Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,183	A→G	C647R (TGT→CGT)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,183	0	A	G	100.0%	99.1 / NA	29	C647R (TGT→CGT)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

TTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAAC  >  NC_000913/1296046‑1296317                                                                                                                                          |                                                                                                                                       ttCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGcacgca                                                                                                                                      <  2:684126/140‑1 (MQ=255)          ggACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTAACTGCGTTCAGACCTCCGAAAGCACGCAGGGACTTCg                                                                                                                             >  1:315920/1‑140 (MQ=255)            aCGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGc                                                                                                                           <  2:837112/140‑1 (MQ=255)            aCGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGc                                                                                                                           <  2:432516/140‑1 (MQ=255)                            tttCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGt                                                                                                           >  1:772693/1‑140 (MQ=255)                                     tttCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGa                                                                                                  >  1:614424/1‑140 (MQ=255)                                       tCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACa                                                                                                <  2:752348/140‑1 (MQ=255)                                            gCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGc                                                                                           >  2:57629/1‑140 (MQ=255)                                                gCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCata                                                                                       <  2:560778/140‑1 (MQ=255)                                                        tGACCATCAGAGAACTCTGATGACAGTACAGTAACATAAGCTTCCATGACGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGt                                                                               <  2:315920/140‑1 (MQ=255)                                                        tGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGt                                                                               <  1:150871/140‑1 (MQ=255)                                                                    aaCTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGACATGTCCATAACCAGGTTGGCGTCGACGATCGCCATATCCGGAGTCAGCGCATAGTc                                                                   >  2:547979/1‑140 (MQ=255)                                                                     aCTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATTACCAGGTTAGCGACGATAATCGCCATATCCGGAGTCAGCGCATAGTCt                                                                  >  2:308293/1‑140 (MQ=255)                                                                         aGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCa                                                              <  2:772693/140‑1 (MQ=255)                                                                              ccAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGa                                                         <  2:614424/140‑1 (MQ=255)                                                                                aGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGata                                                       >  1:456345/1‑140 (MQ=255)                                                                                   aCAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATAttt                                                    <  1:730827/140‑1 (MQ=255)                                                                                   aCAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATAttt                                                    <  2:456345/140‑1 (MQ=255)                                                                                                 ttCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAATAGcgc                                      >  2:208065/1‑139 (MQ=255)                                                                                                   ccATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGcgtcg                                    >  1:84767/1‑140 (MQ=255)                                                                                                     aTGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCACAGTCTGCGAGCGGACATTTCTAACCAGTAGCgtcgtc                                  >  1:245751/1‑140 (MQ=255)                                                                                                             aGTTACTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTAAGTTACaa                          >  2:92731/1‑140 (MQ=255)                                                                                                                  cTGCGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCa                     >  1:765494/1‑140 (MQ=255)                                                                                                                     cGTCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTAAGTTACAACCGCAAAc                  <  1:92731/140‑1 (MQ=255)                                                                                                                       tCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACgg                >  2:446095/1‑140 (MQ=255)                                                                                                                       tCCAGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACgg                >  1:37544/1‑140 (MQ=255)                                                                                                                          aGACCACCGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGt             <  2:27678/140‑1 (MQ=255)                                                                                                                            accaccGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTg            <  2:705167/139‑1 (MQ=255)                                                                                                                            accaccGAAAGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTg            >  1:705167/1‑139 (MQ=255)                                                                                                                                  gAAAGCACGCAGGGATTTCGGCTTGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAg     <  1:308293/140‑1 (MQ=255)                                                                                                                                     aGCACGCAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAAc  <  2:765494/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTGGCGTCGACAATCGCCATATCCGGAGTCAGCGCATAGTCTGCCAGCGGATATTTCTGACCAGTAGCGTCGTCAGTTACAACCGCAAACGGAGTGACTTCAGAAC  >  NC_000913/1296046‑1296317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑