Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,057,108	A→G	E283G (GAA→GGA)	torT →	periplasmic trimethylamine‑N‑oxide binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,057,108	0	A	G	100.0%	56.9 / NA	18	E283G (GAA→GGA)	torT	periplasmic trimethylamine‑N‑oxide binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

GCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGAACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCGCTGTCACCAGGG  >  NC_000913/1056972‑1057238                                                                                                                                         |                                                                                                                                   gcggcACTGGCGGAAGGGCGTACCCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCGCATCAGGTGTCTCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACt                                                                                                                                 <  2:843085/140‑1 (MQ=255) gcggcAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCCGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCACCGATCAAATGGTCTGGCAGGGGGGAcc                                                                                                                                 >  2:604065/1‑139 (MQ=255)   ggcAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTgg                                                                                                                               <  2:205580/140‑1 (MQ=255)           gggAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGttttg                                                                                                                       >  2:472750/1‑137 (MQ=255)                     tAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCa                                                                                                             <  1:200336/140‑1 (MQ=255)                                       tACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAAt                                                                                           <  2:86799/140‑1 (MQ=255)                                                    ttttATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATaa                                                                              >  1:148063/1‑140 (MQ=255)                                                                     ggTGTATCGCGGGCTGAAGCGGTGAAGAGTGATTATGGCTGCCAGCGAACAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGAttt                                                             <  2:349289/140‑1 (MQ=255)                                                                     ggTGTATCGCGGGCTGAAGCGGGGAAGAGCGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGCTGTGCAGGCCCACAAGGAATTATAGGGGGCATCGGTGTTTTATAAAGTCCGCCCCCCGGttt                                                             >  2:141012/1‑140 (MQ=255)                                                                         tATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGAAATCGGTATTTCATAAAGTCACCCCCCCCATTTTTGt                                                         >  1:335157/1‑140 (MQ=255)                                                                                ggCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACg                                                  <  1:834742/140‑1 (MQ=255)                                                                                 gCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGGTGAGCAGGGCATCAGGCAATTACAGGGGGAATCGGGTTCTGATAATGTCAGCCCCCCGATTTTTGTTCTGACGc                                                 >  2:436368/1‑140 (MQ=255)                                                                                                 gTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCg                                 <  2:162041/140‑1 (MQ=255)                                                                                                     ttATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCCTCCGGCAAATACAGGGGCAATCGGGTTTTTGTAATGTCAGCCCCCCCATTTTAGTTTTGACGCCGCAAAAAGCCGACCGCGaa                             >  2:730352/1‑140 (MQ=255)                                                                                                              ccAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGGAGGCCATCAGGGAATTAAAGGGGGAATCGGTTTCTGGTAATGTCACCCCACCGATTTTTGTTTTGACGCCGCAAAAAGCCGACCGTGAAAAAATTtgc                    >  2:129867/1‑140 (MQ=255)                                                                                                                 gCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTcgccgc                 <  1:747834/140‑1 (MQ=255)                                                                                                                 gCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTcgccgc                 <  1:169308/140‑1 (MQ=255)                                                                                                                            gTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGGCATCGGTTTCTGATAATGTCAGCCCACCGGTTTTTATTCTGACGCCGCAAAATGCCGACCGCGAACATATTTGGCCCACGCTGTCAcc      >  2:446188/1‑140 (MQ=255)                                                                                                                                ggCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCGCTGTCACCAggg  <  1:604065/140‑1 (MQ=255)                                                                                                                                ggCAGGGGGGACTGGAGGTTGATCATGCCGTCAGGCACTTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCGCTGTCACCAggg  <  1:402762/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGAACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCGCTGTCACCAGGG  >  NC_000913/1056972‑1057238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑