Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,016,702	A→G	V533A (GTG→GCG)	ycbZ ←	putative ATP‑dependent protease YcbZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,016,702	0	A	G	100.0%	158.2 / NA	46	V533A (GTG→GCG)	ycbZ	putative ATP‑dependent protease YcbZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

TAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCACATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCA  >  NC_000913/1016569‑1016835                                                                                                                                      |                                                                                                                                      tAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGt                                                                                                                                 <  1:184454/140‑1 (MQ=255)  aaTGGCCATTGAATCCGGTGACGTCTTCCCTGTTGCGATGCCTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGAATTAATAATAACGGCAGTGCGTCAGTCGCCTCGTc                                                                                                                                <  2:217618/140‑1 (MQ=255)     ggCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTAc                                                                                                                             <  1:198892/140‑1 (MQ=255)         aTGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGcc                                                                                                                         >  1:148520/1‑140 (MQ=255)          tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGCTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGccc                                                                                                                        >  1:217618/1‑140 (MQ=255)                aCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATgg                                                                                                                  >  2:175905/1‑140 (MQ=255)                         tccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCATTCGCATCGTCCACTGAACAGATGGCGAATTTGc                                                                                                         >  2:390144/1‑140 (MQ=255)                                  ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTa                                                                                                <  1:175905/140‑1 (MQ=255)                                  ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTa                                                                                                <  1:479075/140‑1 (MQ=255)                                       aTGCTTGCGCGATACGTTCCTGCATGGTTTGCATCTGCGACGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCt                                                                                           <  2:230207/140‑1 (MQ=255)                                         gCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCttt                                                                                         <  1:605285/140‑1 (MQ=255)                                         gCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCttt                                                                                         <  2:612343/140‑1 (MQ=255)                                          cTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTc                                                                                        >  2:755827/1‑140 (MQ=255)                                           ttGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCa                                                                                       <  2:148433/140‑1 (MQ=255)                                            tGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCa                                                                                       <  2:128374/139‑1 (MQ=255)                                            tGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCa                                                                                       >  1:128374/1‑139 (MQ=255)                                            tGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCAc                                                                                      <  2:573710/140‑1 (MQ=255)                                            tGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTAGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCAc                                                                                      >  2:514284/1‑140 (MQ=255)                                            tGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTAGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCAc                                                                                      <  1:514284/140‑1 (MQ=255)                                              cgcgATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCTCAGATGGGGAATTTACCTTCTGCTACCGCTTTCACCa                                                                                    >  1:230207/1‑140 (MQ=255)                                              cgcgATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCa                                                                                    <  2:116930/140‑1 (MQ=255)                                                  aTACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTc                                                                                <  2:5465/140‑1 (MQ=255)                                                           tGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAg                                                                       >  1:579180/1‑140 (MQ=255)                                                             gATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGAc                                                                     >  2:865666/1‑140 (MQ=255)                                                               tGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACtt                                                                   >  1:665906/1‑140 (MQ=255)                                                                ggTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTa                                                                  >  1:507305/1‑140 (MQ=255)                                                                    tGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATg                                                              >  1:181387/1‑140 (MQ=255)                                                                      cATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATATGCCTTCTGCTACCGCATTCACCAGGTCACTGTGAAGACTTAAATGGc                                                            >  2:315002/1‑140 (MQ=255)                                                                       aTCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCGGTTCACTGTCAAGACTTAAATGGCg                                                           >  1:393936/1‑140 (MQ=255)                                                                       aTCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCg                                                           <  1:606061/140‑1 (MQ=255)                                                                            cgtcgtTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGt                                                      >  2:708217/1‑140 (MQ=255)                                                                                   tGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCg                                                           <  2:157745/128‑1 (MQ=255)                                                                                   tGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCg                                                           >  1:157745/1‑128 (MQ=255)                                                                                    ggCCTTCGCCATCCCACACCAGGTTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCtgtg                                              <  1:390144/140‑1 (MQ=255)                                                                                    ggCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCtgtg                                              <  2:507305/140‑1 (MQ=255)                                                                                         tCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCAACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGAt                                         >  1:806727/1‑140 (MQ=255)                                                                                          cGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAg                                                   >  1:680254/1‑129 (MQ=255)                                                                                          cGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAg                                                   <  2:680254/129‑1 (MQ=255)                                                                                               tCCCACACCAGATTTGATAATAACGGCAGGGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGac                                   <  1:353557/140‑1 (MQ=255)                                                                                                    cacCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCATTGTGAAGATTTATAGGGCGAACGTTAGCTGGGGGGATAACGACACCtt                              >  2:407627/1‑140 (MQ=255)                                                                                                     acCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTggg                                            <  1:683187/125‑1 (MQ=255)                                                                                                     acCAGATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTggg                                            >  2:683187/1‑125 (MQ=255)                                                                                                         gATTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTc                         >  2:216143/1‑140 (MQ=255)                                                                                                          aTTTAATAATAACGGTAGTGCGACACTCGGATCGTCTCCTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTcc                        <  1:407627/140‑1 (MQ=255)                                                                                                          aTTTAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTCAGCAGTCGGGATACTGACACCTTGTTTcc                        >  1:404495/1‑140 (MQ=255)                                                                                                           tttAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTccc                       <  1:755827/140‑1 (MQ=255)                                                                                                           tttAATAATAACGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTccc                       >  1:752207/1‑140 (MQ=255)                                                                                                               ataataaCGGCAGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGtt                   <  1:708217/140‑1 (MQ=255)                                                                                                                          aGTGCGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTg        <  2:799575/140‑1 (MQ=255)                                                                                                                              cGTCAGTCGCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTgg    >  1:176122/1‑140 (MQ=255)                                                                                                                                tcagtcgCATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCa  <  2:579180/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAATAACGGCAGTGCGTCAGTCACATCGTCTACTGCCCAGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCA  >  NC_000913/1016569‑1016835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑