Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,215,983	A→G	E65E (GAA→GAG)	ycgZ →	putative two‑component system connector protein YcgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,215,983	0	A	G	100.0%	300.2 / NA	86	E65E (GAA→GAG)	ycgZ	putative two‑component system connector protein YcgZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/55);  total (31/55)

AAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAACAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGAAATTACTGGCCAGGCGGT  >  NC_000913/1215847‑1216120                                                                                                                                         |                                                                                                                                          aaGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        <  1:202889/140‑1 (MQ=255) aaGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        <  1:104818/140‑1 (MQ=255) aaGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        <  1:791379/140‑1 (MQ=255)  aGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGa                                                                                                                                       <  2:482257/140‑1 (MQ=255)  aGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGa                                                                                                                                       <  1:180464/140‑1 (MQ=255)  aGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGa                                                                                                                                       <  2:2913/140‑1 (MQ=255)  aGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGa                                                                                                                                       <  1:747836/140‑1 (MQ=255)   gCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGaa                                                                                                                                      <  1:860127/140‑1 (MQ=255)    cTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGaaa                                                                                                                                     <  2:474006/140‑1 (MQ=255)      aaCTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACa                                                                                                                                   <  1:118808/140‑1 (MQ=255)          tGCATACTCAGCAGGCAACTCTAGGCGAACTTGTGACCGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATtat                                                                                                                               <  1:234393/140‑1 (MQ=255)          tGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATtat                                                                                                                               <  1:637372/140‑1 (MQ=255)            cATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATaa                                                                                                                             <  1:865961/140‑1 (MQ=255)             aTACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        >  1:814090/1‑128 (MQ=255)             aTACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        <  2:814090/128‑1 (MQ=255)             aTACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAAt                                                                                                                            >  2:798684/1‑140 (MQ=255)             aTACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAAt                                                                                                                            >  2:687292/1‑140 (MQ=255)              tACTCAGCAGGAAACTCTCGGGGAACTTGAGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATg                                                                                                                           <  1:380596/140‑1 (MQ=255)              tACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCTACTGGGGAAGAGGAGCAGAAACATTATAATg                                                                                                                           <  2:63456/140‑1 (MQ=255)              tACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATg                                                                                                                           <  1:852835/140‑1 (MQ=255)                cTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCa                                                                                                                         <  2:83000/140‑1 (MQ=255)                cTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCa                                                                                                                         <  2:823147/140‑1 (MQ=255)                  cagcagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        <  1:41101/123‑1 (MQ=255)                  cagcagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAg                                                                                                                                        >  2:41101/1‑123 (MQ=255)                   agcagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTa                                                                                                                      >  2:520598/1‑140 (MQ=255)                     cagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAt                                                                                                                    >  1:29584/1‑140 (MQ=255)                     cagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAt                                                                                                                    >  1:458200/1‑140 (MQ=255)                     cagGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAt                                                                                                                    >  1:493465/1‑140 (MQ=255)                      agGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAtt                                                                                                                   >  2:72399/1‑140 (MQ=255)                      agGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAtt                                                                                                                   >  1:716020/1‑140 (MQ=255)                      agGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAAtt                                                                                                                   >  1:170340/1‑140 (MQ=255)                      agGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTCGAACACGCAACTAGGGTAGAGGAGCCGAAACATTATAATGCACTAAtt                                                                                                                   >  2:324575/1‑140 (MQ=255)                        gAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGCAACATTGTAATGCACTAATTgg                                                                                                                 >  1:296519/1‑140 (MQ=255)                           aCTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGtt                                                                                                              <  2:458200/140‑1 (MQ=255)                           aCTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGtt                                                                                                              <  1:754264/140‑1 (MQ=255)                           aCTCTCGGGGAAATTATGACTGCGACTTTGAAAGATGTACGTAATCTAAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGtt                                                                                                              <  1:365666/140‑1 (MQ=255)                               tcGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCAACTGGGGAGGAGGAGCAGAAACATTGTAATCCACTAATTGGGTTGCtt                                                                                                          >  2:365239/1‑140 (MQ=255)                                  gggAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCttttt                                                                                                       <  1:520598/140‑1 (MQ=255)                                     aaaTTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGaa                                                                                                    <  1:798684/140‑1 (MQ=255)                                       aTTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTAGAATACACTAATTGGGTTGCTTTTTGaata                                                                                                  >  1:379578/1‑140 (MQ=255)                                             aCTGAGATATTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCaa                                                                                            >  2:141006/1‑140 (MQ=255)                                               tGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTAAAATGCACTAATTGGATTGCTTATTGAATAATGCAACa                                                                                          >  1:247144/1‑140 (MQ=255)                                                 agaTTTTGAAAGATGGGGGTAAACTGAGTCGCAAGACGCTTTGTGCCAGACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGc                                                                                        <  2:379578/140‑1 (MQ=255)                                                  gaTTTTGAAAGATGGTCGTAATGTGCGTCGAAAGTCGTTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGcc                                                                                       <  1:365239/140‑1 (MQ=255)                                                  gaTTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGcc                                                                                       >  2:449763/1‑140 (MQ=255)                                                   aTTTTGAAAGATGGACGTAATCCGATTCGTAAGTCGCCTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCt                                                                                      <  1:252134/140‑1 (MQ=255)                                                   aTTTTGAAAGATGGACGCAATCTGAGTCGAACGTCCCTTCGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCt                                                                                      <  2:247144/140‑1 (MQ=255)                                                    ttttGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGAACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTg                                                                                     <  2:201024/140‑1 (MQ=255)                                                     tttGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGa                                                                                    >  2:722518/1‑140 (MQ=255)                                                      ttGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGat                                                                                   <  1:722518/140‑1 (MQ=255)                                                             aTGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCaa                                                                                            <  2:699915/124‑1 (MQ=255)                                                             aTGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTaa                                                                            <  1:141006/140‑1 (MQ=255)                                                             aTGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTaa                                                                            >  1:762339/1‑140 (MQ=255)                                                             aTGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCCTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCaa                                                                                            >  1:699915/1‑124 (MQ=255)                                                              tGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAATTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAAc                                                                           <  1:5410/140‑1 (MQ=255)                                                                 aCGTAATCTGAGTCGAAGGTCGCTTTGTGCCCAACATCTCTGCCGACTCGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAgg                                                                        <  2:252552/140‑1 (MQ=255)                                                                  cGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGt                                                                       <  1:687292/140‑1 (MQ=255)                                                                  cGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGt                                                                       <  1:449763/140‑1 (MQ=255)                                                                  cGTAATCTGAGTCGAAAGTCGCGTTGTGCCATACTTCTTAGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGt                                                                       <  2:296519/140‑1 (MQ=255)                                                                   gTAATCTGAGTCGAATGTCGCTTTGGGCCTAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGtt                                                                      <  1:282107/140‑1 (MQ=255)                                                                   gTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGtt                                                                      <  2:834879/140‑1 (MQ=255)                                                                      aTCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGc                                                                   <  1:208377/140‑1 (MQ=255)                                                                      aTCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGc                                                                   >  1:840143/1‑140 (MQ=255)                                                                          gAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCATCAGGCTGATTATTGAACAGGTTAGCTATg                                                               >  1:252552/1‑140 (MQ=255)                                                                           aGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGGACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGa                                                              >  2:840074/1‑140 (MQ=255)                                                                           aGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGa                                                              >  1:49373/1‑140 (MQ=255)                                                                                aaaGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCg                                                         <  2:29584/140‑1 (MQ=255)                                                                                  aGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGtt                                                       <  1:86644/140‑1 (MQ=255)                                                                                  aGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGtt                                                       <  1:551652/140‑1 (MQ=255)                                                                                       ttttGTGCCAAACTTCTGTGCCGACTGGAACACGCCACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGaa                                                  <  2:493465/139‑1 (MQ=255)                                                                                       gtttGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCTCTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGaa                                                  <  2:716020/139‑1 (MQ=255)                                                                                        tttGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAg                                                 <  1:840074/140‑1 (MQ=255)                                                                                          tgtgCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGAc                                               <  1:72399/140‑1 (MQ=255)                                                                                          tgtgCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGAc                                               <  2:506326/140‑1 (MQ=255)                                                                                          tgtgCCAAACTTCTTTGCCGACGGGAACAAGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGAc                                               <  1:324575/140‑1 (MQ=255)                                                                                           gtgCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACa                                              >  2:643465/1‑140 (MQ=255)                                                                                            acagaaaCTTCTTTGTCGACTGTAAGACGCGACTGGGAAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACAt                                             <  1:387441/136‑1 (MQ=255)                                                                                             gCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATc                                            <  1:793227/140‑1 (MQ=255)                                                                                                           ccGACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTAAACAGGTTAGCTATGAAGTCGTTATGAAGAAGTCTGATAATGAActgt                              >  2:387441/1‑137 (MQ=255)                                                                                                             gACTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTACCAGGTTAGCTATCAAGCCGTTATGAAGACAGCTGATAATGAACGtata                            >  2:320557/1‑140 (MQ=255)                                                                                                              aCTGGAACACGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATaa                           >  1:509904/1‑140 (MQ=255)                                                                                                                     cacGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGa                    <  2:509904/140‑1 (MQ=255)                                                                                                                     cacGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGa                    >  2:767106/1‑140 (MQ=255)                                                                                                                     cacGCGACTGGGGAAGAGGAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGa                    <  1:643465/140‑1 (MQ=255)                                                                                                                                     agGAGCAGAAACATTATAATGCACTAATTGGGTTGGTTTCTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGAAATTACTGGCCAGGCg    <  2:365946/140‑1 (MQ=255)                                                                                                                                      ggAGCAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGAAATTACTGGCCAGGCgg   >  1:55198/1‑140 (MQ=255)                                                                                                                                       agaCAGAAACATTATATTGCACGAATTGGGTTGTTTTTTCAATAATGCTACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGAAATTACTGGCCAGGCGGt  <  2:382856/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          AAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGATGGACGTAATCTGAGTCGAAAGTCGCTTTGTGCCAAACTTCTTTGCCGACTGGAACACGCGACTGGGGAAGAGGAACAGAAACATTATAATGCACTAATTGGGTTGCTTTTTGAATAATGCAACAGCCTGATTATTTAACAGGTTAGCTATGAAGTCGTTATGAAGACATCTGATAATGAACGTATAAAATATGAAATTACTGGCCAGGCGGT  >  NC_000913/1215847‑1216120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑