Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,465	T→A	I48F (ATC→TTC)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,465	0	T	A	100.0%	224.0 / NA	67	I48F (ATC→TTC)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/36);  total (31/36)

GACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGT  >  NC_000913/862331‑862600                                                                                                                                       |                                                                                                                                         gaCAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCt                                                                                                                                     <  1:168042/140‑1 (MQ=255)    aGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGAGCCAGTGACAGGGCGCGACGTACCGGGAACGGCTTAt                                                                                                                                  >  1:270081/1‑140 (MQ=255)      tataTTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCg                                                                                                                                >  1:18116/1‑140 (MQ=255)      tataTTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCg                                                                                                                                >  1:188356/1‑140 (MQ=255)      tataTTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCg                                                                                                                                <  1:71330/140‑1 (MQ=255)        tatTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCgag                                                                                                                              <  1:602831/140‑1 (MQ=255)        tatTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCgag                                                                                                                              <  2:40138/140‑1 (MQ=255)           tCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCATGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGTCTTATCGAGAtg                                                                                                                           >  1:591504/1‑140 (MQ=255)           tCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGAtg                                                                                                                           >  2:661595/1‑140 (MQ=255)           tCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGAtg                                                                                                                           >  1:491322/1‑140 (MQ=255)           tCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCACCACGCGACGTACCGGATACGGCTTATCGAGAtg                                                                                                                           >  2:396929/1‑140 (MQ=255)             cGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCgag                                                                                                                              <  2:618803/135‑1 (MQ=255)             cGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCgag                                                                                                                              >  1:618803/1‑135 (MQ=255)              gggAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACg                                                                                                                                        >  2:852758/1‑124 (MQ=255)              gggAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGAtgttg                                                                                                                        >  1:732228/1‑140 (MQ=255)              gggAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGAtgttg                                                                                                                        <  2:732228/140‑1 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  1:82278/1‑140 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  2:736442/1‑140 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  2:124797/1‑140 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  2:611840/1‑140 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  1:479549/1‑140 (MQ=255)                gAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATgttgtt                                                                                                                      >  2:680561/1‑140 (MQ=255)                  aGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGa                                                                                                                    >  2:728907/1‑140 (MQ=255)                  aGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTAGCGAGATGTTGTTGa                                                                                                                    <  2:820133/140‑1 (MQ=255)                    aTCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATa                                                                                                                  <  2:18116/140‑1 (MQ=255)                    aTCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATa                                                                                                                  <  1:661595/140‑1 (MQ=255)                    aTCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATa                                                                                                                  <  1:728907/140‑1 (MQ=255)                    aTCGGCGCGGCGCGAACTTCGCCTGTCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATa                                                                                                                  <  1:421973/140‑1 (MQ=255)                      cggcgcggcgCGAACTTCGCTTTCCTGGTTGACAACGATCAACTCATCTTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACa                                                                                                                <  2:252597/140‑1 (MQ=255)                      cggcgcggcgCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGCTGGTGCGATTCGCCAGGTGATGCGCCAGTCCCAGCGCGAGACATACAAGGAACGGTTTATCAAGATGTTTTTGATACa                                                                                                                >  2:421973/1‑140 (MQ=255)                       ggcgcggcgcGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACAt                                                                                                               <  2:82278/140‑1 (MQ=255)                         cgcggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACAtct                                                                                                             <  1:455490/140‑1 (MQ=255)                         cgcggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACAtct                                                                                                             <  2:479549/140‑1 (MQ=255)                         cgcggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACAtct                                                                                                             <  2:491322/140‑1 (MQ=255)                         cgcggcgcgAACTTCGCTTGCCAGGATGCCAATGATCTACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACAtct                                                                                                             <  1:400152/140‑1 (MQ=255)                            ggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCgg                                                                                                          <  1:736442/140‑1 (MQ=255)                              cgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGta                                                                                                        >  2:274688/1‑140 (MQ=255)                              cgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGta                                                                                                        >  1:835831/1‑140 (MQ=255)                                       cGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTGATCGAGATGTTTTTGATACATCTCGGTATAGtgctgc                                                                                               >  1:234374/1‑140 (MQ=255)                                         cTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTgcgc                                                                                             <  1:611840/140‑1 (MQ=255)                                            gCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTgcgcgcg                                                                                          <  1:680561/140‑1 (MQ=255)                                              cTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCt                                                                                        <  2:188356/140‑1 (MQ=255)                                              cTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCt                                                                                        <  2:39515/140‑1 (MQ=255)                                                           aTCAACTCATCGTGTTTCATCCTGATGGTGCGATTCGCCGGGTGATGCACCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTgg                                                                           <  2:234374/140‑1 (MQ=255)                                                            tCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGc                                                                          >  1:488330/1‑140 (MQ=255)                                                                    tCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGAATAATGCCGCGCGCGCGCGGTACAGACTGGCGGTTTcac                                                                  >  2:360438/1‑140 (MQ=255)                                                                     cGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTcaca                                                                 >  1:529516/1‑140 (MQ=255)                                                                     cGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTcaca                                                                 >  1:861771/1‑140 (MQ=255)                                                                        gtTTGATCCAGATGGTGCGATTCGCCATGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGTCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAata                                                              <  2:591504/140‑1 (MQ=255)                                                                          ttGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑Tg                                                           >  1:730269/1‑140 (MQ=255)                                                                           tGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGc                                                          >  1:48485/1‑140 (MQ=255)                                                                               ccAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCa                                                      <  2:638253/140‑1 (MQ=255)                                                                                   aTGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTTCTGCGCGCCCTCGGTACAGACTGGCAGTTTCACAAAA‑TGCGCCAgcgc                                                  >  2:269928/1‑140 (MQ=255)                                                                                       tGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCGCAATA‑TGCACCAGCGCAttt                                              >  1:119856/1‑140 (MQ=255)                                                                                       tGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACCAAT‑TTCACCACCGCAttt                                              >  2:512551/1‑140 (MQ=255)                                                                                            ttCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTtgtg                                         <  2:488330/140‑1 (MQ=255)                                                                                            ttCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTtgtg                                         <  2:861771/140‑1 (MQ=255)                                                                                                        tGCGCCAGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCg                             <  2:674425/140‑1 (MQ=255)                                                                                                        tGCGCCAGTGCCAGCGCGCGACCTAACGGTAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCg                             <  1:396929/140‑1 (MQ=255)                                                                                                             cAGTGCCAGCGCGCGCCGTACCGGGTACGCCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGc                        <  1:269928/140‑1 (MQ=255)                                                                                                              aGTGCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAAAATTCCCCCAGCG‑ATTTTTTTTGGGTTTAATGCGGTGGcc                       >  1:15620/1‑139 (MQ=255)                                                                                                                 gCCAGCGCGCGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAg                    <  1:124797/140‑1 (MQ=255)                                                                                                                     gcgcgcGACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCgtg                >  1:713791/1‑140 (MQ=255)                                                                                                                       gcgcgACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCGCAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTc              <  2:119856/140‑1 (MQ=255)                                                                                                                       gcgcgACGTACCGGGAACGGCTGATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTc              <  2:370884/140‑1 (MQ=255)                                                                                                                           gACGTACCGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGt          <  2:48485/140‑1 (MQ=255)                                                                                                                               tACCGGGAACGGCTTACCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCa      <  2:529516/140‑1 (MQ=255)                                                                                                                                 ccGGGAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGTTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAgt    <  2:862206/140‑1 (MQ=255)                                                                                                                                   gggAACGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAgtgt  <  1:274688/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATA‑TGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGT  >  NC_000913/862331‑862600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑