Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	929,457	A→G	N502N (AAT→AAC)	cydD ←	glutathione/L‑cysteine ABC exporter subunit CydD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	929,457	0	A	G	100.0%	175.1 / NA	51	N502N (AAT→AAC)	cydD	glutathione/L‑cysteine ABC exporter subunit CydD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/20);  total (31/20)

GCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGATTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGC  >  NC_000913/929320‑929591                                                                                                                                          |                                                                                                                                       gCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGtt                                                                                                                                      <  2:482870/140‑1 (MQ=255)          tctAACTGGTGGGTGACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCCGGCAGCGGGTTCATCCAACAGTAATTGCGACCAGGGGTTTAGtctgtc                                                                                                                             >  2:358092/1‑135 (MQ=255)           ctACCTGGTGGGGGACCATGAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            <  2:650060/140‑1 (MQ=255)           ctAACTGGTGGGTGCCCATTAATGTGGTCTGGCGCAGCGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            <  1:423498/140‑1 (MQ=255)                tggtggGTGACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGCGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGc                                                                                                                       >  2:657059/1‑140 (MQ=255)                 ggtgggtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGcc                                                                                                                      >  2:84699/1‑140 (MQ=255)                   tgggtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            <  2:812005/132‑1 (MQ=255)                   tgggtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            <  2:451844/132‑1 (MQ=255)                   tgggtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            >  1:812005/1‑132 (MQ=255)                   tgggtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCa                                                                                                                            >  1:451844/1‑132 (MQ=255)                      gtgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGGTTAGTAACGCACGGGCCACCGc                                                                                                                 >  1:416292/1‑140 (MQ=255)                       tgACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGcc                                                                                                                <  1:134180/140‑1 (MQ=255)                             ttAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAAAAGTAATAGAGAATAGGAGTTTAGTAGCGCATGGGCCACCGCCACgcgc                                                                                                          >  2:347579/1‑140 (MQ=255)                             ttAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGCTCATCCAACAGGAATAGCGAACAGGGGGTTAATAGCGCACGGGCCACCGCCACgcgc                                                                                                          >  1:309079/1‑140 (MQ=255)                                     gTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTg                                                                                                  >  1:586305/1‑140 (MQ=255)                                     gTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTg                                                                                                  >  1:49849/1‑140 (MQ=255)                                     gTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAAAGCAAGGCCCACCGCCACGCGCTGCGCCTg                                                                                                  >  1:336201/1‑140 (MQ=255)                                     gTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGGTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGATTAGTAAGGCAGGGGCCACCGCCACGCGCTGCGCCTg                                                                                                  >  1:372384/1‑140 (MQ=255)                                        tGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGccc                                                                                               <  1:569508/140‑1 (MQ=255)                                            gcAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACg                                                                                           <  2:49849/140‑1 (MQ=255)                                                     cggcATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCgg                                                                                  >  1:798542/1‑140 (MQ=255)                                                      ggcATTCGGCGCCACCTTTACGGGCTGTTCAATCTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACATTAATAGCGAACAGGGGTTTAGAAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCggg                                                                                 <  2:372384/140‑1 (MQ=255)                                                                  cTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGGTGCGCCTGCCCCACCGAAAGGCGGGCAGCCTGGTCGc                                                                     >  1:346016/1‑140 (MQ=255)                                                                    ccATTACGCGCTGTTCTCTGTGAGCATAAAAACTGCCAGCGGGTTCATGCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCa                                                                   <  2:416292/140‑1 (MQ=255)                                                                    ccATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGTGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCa                                                                   >  2:773154/1‑140 (MQ=255)                                                                    ccATTACGCGCTGTTCAATGTGAGCATCAAGGCTGTCAGTGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGGCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCa                                                                   <  2:309079/140‑1 (MQ=255)                                                                     cATTACGCGCTGTTCCCTGTGACCACCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCaa                                                                  <  1:341417/140‑1 (MQ=255)                                                                         aCGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAgg                                                              <  2:427933/140‑1 (MQ=255)                                                                         aCGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAgg                                                              >  1:427933/1‑140 (MQ=255)                                                                             gcTGTTCACTGTGAGCACCAAGTCTGGCAGCCGTTTCATCCAACAGTGTTAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTa                                                          <  2:398111/140‑1 (MQ=255)                                                                              cTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTAt                                                         >  1:47021/1‑140 (MQ=255)                                                                                gTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCa                                                       >  1:500412/1‑140 (MQ=255)                                                                                         gAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTtgt                                              >  1:521569/1‑140 (MQ=255)                                                                                          aGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCTCGGAAAGGCGGGGAGCATGGTCGCCAACAGGCGTATCAACGCCTtgtg                                             >  2:258166/1‑140 (MQ=255)                                                                                           gCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTgg                                            >  1:574632/1‑140 (MQ=255)                                                                                              tCAAGGCTGGCAGTGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGggag                                         <  1:773154/140‑1 (MQ=255)                                                                                              tCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGggag                                         >  1:462027/1‑140 (MQ=255)                                                                                                  ggCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGc                                     >  2:483658/1‑140 (MQ=255)                                                                                                    cTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCgg                                   <  1:485571/140‑1 (MQ=255)                                                                                                    cTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCgg                                   >  2:123869/1‑140 (MQ=255)                                                                                                      ggCAGCGGGCTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTa                                 >  2:649904/1‑140 (MQ=255)                                                                                                       gCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAg                                >  1:750297/1‑140 (MQ=255)                                                                                                                tCATCCAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCt                       <  2:521569/140‑1 (MQ=255)                                                                                                                     cAACAGTAATAGCGAACAGGGGTTTAGTAACGCACTGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGAccc                  <  2:336201/140‑1 (MQ=255)                                                                                                                     cAACAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGAccc                  <  1:483658/140‑1 (MQ=255)                                                                                                                        cAGTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAgg               >  2:864175/1‑140 (MQ=255)                                                                                                                          gTAATAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCg             <  1:123869/140‑1 (MQ=255)                                                                                                                          gTAATAGCGAACAGGGGTTTAGAAACGCACGTGCCTCCGTCACGCGCTACGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCg             <  2:346016/140‑1 (MQ=255)                                                                                                                             aTAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTATGGGAGGGGCGATATAAACTCTCTGACCCAGGCGTTa          >  1:402716/1‑140 (MQ=255)                                                                                                                             aTAGCGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTACCCAACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTa          >  2:187921/1‑140 (MQ=255)                                                                                                                                 cGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCATCGCCTTGTCGGAGGAGAGGTAGAAACTCGCGGACCCAGGCGTTATCCa      >  2:259057/1‑140 (MQ=255)                                                                                                                                 cGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAGCGCCTTGTCGGAGGAGCGGTAGAAACTCGCCGACCCAGGCGTTATCCa      >  2:262985/1‑140 (MQ=255)                                                                                                                                 cGAACAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCa      >  1:752216/1‑140 (MQ=255)                                                                                                                                    aCAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAgcg   <  2:574632/140‑1 (MQ=255)                                                                                                                                     cAGGGGTTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAgcgc  >  1:71606/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTGGCGCAGAGAGGCGGCATTCAGCGCCTCCATTACGCGCTGTTCACTGTGAGCATCAAGGCTGGCAGCGGGTTCATCCAACAGTAATAGCGAACAGGGATTTAGTAACGCACGGGCCACCGCCACGCGCTGCGCCTGCCCCACGGAAAGGCGGGCAGCCTGGTCGCCAACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGC  >  NC_000913/929320‑929591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑