Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,266,239	A→G	D49G (GAC→GGC)	prmC →	protein‑(glutamine‑N(5)) methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,266,239	0	A	G	100.0%	118.9 / NA	36	D49G (GAC→GGC)	prmC	protein‑(glutamine‑N(5)) methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

CAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGA  >  NC_000913/1266103‑1266374                                                                                                                                         |                                                                                                                                        cAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGa                                                                                                                                      <  2:747/140‑1 (MQ=255)   acacTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAAc                                                                                                                                    >  1:100412/1‑140 (MQ=255)    cacTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACa                                                                                                                                   >  2:637486/1‑140 (MQ=255)         gTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTGGCCTTTGCTGATACACAGCTGGCTGGCGAACAATGTc                                                                                                                              >  1:232867/1‑140 (MQ=255)         gTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTc                                                                                                                              >  1:681300/1‑140 (MQ=255)          ttACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCa                                                                                                                             >  1:568955/1‑140 (MQ=255)                gAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACt                                                                                                                       <  1:663705/140‑1 (MQ=255)                    cAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGAt                                                                                                                   <  2:568955/140‑1 (MQ=255)                     aaTAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATg                                                                                                                  <  1:778803/140‑1 (MQ=255)                       tAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATgcg                                                                                                                >  2:548847/1‑140 (MQ=255)                            cAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGGTGACTGGTGAAGAAAGTCAGCGACTTGATGCGCTACt                                                                                                           >  1:250409/1‑140 (MQ=255)                            cAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACt                                                                                                           >  1:213903/1‑140 (MQ=255)                              aCTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGa                                                                                                         >  2:762273/1‑140 (MQ=255)                              aCTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGa                                                                                                         >  2:577379/1‑140 (MQ=255)                                ttCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGACACGCAGCTAACTGGCGTGCAATGTCTGCAAAGTGATGCGCTACTGaca                                                                                                       >  2:412680/1‑140 (MQ=255)                                ttCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGaca                                                                                                       <  1:577379/140‑1 (MQ=255)                                        agcgaAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGtcgtcg                                                                                               <  1:762273/140‑1 (MQ=255)                                        agcgaAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGtcgtcg                                                                                               <  1:637486/140‑1 (MQ=255)                                          cgaAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTcgcg                                                                                             >  2:582877/1‑140 (MQ=255)                                          cgaAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTcgcg                                                                                             >  1:616485/1‑140 (MQ=255)                                          cgaAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTcgcg                                                                                             <  2:681300/140‑1 (MQ=255)                                                cccGCGGCGTGATGCTGTAATCCTGCTGGAGCATGATACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTg                                                                                       <  2:224833/140‑1 (MQ=255)                                                 ccGCGGCGTGATGCTGACATCCAGCTGGAGCCTGTCACCGGCAGAGGGAGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGa                                                                                      <  2:250409/140‑1 (MQ=255)                                                 ccGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGa                                                                                      <  2:213903/140‑1 (MQ=255)                                                    cggcgTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCTCTACTGCCACGTCATCGCGATGGTGAAcc                                                                                   >  1:301965/1‑140 (MQ=255)                                                           aTGCTGAACTCCTGCTGAAGCAAGTTACCGGAATACGGCATACTTTTAATCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCt                                                                            <  2:419378/140‑1 (MQ=255)                                                           aTGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCt                                                                            <  1:19904/140‑1 (MQ=255)                                                                           ggAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAACGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCgggg                                                               >  1:141474/1‑137 (MQ=255)                                                                           ggAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAACGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCgggg                                                               <  2:141474/137‑1 (MQ=255)                                                                             aGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCga                                                          <  1:548847/140‑1 (MQ=255)                                                                                    tACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCt                                                   >  2:7710/1‑140 (MQ=255)                                                                                         gCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCg                                              <  2:5203/140‑1 (MQ=255)                                                                                                         tATTCTCGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGttt                              <  1:582877/140‑1 (MQ=255)                                                                                                              tcGCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCAGTTATTTGTTTCGCCa                         <  1:727497/140‑1 (MQ=255)                                                                                                                gCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGc                       >  1:623543/1‑140 (MQ=255)                                                                                                                gCCTTTGGTGAAACGCAGCTGACTGGCGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGc                       >  1:464480/1‑140 (MQ=255)                                                                                                                                     actggcGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGa  >  1:5270/1‑140 (MQ=255)                                                                                                                                     actggcGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTATTTCGCCAACGACCTTAATTCCGCGCCCGGa  >  2:329197/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACACGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGA  >  NC_000913/1266103‑1266374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑