Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,048,903	T→C	T144A (ACT→GCT)	gfcB ←	lipoprotein GfcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,048,903	0	T	C	100.0%	168.4 / NA	48	T144A (ACT→GCT)	gfcB	lipoprotein GfcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

GTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATT  >  NC_000913/1048770‑1049038                                                                                                                                      |                                                                                                                                        gTAGCCGAATCGACCCAGAATGTGTTTTGCCGCTGGTTTCCTGGCCGCTTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACt                                                                                                                                   <  2:330916/140‑1 (MQ=255) gTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACt                                                                                                                                   <  2:162771/140‑1 (MQ=255)  tAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGTCCCGCAATATTCCGCGACTCTAATCTAGCAAAGGAAAAAGCAGAACTc                                                                                                                                  >  2:316430/1‑140 (MQ=255)  tAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTc                                                                                                                                  >  2:862723/1‑140 (MQ=255)       gAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAATCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGt                                                                                                                             >  1:741644/1‑140 (MQ=255)                 aGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGa                                                                                                                   <  2:786357/140‑1 (MQ=255)                 aGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGa                                                                                                                   <  2:200566/140‑1 (MQ=255)                      gtgtTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATg                                                                                                              <  1:751603/140‑1 (MQ=255)                        gtTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGt                                                                                                            <  1:38865/140‑1 (MQ=255)                             gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCTGCAAAGGAAAAAACCGTACTCAGGGACGCAGAGCGAAAATGGTTGTCt                                                                                                       >  2:406413/1‑140 (MQ=255)                                 cTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCtt                                                                                                      >  2:177384/1‑137 (MQ=255)                                 cTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCtt                                                                                                      <  1:177384/137‑1 (MQ=255)                                  tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                  <  1:862723/140‑1 (MQ=255)                                  tGTTTTTCTGGCCGCGTGGAAGGCAGCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                  <  1:291952/140‑1 (MQ=255)                                      tttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCag                                                                                              <  1:499361/140‑1 (MQ=255)                                            gCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAata                                                                                        >  2:71892/1‑140 (MQ=255)                                                cgTGGAAGTCACCTCCTCCTGCTAGACATCACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATcgc                                                                                    <  1:316430/140‑1 (MQ=255)                                                 gTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATcgcg                                                                                   <  1:83408/140‑1 (MQ=255)                                                                  cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                  <  1:778991/140‑1 (MQ=255)                                                                         aCATTACACAGCACATTGCGACCCTCAATATTCTCCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAACGGTTGTCTCCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTAt                                                           >  2:136177/1‑140 (MQ=255)                                                                         aCATTACACAGCACAATGCGACACGTAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCATCCAGCGGCTGCCATCTTTTAt                                                           <  1:294091/140‑1 (MQ=255)                                                                              acacAGCACATTGCGACCCGCAATATTCAGAGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGcc                                                                    <  1:844220/126‑1 (MQ=255)                                                                              acacAGCACATTGCGACCCGCAATATTCAGAGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGcc                                                                    >  2:844220/1‑126 (MQ=255)                                                                                  aGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAgcgc                                                  >  2:688289/1‑140 (MQ=255)                                                                                    cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAgc                                                    >  2:68908/1‑136 (MQ=255)                                                                                    cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAgc                                                    <  1:68908/136‑1 (MQ=255)                                                                                     acaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGtt                                               >  2:806388/1‑140 (MQ=255)                                                                                       aTTGCGACCCGCAATCTTCAGCATCGCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                             <  1:406413/140‑1 (MQ=255)                                                                                              cccGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATTGCGCGTCCGGCGACTGTCATCTTTTTTTGCTAGCGCGTTACGCAGAgg                                      >  2:226865/1‑140 (MQ=255)                                                                                              cccGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAgg                                      >  1:668588/1‑140 (MQ=255)                                                                                                cGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGTGCGTCCAGCGGCGGGCTTCTTTTATGGCCAGCGCGTTACGCAGAGGGt                                    >  2:371736/1‑140 (MQ=255)                                                                                                  cAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCACAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                   >  1:475151/1‑139 (MQ=255)                                                                                                  cAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCACAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                   <  2:475151/139‑1 (MQ=255)                                                                                                        tCAGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCg                            >  1:560053/1‑140 (MQ=255)                                                                                                          aGCGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGa                          >  2:204870/1‑140 (MQ=255)                                                                                                            cGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGcc                              <  2:784262/134‑1 (MQ=255)                                                                                                            cGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGcc                              >  1:784262/1‑134 (MQ=255)                                                                                                            cGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAAt                        >  2:580728/1‑140 (MQ=255)                                                                                                            cGTCTCTAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTAATTGCCAACGCATTACGCAGAGGGTCCTGCCCGGAAt                        >  1:308695/1‑140 (MQ=255)                                                                                                               ctctAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTg                     >  2:692035/1‑140 (MQ=255)                                                                                                               ctctAATCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATCGCCAGCGCGTTACGAAGAGGGCCCTGCCCGGAATTTg                     >  1:420799/1‑140 (MQ=255)                                                                                                                    aTCCAGCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACt                <  1:71892/140‑1 (MQ=255)                                                                                                                         gCAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag           <  2:48404/140‑1 (MQ=255)                                                                                                                         gCAAAGGAAAAAGCAGAACTCAAGGTCGCAGAGCGTAAACGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag           <  2:308695/140‑1 (MQ=255)                                                                                                                          cAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTATGCAAAGGGTCCTCCCCGGAATTTGTCCCTTCcagc          >  2:407160/1‑140 (MQ=255)                                                                                                                          cAAAGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagc          >  1:687733/1‑140 (MQ=255)                                                                                                                             aGGAAAAAGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGa       <  1:688289/140‑1 (MQ=255)                                                                                                                                aaaaaGCAGAACTCAAGGTCGCCGATCGACAATGGTTTTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGatta    <  1:226865/140‑1 (MQ=255)                                                                                                                                  aaaGCAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGattatt  >  2:682277/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATT  >  NC_000913/1048770‑1049038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑