Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,222,729	A→G	V87A (GTT→GCT)	ymgG ←	PF13488 family protein YmgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,729	0	A	G	100.0%	170.8 / NA	50	V87A (GTT→GCT)	ymgG	PF13488 family protein YmgG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTACGCCATTAACACCATT  >  NC_000913/1222595‑1222862                                                                                                                                       |                                                                                                                                      cGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCa                                                                                                                                  >  1:526687/1‑140 (MQ=255) aaaaaaCAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCa                                                                                                                                  <  2:775281/138‑1 (MQ=255)                 cGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAAcgg                                                                                                                  <  2:113067/140‑1 (MQ=255)                        aaaGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCAcc                                                                                                           <  2:103063/140‑1 (MQ=255)                          aGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCa                                                                                                         <  2:508903/140‑1 (MQ=255)                             aaaTTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGACATTGCCGGTCAGAGCGCCAGTGACGGCACGAACGGCGGCACCCACtt                                                                                                      >  2:256909/1‑140 (MQ=255)                              aaTTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGGCAGCACCAACGGCCGCACCCACttt                                                                                                     >  2:99055/1‑140 (MQ=255)                              aaTTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAACACCAACGGCGGCACCCACttt                                                                                                     >  2:322446/1‑140 (MQ=255)                                    ttCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCAACAACGGCGGCACCCACTTTTGCAcc                                                                                               >  1:326727/1‑140 (MQ=255)                                                   tATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCAttttt                                                                                >  2:164307/1‑140 (MQ=255)                                                   tATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCAttttt                                                                                >  2:97516/1‑140 (MQ=255)                                                      ttCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTAcc                                                                             >  1:856298/1‑140 (MQ=255)                                                          ttttACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTcg                                                                         <  1:492220/140‑1 (MQ=255)                                                          ttttACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTcg                                                                         <  1:164307/140‑1 (MQ=255)                                                             tACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACTGTCGCgg                                                                      >  1:109271/1‑140 (MQ=255)                                                             tACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCgg                                                                      <  2:526687/140‑1 (MQ=255)                                                               cccAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGcc                                                                    >  2:178795/1‑140 (MQ=255)                                                               cccAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGcc                                                                    >  2:988/1‑140 (MQ=255)                                                                ccAGAATTGCACCACCCGTACCGCCAATCACGGCACCCTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCt                                                                   <  1:322446/140‑1 (MQ=255)                                                                caggAATTGCACCACCCGTACCTCCAATCCCGGCACCCTTAATAGCCCCTTCGAGGCAATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCt                                                                   <  1:377254/137‑1 (MQ=255)                                                                       tGCACCGCCCGTACCGCCAATCACGTCACTTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTGCGCGCATTTTTACCGTCGCGGCCTTTTTCTg                                                            <  1:362893/140‑1 (MQ=255)                                                                           ccaccCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTAc                                                        <  1:178795/140‑1 (MQ=255)                                                                            caccCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTGTTGCCCCTTTACCCGCATTTTTACCGTCGCGGCCTTTTTCTGTTGCt                                                       <  2:818438/140‑1 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGc                                                     >  1:675260/1‑140 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGc                                                     >  1:780853/1‑140 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGc                                                     >  1:156247/1‑140 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGc                                                     >  2:457359/1‑140 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTATTACCGGCGCGGCATTTTTCTGTTACTGc                                                     >  1:373083/1‑140 (MQ=255)                                                                              cccGTACCGCCAATCACGGCACCTTTAATAGCCCCTTCGAGGCAATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGGGCATTTTTACCGGCGCGCCCTTTTTCTGTTAcctg                                                     >  2:377254/1‑137 (MQ=255)                                                                                 gTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACTGTCGCGGCCTTTTTCTGTTACTGCAcc                                                  <  2:109271/140‑1 (MQ=255)                                                                                  tACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCt                                                                   <  2:504435/122‑1 (MQ=255)                                                                                  tACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCt                                                                   >  1:504435/1‑122 (MQ=255)                                                                                             aCGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGcc                                      <  1:733944/140‑1 (MQ=255)                                                                                             aCGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGcc                                      <  2:780853/140‑1 (MQ=255)                                                                                                     tttAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTg                              <  1:988/140‑1 (MQ=255)                                                                                                      ttAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGc                             >  1:10562/1‑140 (MQ=255)                                                                                                      ttAATCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACAGTCGCGGCATTTATCTTTTACTGCACCAACACCAGCGCCAACAGCTGc                             >  2:299997/1‑140 (MQ=255)                                                                                                       tAATCGCCCCTTCGAGATCATTACCGGTCAGTGGGTCAGTGACAGCACCACCGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACTAGCGCCAACAGCTGCa                            <  2:373083/140‑1 (MQ=255)                                                                                                         aTCGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTACTGTTACAGCAGCACCACCAGTGCCAACAGCTGCAcc                          >  1:270977/1‑140 (MQ=255)                                                                                                          tCGCCCCTTCGAGGCCAGTGCCGGTCAGAGCGCCATTGATAGCACCAATGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCt                         <  2:326727/140‑1 (MQ=255)                                                                                                           cGCCCCTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCtt                        >  2:722170/1‑140 (MQ=255)                                                                                                             ccccTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTc                      >  2:683705/1‑140 (MQ=255)                                                                                                              cccTTCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCa                     >  1:442001/1‑140 (MQ=255)                                                                                                                 ttCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTa                  >  1:656969/1‑140 (MQ=255)                                                                                                                  tCGAGGCCATTGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTAc                 <  1:683705/140‑1 (MQ=255)                                                                                                                           ttGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTAATGCACCAAAACCTGCGCCAACAGCTTCACCCTTCAGTACGCCATTAac        >  2:368340/1‑140 (MQ=255)                                                                                                                            tGCCGGTCAGAGCGCTAGTGACTGCGCCACCGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTACGCCATTAaca       <  1:368340/140‑1 (MQ=255)                                                                                                                            tGCCGGTCAGAGCGCCATTGACCGCACCAACTGCGCCACACACTTTTGTACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTACGCCATTAaca       <  2:240849/140‑1 (MQ=255)                                                                                                                            tGCCGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACGGCACCAACGCCAACGCAGACAGCTGCACCTAGCAGTACGCCATTAaca       >  2:418540/1‑140 (MQ=255)                                                                                                                               cGGTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTACGCCATTAACACCa    <  1:722170/140‑1 (MQ=255)                                                                                                                                 gTCAGAGCGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGTCAACAGCGGCAGCTGTCAGTAGGCCATTAACACCAtt  >  1:327607/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACCAACGGCGGCACCCACTTTTGCACCTTTACGCGCATTTTTACCGTCGCGGCCTTTTTCTGTTACTGCACCAACACCAGCGCCAACAGCTGCACCTTTCAGTACGCCATTAACACCATT  >  NC_000913/1222595‑1222862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑