Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	114,907	G→A	T273I (ACA→ATA)	hofC ←	inner membrane protein HofC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	114,907	0	G	A	100.0%	147.1 / NA	44	T273I (ACA→ATA)	hofC	inner membrane protein HofC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

CAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTGTCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTATTGGCTATCGC  >  NC_000913/114784‑115043                                                                                                                            |                                                                                                                                         cagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGt                                                                                                                          <  2:136225/140‑1 (MQ=255)    aTGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCATCGCATTGTTTCTCTGACGCTCTCTACGCGCTGTAAAATAGTAATGACTGCACTTAGTATGAGCGCCAGAATCGTaaa                                                                                                                       >  2:286984/1‑140 (MQ=255)              cGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGt                                                                                                              >  1:513364/1‑139 (MQ=255)              cGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGt                                                                                                              <  2:513364/139‑1 (MQ=255)                      atatCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTg                                                                                                     >  2:477538/1‑140 (MQ=255)                       tatCGTGCTGGATTTGAGTCAGAAGTTGCACCCGGTACGCGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGt                                                                                                    <  2:381344/140‑1 (MQ=255)                          cGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTccc                                                                                                 >  1:773482/1‑140 (MQ=255)                          cGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTccc                                                                                                 <  2:471387/140‑1 (MQ=255)                            tGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGt                                                                                                              <  2:598372/125‑1 (MQ=255)                            tGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGt                                                                                                              >  1:598372/1‑125 (MQ=255)                            tGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCg                                                                                               >  2:433421/1‑140 (MQ=255)                                 gATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTccc                                                                                                 <  1:586488/133‑1 (MQ=255)                                 gATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTccc                                                                                                 >  2:586488/1‑133 (MQ=255)                                   tttGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGt                                                                                        <  2:773482/140‑1 (MQ=255)                                       tgtCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAAc                                                                                    <  2:445054/140‑1 (MQ=255)                                                   cACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTACAGATCTGCGTGATTTTATGTCCCCGGATCAATGAACCCATAATCGGGa                                                                        >  2:265868/1‑140 (MQ=255)                                                        aGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGt                                                                   >  1:650510/1‑140 (MQ=255)                                                        aGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGt                                                                   <  2:775206/140‑1 (MQ=255)                                                                   cgcATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAAttt                                                        >  1:786844/1‑140 (MQ=255)                                                                         gTTTCTCTGACGCTCTCTACGCCCTGTAAAATAGTCATGCCTGCACTTAGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCg                                                  <  2:220602/140‑1 (MQ=255)                                                                         gTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCg                                                  <  2:23296/140‑1 (MQ=255)                                                                         gTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCg                                                  <  1:477538/140‑1 (MQ=255)                                                                             ctctGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACt                                              >  2:215723/1‑140 (MQ=255)                                                                             ctctGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACt                                              <  2:786844/140‑1 (MQ=255)                                                                                tGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACtata                                           <  1:215723/140‑1 (MQ=255)                                                                                  aCGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAGTCGGGATGCGTCACGGCAATTGCTGCCGCACTATAAg                                         >  1:220602/1‑140 (MQ=255)                                                                                    gCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCg                                                  >  2:20951/1‑129 (MQ=255)                                                                                    gCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCg                                                  <  1:20951/129‑1 (MQ=255)                                                                                    gCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGcc                                       >  2:702466/1‑140 (MQ=255)                                                                                            cGCCCTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAggtcggt                               >  1:775574/1‑140 (MQ=255)                                                                                              cccTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGgtcggtcg                             >  2:819049/1‑140 (MQ=255)                                                                                                cTGTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATTAGCCAGGTCGGTCGgc                           >  2:379458/1‑140 (MQ=255)                                                                                                  gTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGGGTGAGTTTTTTTCCCCGCATCAGCGAACCCATAATCGTGATGCGTAACAGCAATTTCTTCCGCACTATAAGCCAAATTGGTCGgcgc                         >  2:534686/1‑140 (MQ=255)                                                                                                  gTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGgtgc                         >  1:99888/1‑140 (MQ=255)                                                                                                  gTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGgcgc                         >  1:516806/1‑140 (MQ=255)                                                                                                  gTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGgcgc                         >  2:695450/1‑140 (MQ=255)                                                                                                  gTAAAAAAGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGgcgc                         <  1:819049/140‑1 (MQ=255)                                                                                                       aaaGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAg                    <  2:775574/140‑1 (MQ=255)                                                                                                        aaGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGc                   <  2:650510/140‑1 (MQ=255)                                                                                                        aaGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGc                   <  1:702466/140‑1 (MQ=255)                                                                                                         aGTAATGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCa                  <  1:433421/140‑1 (MQ=255)                                                                                                             aTGCCTGCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATGAGCGAGATCGGTCAGCGCATCAGCAACtt              >  1:271143/1‑140 (MQ=255)                                                                                                                   gCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTAt            >  2:533354/1‑136 (MQ=255)                                                                                                                   gCACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTAt            <  1:533354/136‑1 (MQ=255)                                                                                                                    cACTTTGTATCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTATTGGCt       >  1:716622/1‑140 (MQ=255)                                                                                                                         tgtaTCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTATTGGCTATCGc  >  2:62115/1‑140 (MQ=255)                                                                                                                         tgtaTCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGCCGGTCGGCGCATCAGCAACTTATTGGCTATCGc  >  1:435152/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                         CAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTGTCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTATTGGCTATCGC  >  NC_000913/114784‑115043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑