Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,045,000 C→T T268T (ACG→ACA)  gfcE ← putative exopolysaccharide export lipoprotein GfcE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,045,0000CT98.0% 165.8 / NA 50T268T (ACG→ACA) gfcEputative exopolysaccharide export lipoprotein GfcE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/25);  minor base G (0/1);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCATAGAGGTTCAC  >  NC_000913/1044864‑1045134
                                                                                                                                        |                                                                                                                                      
tGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGtgtg                                                                                                                                     <  2:701743/140‑1 (MQ=255)
 ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGc                                                                                                                                    <  1:486741/140‑1 (MQ=255)
 ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGc                                                                                                                                    <  1:67754/140‑1 (MQ=255)
  gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGct                                                                                                                                   >  1:435818/1‑140 (MQ=255)
  gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGct                                                                                                                                   >  1:88474/1‑140 (MQ=255)
          gCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTAc                                                                                                                           <  2:585438/140‑1 (MQ=255)
                   cTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCAc                                                                                                                  >  2:434283/1‑140 (MQ=255)
                      gTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCTTACCGCTGCGGTCCATTTTCAGTGTACTCTGTTTACCCACTTCACCCa                                                                                                               >  2:277290/1‑140 (MQ=255)
                            cGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGa                                                                                                         <  1:97772/140‑1 (MQ=255)
                             gTTGCACGAATAACACAAATCCCCGCCGCTTCAGCGAGATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                                                        <  1:255318/140‑1 (MQ=255)
                             gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                                                        <  2:13947/140‑1 (MQ=255)
                             gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                                                        >  1:660860/1‑140 (MQ=255)
                             gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACACCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                                                        <  2:329339/140‑1 (MQ=255)
                                 cACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACt                                                                                                    >  1:586075/1‑140 (MQ=255)
                                 cACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACt                                                                                                    >  1:726784/1‑140 (MQ=255)
                                   cGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACttt                                                                                                  <  1:557237/140‑1 (MQ=255)
                                               aTCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGCGCTCTGTTTACCGACTACACCCATCGCGAAAACTTTCAGATCATCGtt                                                                                      >  2:313506/1‑140 (MQ=255)
                                                     gTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACttt                                                                                                  <  2:597051/122‑1 (MQ=255)
                                                     gTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACttt                                                                                                  >  1:597051/1‑122 (MQ=255)
                                                     gTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCgg                                                                                <  2:175496/140‑1 (MQ=255)
                                                      tCGCATCAGCGACATCCTGATTCATTCCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGa                                                                               <  1:791342/140‑1 (MQ=255)
                                                      tCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGa                                                                               <  2:88474/140‑1 (MQ=255)
                                                      tCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGa                                                                               <  2:726784/140‑1 (MQ=255)
                                                                         aTTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCg                                                            >  1:727170/1‑140 (MQ=255)
                                                                              tACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgc                                                       >  2:57695/1‑140 (MQ=255)
                                                                               aCCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgca                                                      <  1:450121/140‑1 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGccc                                                             >  1:844111/1‑129 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGccc                                                             <  2:844111/129‑1 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGcagcag                                                  >  2:611379/1‑140 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGcagcag                                                  >  1:715932/1‑140 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGcagcag                                                  >  2:760943/1‑140 (MQ=255)
                                                                                   tCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGCTGCGCGGCATAAATTAAATATCGCCCGGGTGcagcag                                                  >  1:389052/1‑140 (MQ=255)
                                                                                     aGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                                                >  1:484474/1‑140 (MQ=255)
                                                                                     aGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                                                >  2:60231/1‑140 (MQ=255)
                                                                                     aGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                                                >  1:590267/1‑140 (MQ=255)
                                                                                     aGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                                                >  1:217985/1‑140 (MQ=255)
                                                                                     aGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTACGCGGAATAAATAAAATATCGACCGGGTGCAGCAGtt                                                >  1:352827/1‑140 (MQ=255)
                                                                                               aGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGgcg                                      <  2:528215/140‑1 (MQ=255)
                                                                                                         ccAGGGTCATACCGCTGGGGTCCATTTTCAGTGTGGTCTGTTTACCCAGTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCa                            <  2:352827/140‑1 (MQ=255)
                                                                                                           aGGGTCATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACg                          <  2:435818/140‑1 (MQ=255)
                                                                                                                cATACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCa                     <  2:586075/140‑1 (MQ=255)
                                                                                                                 aTACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCAt                    <  2:575138/140‑1 (MQ=255)
                                                                                                                 aTACCGCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCAt                    <  1:719493/140‑1 (MQ=255)
                                                                                                                  tACCGCTGCGGTCCATTATCAGGGTGCTCTGTTTACCCACTTCACCCAACACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATc                   <  2:389052/140‑1 (MQ=255)
                                                                                                                      gCTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAgcg               <  2:660860/140‑1 (MQ=255)
                                                                                                                       cTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAgcg               >  2:601530/1‑139 (MQ=255)
                                                                                                                       cTGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAgcg               <  1:601530/139‑1 (MQ=255)
                                                                                                                        tGCGGTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCa             <  1:760943/140‑1 (MQ=255)
                                                                                                                            gTCCATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCATaga         >  2:568995/1‑140 (MQ=255)
                                                                                                                               cATTTTCAGTGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCATAGAGGt      >  2:769653/1‑140 (MQ=255)
                                                                                                                                   ttCAGTGTGCTCTGTATACCCACTTGACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCATAGAGGTTCAc  <  1:313506/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGCCACGCTGCATCAGCGCATAGAGGTTCAC  >  NC_000913/1044864‑1045134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑