Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,181	A→G	Y264Y (TAT→TAC)	ybhG ←	HlyD_D23 family protein YbhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,181	0	A	G	100.0%	83.4 / NA	26	Y264Y (TAT→TAC)	ybhG	HlyD_D23 family protein YbhG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/21);  total (5/21)

GGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGCCTGGCTCCACCG  >  NC_000913/829046‑829317                                                                                                                                        |                                                                                                                                         ggTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTaaa                                                                                                                                      >  2:144336/1‑140 (MQ=255)            tACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATATGGCATGTACGGGCGACGATCGGGGTAAAGCAGCACTTTg                                                                                                                           >  2:384345/1‑140 (MQ=255)            tACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTg                                                                                                                           >  2:715596/1‑140 (MQ=255)                cAGGCGATAGACGAGGTCGGTAGGCCGATTCGGCGTTGCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGcc                                                                                                                       <  1:384345/140‑1 (MQ=255)                cAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGcc                                                                                                                       <  1:765486/140‑1 (MQ=255)                 aGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCCGTGTAAAGCAGCACTTTGCGccc                                                                                                                      >  1:671772/1‑140 (MQ=255)                 aGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTAATACGGCTGGTCTGGGTGACCATCGATGTACAGCAGCACTTTGCGccc                                                                                                                      >  2:343448/1‑140 (MQ=255)                 aGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTAGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGccc                                                                                                                      >  1:779859/1‑140 (MQ=255)                    cGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCgg                                                                                                                   >  2:773321/1‑140 (MQ=255)                          aCGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGc                                                                                                             <  1:773321/140‑1 (MQ=255)                           cGAGGTCGGTACGCATATCCGGTGTTTCGGCGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGcc                                                                                                            <  1:295483/140‑1 (MQ=255)                             aGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACGATCGGAGGAAAGCAGCCCTTTCGGCCCCGGCTGGGCCTg                                                                                                          >  2:393027/1‑140 (MQ=255)                               gTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGGGTAAAGCAGCACTTTGCGCCCCCGCTGGGCCCGGt                                                                                                        >  2:651266/1‑140 (MQ=255)                                    tACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGa                                                                                                   >  2:617725/1‑140 (MQ=255)                                         aGATCCGGCGTTTCGACGGTTTTCGGGGTTAATTCAGGAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACg                                                                                              <  1:393027/140‑1 (MQ=255)                                         aGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACg                                                                                              <  1:617725/140‑1 (MQ=255)                                         aGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACg                                                                                              <  1:837965/140‑1 (MQ=255)                                            tCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTtc                                                                                           <  1:651266/140‑1 (MQ=255)                                                           gTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCATTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAgcgcg                                                                            <  2:516451/140‑1 (MQ=255)                                                           gTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAgcgcg                                                                            <  2:438734/140‑1 (MQ=255)                                                              ttCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCACGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCAc                                                                         <  1:656230/140‑1 (MQ=255)                                                               tCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGcccc                                                                        <  2:834748/140‑1 (MQ=255)                                                                      aaaTTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCg                                                                 >  1:425878/1‑140 (MQ=255)                                                                        aTTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGa                                                               <  2:425878/140‑1 (MQ=255)                                                                             gcagTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGt                                                          <  1:575521/140‑1 (MQ=255)                                                                                  cGGCGAAACGAAACCAATCTGCACGTGATACAGTTTGTCAGGGCGACCCTCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTg                                                     <  2:404291/140‑1 (MQ=255)                                                                                    gCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGaa                                                   <  1:715596/140‑1 (MQ=255)                                                                                    gCGAAACGAAACCAATCTGCCCATGTTACGGCTTGTCTGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGaa                                                   <  1:343448/140‑1 (MQ=255)                                                                                             aacCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGGGTAAAGCAGCACTTTTCGCCCCCGCTGGGCCTGGGCAAGATTACGTTCCTCAACATAAACGTGACCCCACACCGCACGCTTTTGTGAAACGGTAAac                                          >  2:371724/1‑140 (MQ=255)                                                                                                         cGTGATACGGCTTGTTCGGGCGGCCATCGGTGTAAAGTAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCt                              <  1:371724/140‑1 (MQ=255)                                                                                                         cGTGATACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCt                              <  2:435423/140‑1 (MQ=255)                                                                                                              tACGGCTTGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCAtt                         <  2:779859/140‑1 (MQ=255)                                                                                                                    ttGTCCGGGCGACCATCGGTGTAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGAc                   <  2:671772/140‑1 (MQ=255)                                                                                                                                     ggTGTAAAGCAGCACCTTGCGCCCCCGCTCGGCCTGGCCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGCCTGGCTCCACcg  <  1:261937/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGCCTGGCTCCACCG  >  NC_000913/829046‑829317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑