Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,725	A→G	L254L (TTA→TTG)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,725	0	A	G	100.0%	179.8 / NA	51	L254L (TTA→TTG)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/22);  total (29/22)

CCACGACCGTTCGTTTATCCGCAATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCG  >  NC_000913/1010593‑1010861                                                                                                                                     |                                                                                                                                         ccACGACCGTTCGTTTATCCGCAATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATg                                                                                                                                   >  2:180117/1‑140 (MQ=255)   aCGACCGTTCGTTTATCCGCAATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGcc                                                                                                                                 >  2:578112/1‑140 (MQ=255)                        atatGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGgc                                                                                                             >  2:728088/1‑139 (MQ=255)                        atatGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGgc                                                                                                             <  1:728088/139‑1 (MQ=255)                            ggCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGgc                                                                                                             <  1:15225/135‑1 (MQ=255)                            ggCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGgc                                                                                                             >  2:15225/1‑135 (MQ=255)                            ggCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAgg                                                                                                        <  1:120590/140‑1 (MQ=255)                             gCGACGCGCATTGTTGAGCTCGATCGCGGCATGCTGGTGCCCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGa                                                                                                       <  2:328416/140‑1 (MQ=255)                              cGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGaa                                                                                                      <  1:70410/140‑1 (MQ=255)                              cGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGaa                                                                                                      <  1:578112/140‑1 (MQ=255)                                aCGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGGAAAATGCTGAGGTCGATCGCAGACTGGCGCAGGAaga                                                                                                    >  1:328416/1‑140 (MQ=255)                                 cgcgCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAagag                                                                                                   >  2:777215/1‑140 (MQ=255)                                   cgcATTCTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAACATGCCGAATTCGATCGCAAACTGGCGCAGGAAGAGgt                                                                                                 >  1:287982/1‑140 (MQ=255)                                    gcATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGgtg                                                                                                <  1:777215/140‑1 (MQ=255)                                        tgttgATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGa                                                                                            <  1:820754/140‑1 (MQ=255)                                             aTCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGc                                                                                       >  2:427230/1‑140 (MQ=255)                                               ctcGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCa                                                                                     >  1:101691/1‑140 (MQ=255)                                                  gATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAggg                                                                                  >  1:463330/1‑140 (MQ=255)                                                  gATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAg                                                                                    >  1:44950/1‑138 (MQ=255)                                                  gATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAg                                                                                    <  2:44950/138‑1 (MQ=255)                                                    tCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGa                                                                                >  1:765675/1‑140 (MQ=255)                                                              cTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGTGGATCAAAGCACg                                                                      >  2:159698/1‑140 (MQ=255)                                                                  gcaCCTATCCAGGGAATTACGACCAGTCCCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt                                                                  <  2:109879/138‑1 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCg                                                                   <  2:4468/122‑1 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCg                                                                   >  1:4468/1‑122 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:797649/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:524849/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:825142/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  2:471080/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:561536/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:683876/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:22866/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                 >  2:441463/1‑140 (MQ=255)                                                                                   tACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAACCACGCCGTACCCGTAATGAAGGCcg                                                 >  1:384762/1‑140 (MQ=255)                                                                                       aCCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTa                                             <  2:101691/140‑1 (MQ=255)                                                                                         cAGTACCTGCTGGAAAACGAAGCAGCCCTGCACGTAGAAAAATTGCAATATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAcg                                           <  2:287982/140‑1 (MQ=255)                                                                                            tACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACgcgc                                        <  2:765675/140‑1 (MQ=255)                                                                                              ccTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGccc                                      <  1:159698/140‑1 (MQ=255)                                                                                                      aaaaaGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCg                              >  2:578073/1‑140 (MQ=255)                                                                                                      aaaaaGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCg                              >  2:7188/1‑140 (MQ=255)                                                                                                       aaaaGAAGAAGCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGa                             >  1:182296/1‑140 (MQ=255)                                                                                                            aagaagCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGt                        <  1:578073/140‑1 (MQ=255)                                                                                                              gacgCCCGGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTGCGCGCCCTGAAAGCGATGCGTcg                      <  2:124181/137‑1 (MQ=255)                                                                                                              gaagCCCTGCGCGTGGGTGAATTGCAAAATGACGACTTCTATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcg                      <  2:384762/140‑1 (MQ=255)                                                                                                              gaagCCCCGCGAGTGGACAAATGGCAAAATGTCAATTTCTGTCGCAATCTGGCGCAGGAAGAGGTTTGGCCCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcg                      <  2:271481/140‑1 (MQ=255)                                                                                                               aagCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgc                     >  2:613568/1‑140 (MQ=255)                                                                                                                 gCCCTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGa                   <  1:471080/140‑1 (MQ=255)                                                                                                                   ccTGCGCGTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATAAAGACCGAGTACGCGCGCTGAGAGCGATGCGTCGCGAAc                 >  2:248438/1‑140 (MQ=255)                                                                                                                          gTGGAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa          <  2:22866/140‑1 (MQ=255)                                                                                                                            ggAAGAATTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAAc        >  2:546762/1‑140 (MQ=255)                                                                                                                                  aTTGCAAAATGCCGATTTCGATCGCAAACAGGCGCAGCAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTcgcg  <  1:248438/140‑1 (MQ=255)                                                                                                                                  aTTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTcgcg  <  2:561536/140‑1 (MQ=255)                                                                                                                                  aTTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTcgcg  <  2:524849/140‑1 (MQ=255)                                                                                                                                  aTTGCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTcgcg  <  1:613568/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CCACGACCGTTCGTTTATCCGCAATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCG  >  NC_000913/1010593‑1010861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑