Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	886,100	T→C	D11G (GAT→GGT)	ybjI ←	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	886,100	0	T	C	100.0%	127.5 / NA	37	D11G (GAT→GGT)	ybjI	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

TCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCATCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCACT  >  NC_000913/885978‑886235                                                                                                                           |                                                                                                                                        tCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCaatt                                                                                                                        >  1:663022/1‑140 (MQ=255)       gATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAAttaattta                                                                                                                  >  1:620086/1‑140 (MQ=255)         taataTTGATTCCCGCTTGCGATTACAAAGCGAATTCCTAGTGCTTTCTTTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAAttaatttaat                                                                                                                <  2:310349/140‑1 (MQ=255)          aataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATg                                                                                                               >  1:700406/1‑140 (MQ=255)             atTGATTCCCGCTGGCGACTACGAATCGAATTCCTTGAGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCtc                                                                                                            <  1:312387/140‑1 (MQ=255)             atTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCtc                                                                                                            <  1:823014/140‑1 (MQ=255)               tGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCtcat                                                                                                          <  2:700406/140‑1 (MQ=255)                   tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAAc                                                                                                      >  1:793314/1‑140 (MQ=255)                      cGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAATAAAGTACCTCCCGTGTGTACCGCAAATAATTTAATGCTCATCAACTAt                                                                                                   >  2:312387/1‑140 (MQ=255)                      cGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTAt                                                                                                   >  2:730237/1‑140 (MQ=255)                               cTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCaa                                                                                                       >  1:123054/1‑127 (MQ=255)                               cTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCaa                                                                                                       <  2:123054/127‑1 (MQ=255)                                   aaaGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGt                                                                                      <  1:730237/140‑1 (MQ=255)                                    aaGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTc                                                                                     <  2:663022/140‑1 (MQ=255)                                                 gtgCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTAt                                                                        >  1:109733/1‑140 (MQ=255)                                                    cTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCgg                                                                     >  2:796692/1‑140 (MQ=255)                                                                     tGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTc                                                    <  2:620086/140‑1 (MQ=255)                                                                       aGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTcgc                                                  >  1:24192/1‑140 (MQ=255)                                                                       aGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTcgc                                                  >  1:603957/1‑140 (MQ=255)                                                                        gCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTcgcg                                                 >  1:692526/1‑140 (MQ=255)                                                                         ccATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTGTGCGTTTTGGTTTCGGGTTTGACGACGGCTTTCGCGa                                                >  1:253907/1‑140 (MQ=255)                                                                              aaCCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATa                                           <  2:109733/140‑1 (MQ=255)                                                                                  gCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCcg                                       <  2:793314/140‑1 (MQ=255)                                                                                  gCTCACGGTTATAGGTTATTTGTTCGCTTAACAAACTACCACCCATGTATACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCcg                                       <  2:246095/140‑1 (MQ=255)                                                                                   cTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCcgc                                      >  1:799856/1‑140 (MQ=255)                                                                                   cTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGCTTGTCGTCGGCTTGCGCGACAATAGCcgc                                      >  1:246095/1‑140 (MQ=255)                                                                                             taGGTTTTTTGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAAcc                            <  2:24192/140‑1 (MQ=255)                                                                                                  tttttGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATGGGGTTTGGCGACGGCTTTCGCGACAGTAGCCACGAGAATAACCAgcgc                       >  2:385189/1‑140 (MQ=255)                                                                                                    tttGATCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTGATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCa                     >  2:532966/1‑140 (MQ=255)                                                                                                        aTCGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTAc                 <  2:799856/140‑1 (MQ=255)                                                                                                          cGCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACaa               >  2:500008/1‑140 (MQ=255)                                                                                                           gCTTAAGAAAGTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAAc              <  1:532966/140‑1 (MQ=255)                                                                                                           gCTTAAGAAAGTACCACCCATGCCTACCGCAATTACTTTTATGCTCATAAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAAc              <  2:253907/140‑1 (MQ=255)                                                                                                                     gTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCa    <  2:585638/140‑1 (MQ=255)                                                                                                                     gTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCa    >  1:144993/1‑140 (MQ=255)                                                                                                                     gTACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCa    <  1:796692/140‑1 (MQ=255)                                                                                                                      tACCACCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCAc   >  2:61719/1‑140 (MQ=255)                                                                                                                       accaccCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCACt  >  2:142716/1‑140 (MQ=255)                                                                                                                       accaccCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCACt  >  2:832840/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                        TCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTTTTGATCGCTTAAGAAAGTACCATCCATGTCTACCGCAATTAATTTAATGCTCATCAACTATTCTCCATCGCCGTCTGCGTTTTGGTATCGGGTTTGGCGACGGCTTTCGCGACAATAGCCGCGAGAATAACCAGCGCCAGTACAACCAGCATTGCACT  >  NC_000913/885978‑886235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑