Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	120,112	A→G	T278A (ACG→GCG)	ampE →	protein AmpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	120,112	0	A	G	100.0%	149.1 / NA	43	T278A (ACG→GCG)	ampE	protein AmpE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/26);  total (17/26)

GTTAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCA  >  NC_000913/119985‑120246                                                                                                                                |                                                                                                                                       gtTAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACgggg                                                                                                                            <  2:194302/140‑1 (MQ=255)   tAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAGCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCg                                                                                                                          <  1:29599/140‑1 (MQ=255)   tAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCg                                                                                                                          >  1:438291/1‑140 (MQ=255)    aaCGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGt                                                                                                                         <  2:154157/140‑1 (MQ=255)    aaCGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGt                                                                                                                         <  2:171358/140‑1 (MQ=255)     aCGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGtt                                                                                                                        >  1:498789/1‑140 (MQ=255)         gTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTTGTGGTCGTAGTGGTGAGTGCACTACGGGCGCTTTACGGGGCGTTGgtg                                                                                                                    >  2:224404/1‑140 (MQ=255)         gTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGgtg                                                                                                                    >  1:633349/1‑140 (MQ=255)          tCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGgtgt                                                                                                                   <  1:513720/140‑1 (MQ=255)          tCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGgtgt                                                                                                                   >  1:591054/1‑140 (MQ=255)          tCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTAGTGATTGCACTACTGGCGATTAACGGGGCGTTGgtgt                                                                                                                   >  1:422835/1‑140 (MQ=255)             ggCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTaaa                                                                                                                <  2:437400/140‑1 (MQ=255)                    ttCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGattatt                                                                                                         >  2:799491/1‑140 (MQ=255)                    ttCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGattatt                                                                                                         >  2:142410/1‑140 (MQ=255)                       tctctGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGcc                                                                                                      <  1:711214/140‑1 (MQ=255)                       tctctGGCGCGTGAACCGCATGCCGCTAAGGTGGAGAAGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGcc                                                                                                      <  1:224404/140‑1 (MQ=255)                           tGGCGCGTTAACCGCACTTCGATAAGGTGGGGAGGTCGAGGGCAGCGGGTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCa                                                                                                  <  2:422835/140‑1 (MQ=255)                           tGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCa                                                                                                  <  1:658216/140‑1 (MQ=255)                           tGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCa                                                                                                  <  1:687091/140‑1 (MQ=255)                           tGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCa                                                                                                  <  1:498055/140‑1 (MQ=255)                           tGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCa                                                                                                  <  2:633349/140‑1 (MQ=255)                             gcgcGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCAcc                                                                                                <  2:32125/140‑1 (MQ=255)                             gcgcGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCAcc                                                                                                <  1:142410/140‑1 (MQ=255)                              cgcgTGAACCGCATGTCGATAAGGTGGAGATGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCAccc                                                                                               >  2:199960/1‑140 (MQ=255)                              cgcgTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCAccc                                                                                               >  2:543208/1‑140 (MQ=255)                                       cGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGt                                                                                      <  2:77488/140‑1 (MQ=255)                                        gCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTg                                                                                     <  1:799491/140‑1 (MQ=255)                                                  aaGGTGGAGACGCCGAATGCAGCGGTTTCAAGGGCTAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga                                                                           <  2:344120/140‑1 (MQ=255)                                                     gcggAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                        >  2:524992/3‑140 (MQ=255)                                                        gagaCGCCGAAGGCAGCTGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAtt                                                                     >  1:558865/1‑140 (MQ=255)                                                           aCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                  >  1:645906/1‑140 (MQ=255)                                                              ccGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcg                                                               <  1:199960/140‑1 (MQ=255)                                                                                   gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                          <  1:524992/140‑1 (MQ=255)                                                                                           aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                  <  2:645906/140‑1 (MQ=255)                                                                                                cgttcgtGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAg                             >  1:812076/1‑140 (MQ=255)                                                                                                  ttcgtGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                            >  1:47455/1‑139 (MQ=255)                                                                                                  ttcgtGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                            <  2:47455/139‑1 (MQ=255)                                                                                                       ggtcgtggtGGTGATTGAACTATTGGCGATTTACGTGGCCTTGGTGTACAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                      <  2:270074/140‑1 (MQ=255)                                                                                                        gtcgtggtGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                     >  2:109594/1‑140 (MQ=255)                                                                                                                 gtgATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca            <  2:498789/140‑1 (MQ=255)                                                                                                                 gtgATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca            <  1:109594/140‑1 (MQ=255)                                                                                                                        cACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg     <  2:438291/140‑1 (MQ=255)                                                                                                                           tactGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCa  >  2:114154/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       GTTAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCA  >  NC_000913/119985‑120246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑