Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,326,396	A→G	T551A (ACA→GCA)	yciQ →	DUF2207 domain‑containing protein YciQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,326,396	0	A	G	100.0%	108.2 / NA	31	T551A (ACA→GCA)	yciQ	DUF2207 domain‑containing protein YciQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

ACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATT  >  NC_000913/1326261‑1326529                                                                                                                                        |                                                                                                                                      aCTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACg                                                                                                                                   >  2:704261/1‑140 (MQ=255)   tGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGa                                                                                                                                 >  2:121137/1‑140 (MQ=255)               tatTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTAcg                                                                                                                     >  2:761328/1‑140 (MQ=255)               tatTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTAcg                                                                                                                     <  2:698214/140‑1 (MQ=255)               tatTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTAcg                                                                                                                     >  2:694407/1‑140 (MQ=255)               tatTTTTCTGCCAAAGTCTACCTAAACTGGATAGCCACGCTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTAcg                                                                                                                     <  2:240469/140‑1 (MQ=255)               tatTTTTCTGCCAAACTTTACCGAATCTGCACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTAcg                                                                                                                     <  1:408673/140‑1 (MQ=255)                    ttCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCAGGGAAACTGGATTACGCGTTg                                                                                                                >  1:160624/1‑140 (MQ=255)                    ttCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTg                                                                                                                <  2:627216/140‑1 (MQ=255)                    ttCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTg                                                                                                                <  2:216360/140‑1 (MQ=255)                     tCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGc                                                                                                               >  2:654332/1‑140 (MQ=255)                       tGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTa                                                                                                             >  1:449197/1‑140 (MQ=255)                                                    cGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGgggag                                                                                <  1:694407/140‑1 (MQ=255)                                                                 gTGAAGCTGTCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTa                                                                   <  2:193439/140‑1 (MQ=255)                                                                           cGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGcct                                                         >  2:167881/1‑139 (MQ=255)                                                                                      ttGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTttct                                              >  2:77766/1‑140 (MQ=255)                                                                                       tGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTttctt                                             >  1:184438/1‑140 (MQ=255)                                                                                         cgcgTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCg                                           >  2:631399/1‑140 (MQ=255)                                                                                              aaaGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGAt                                      >  2:39995/1‑140 (MQ=255)                                                                                                 gagGCAGCAACACACTGTGGACGTCAGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTcg                                   <  1:368430/140‑1 (MQ=255)                                                                                                 gagGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTcg                                   <  2:578311/140‑1 (MQ=255)                                                                                                  agGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGTGCGCTACTCATTGCCCGTTCGATTTCTTCGGCGATTcgc                                  >  2:368430/1‑140 (MQ=255)                                                                                                      agcaACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTc                              <  1:77766/140‑1 (MQ=255)                                                                                                      agcaACACACAGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTc                              <  2:27003/140‑1 (MQ=255)                                                                                                               caGTGGACGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCTCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTg                     <  1:167881/140‑1 (MQ=255)                                                                                                                     aCGTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTa               >  1:209186/1‑140 (MQ=255)                                                                                                                       gTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAAc             >  2:27203/1‑140 (MQ=255)                                                                                                                       gTCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGAGTCGCGCTCCTGAGATTGCCCGTAAc             >  1:368552/1‑140 (MQ=255)                                                                                                                        tCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACg            <  1:704261/140‑1 (MQ=255)                                                                                                                        tCGGCGGAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACg            <  1:761328/140‑1 (MQ=255)                                                                                                                          ggcggAAAGGGGAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGc          >  2:158497/1‑140 (MQ=255)                                                                                                                                  ggggAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTAtt  <  1:39995/140‑1 (MQ=255)                                                                                                                                  ggggAAGCACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTAtt  <  1:27203/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATT  >  NC_000913/1326261‑1326529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑