                                                                        aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGt                          <  1:427006/140‑1 (MQ=255)                                                                                                            ttCAGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGc                      <  2:183823/140‑1 (MQ=255)                                                                                                               aGCATATGTGTGTCACCTGGCTTGTGGTGCTCGGTTCAACCCTGTCCGAACTGTGGATTCTGGTTGCGAACG‑‑‑‑‑‑‑GCAAACCCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGc            <  2:225456/140‑1 (MQ=255)                                                                                                               aGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTtc                   >  2:38332/1‑140 (MQ=255)                                                                                                               aGCATATGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTtc                   >  2:733866/1‑140 (MQ=255)                                                                                                                  atatGTGTGTCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTcc                <  1:616141/140‑1 (MQ=255)                                                                                                                      gtgtgtCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGc            >  1:81252/1‑140 (MQ=255)                                                                                                                       tgtgtCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCt           <  1:38332/140‑1 (MQ=255)                                                                                                                         tgtCACCTGGCTGGTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACG‑‑‑‑‑‑‑GCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGAGTATGGAGATGTTGGGCTTCTACGAGCTGGTGCTTaa  >  1:225456/1‑140 (MQ=255)                                                                                                                               ctggctggTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATTCGTATGGGGATCGTGAGCTTCGCCGAGCTGGTGCTTa   >  1:371998/1‑140 (MQ=255)                                                                                                                                  gctggTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTgg         <  2:708195/131‑1 (MQ=255)                                                                                                                                  gctggTGGTGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTgg         >  1:708195/1‑131 (MQ=255)                                                                                                                                          |                                                                                                                                  AGGGGATATCTTCGGTGCGCCGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAA  >  NC_000913/771721‑771987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑