Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,451	A→G	T207T (ACA→ACG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,451	0	A	G	100.0%	152.2 / NA	43	T207T (ACA→ACG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

GGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGT  >  NC_000913/1336315‑1336579                                                                                                                                         |                                                                                                                                 ggggAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCGTATCCGGATACGCTc                                                                                                                               >  1:356403/1‑140 (MQ=255)   ggAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGt                                                                                                                             >  1:104114/1‑140 (MQ=255)   ggAAAGCAAGCGTTCAGTCGGTGTAGCGTCGAGCCGCCATGCACAGGCCTTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGt                                                                                                                             <  2:356403/140‑1 (MQ=255)    gAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGtt                                                                                                                            >  1:541033/1‑140 (MQ=255)            gcGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATGGCAGGACGGTGAATGGGTTGCTTATGCGGATACGCTCGTTGGTACTGa                                                                                                                    >  2:298192/1‑140 (MQ=255)             cGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGAc                                                                                                                   <  1:693289/140‑1 (MQ=255)             cGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTCGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGAc                                                                                                                   <  1:51761/140‑1 (MQ=255)                tCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCg                                                                                                                <  1:863543/140‑1 (MQ=255)                tCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCg                                                                                                                <  2:541033/140‑1 (MQ=255)                tCAGTCGGTTTAGCGTCGTGCCACCAGGCACTGGCATTTTCCATCAGGATAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCg                                                                                                                <  2:274493/140‑1 (MQ=255)                    tCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGcaca                                                                                                            <  1:126429/140‑1 (MQ=255)                    tCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGcaca                                                                                                            <  1:805399/140‑1 (MQ=255)                                 gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCaa                                                                                               >  2:782353/1‑140 (MQ=255)                                  tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGcaca                                                                                                            >  2:813833/1‑126 (MQ=255)                                  tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGcaca                                                                                                            <  1:813833/126‑1 (MQ=255)                                  tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCGTATCCGGAGACGGTCGGTGGTACTGTCTCGCACACCACCATGATCAAc                                                                                              >  2:269173/1‑140 (MQ=255)                                              caggcaTTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTg                                                                                  <  2:510195/140‑1 (MQ=255)                                                gagacaggCCATCAGGTTAACCACGAATATCTCGGTAAAGCAGTGTGGGGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCt                                                                                <  2:349350/133‑1 (MQ=255)                                                    tttGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCtgggg                                                                            >  2:810007/1‑140 (MQ=255)                                                     ttGCCATCAGGTTAACCTCGAAAATCTCGCCAAAGCACTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCtggggt                                                                           <  1:298192/140‑1 (MQ=255)                                                            cAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGt                                                                    >  2:140344/1‑140 (MQ=255)                                                                ttAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTtggt                                                                <  1:855613/140‑1 (MQ=255)                                                                  aaCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTTGCTTGCTGGGGTGGGGCGCtgtctc                                                              >  1:349350/1‑136 (MQ=255)                                                                         aaTATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGaa                                                       >  1:471900/1‑140 (MQ=255)                                                                             tctcGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTGGGCGTGCAGGGTTGGGGCGTTGGTGGGATCGAAGCAg                                                   >  1:284155/1‑140 (MQ=255)                                                                                   agaaGCAGTGTGGAGTGAATTGAAGCACGGTGAATGGCTTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCg                                             <  1:353553/138‑1 (MQ=255)                                                                                           tgtgGAGTGAATTGTAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTa                                     >  1:797939/1‑140 (MQ=255)                                                                                             tgGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAgg                                   >  1:824243/1‑140 (MQ=255)                                                                                              ggAGTGAATTGCAGGACCGTGAATGGATTGCCTATCCGGATACGCTCGATGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGc                                  <  2:268276/140‑1 (MQ=255)                                                                                               gAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGcc                                 <  1:140344/140‑1 (MQ=255)                                                                                                aGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCCGGGGTGGGCCGTCGGTGGGATCGAAACAGAAGCCGCAATGTTAGGcct                                >  2:353553/1‑139 (MQ=255)                                                                                                  tGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTAGGTGGGAACGAGGCAGAAGCCGCAATGTTAGGCCAGc                              >  1:268276/1‑140 (MQ=255)                                                                                                    aaTTGTAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCg                            <  2:797939/140‑1 (MQ=255)                                                                                                     aTTGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCgg                           >  2:654894/1‑140 (MQ=255)                                                                                                       tGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGtt                         >  2:205729/1‑140 (MQ=255)                                                                                                       tGCAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGtt                         >  1:683648/1‑140 (MQ=255)                                                                                                         cAGGACGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTc                       >  2:109289/1‑140 (MQ=255)                                                                                                              cGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGc                  <  2:824243/140‑1 (MQ=255)                                                                                                              cGGTGAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGc                  <  1:654894/140‑1 (MQ=255)                                                                                                                  gAATGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTAt              <  1:205729/140‑1 (MQ=255)                                                                                                                   aaTGGATTGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGTTCAACGGCCTTGGCGTGCTGGGGGGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCATTGTTAGGCCAGCCCGCTTCCATGCTTATc             >  1:313933/1‑140 (MQ=255)                                                                                                                          tGCTTATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATg      >  2:204279/1‑140 (MQ=255)                                                                                                                              tATCCGGATACGCTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGt  <  2:104114/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGT  >  NC_000913/1336315‑1336579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑