| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,357,671 | T→G | intergenic (‑3/+131) | ymjA ← / ← puuP | DUF2543 domain‑containing protein YmjA/putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,357,671 | 0 | T | G | 96.9% | 104.6 / NA | 32 | intergenic (‑3/+131) | ymjA/puuP | DUF2543 domain‑containing protein YmjA/putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (14/17); minor base A (1/0); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTA > NC_000913/1357535‑1357801 | ttCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTg > 2:98598/1‑140 (MQ=255)ttCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTg > 1:593979/1‑140 (MQ=255) gggTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGGGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGt < 2:107106/140‑1 (MQ=255) gggTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGt < 2:614800/140‑1 (MQ=255) gAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTGATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAAt < 2:720989/140‑1 (MQ=255) gTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCt < 2:509059/140‑1 (MQ=255) tAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTa < 2:191164/140‑1 (MQ=255) aCTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATTGTGGTTCATTAATTGCCCCTGTGGTTAATGCGCTAAg > 1:392510/1‑140 (MQ=255) aCTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGcccc < 1:566156/122‑1 (MQ=255) aCTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGcccc > 2:566156/1‑122 (MQ=255) aaGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATaa < 1:98598/140‑1 (MQ=255) tagtGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCGTTAGTTGACCCGGGGGTTAATGCGCTAAGCATAAggg > 1:226946/1‑140 (MQ=255) tagtGGGCCAGCGGTGTGCGTTAAGCCTCTGTGACGGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAggg < 2:392510/140‑1 (MQ=255) gCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGgatg > 2:7696/1‑140 (MQ=255) gCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTAGTTCATTAATTGACACTGGGGTTAAAGCGCTAAGCATAACGGCGgatg > 2:404315/1‑140 (MQ=255) ccAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTCATGCGCTAAACATAAGGGCGgatga > 1:309489/1‑140 (MQ=255) gCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAg > 1:781082/1‑140 (MQ=255) gCGGTGTGCGTTCAGCCCCTGCCACAGGCTCGGCGCATTTGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAg < 2:409196/140‑1 (MQ=255) gCGGTGTGCGTTCAGCCACTGCGACAGGCTCTGCGCATTGGGTCGCGTGCTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAg < 2:309489/140‑1 (MQ=255) tgtgCGTTCAGCCTCCGCGACACGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACt < 1:251501/140‑1 (MQ=255) tgCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCg < 1:739578/126‑1 (MQ=255) tgCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCg > 2:739578/1‑126 (MQ=255) tgCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCg > 1:782690/1‑140 (MQ=255) tgCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCg > 1:511516/1‑140 (MQ=255) ttCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGAt < 1:595632/140‑1 (MQ=255) tCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAACCATTAGCGCGGATTATAGCACTCGCGATc > 1:352059/1‑140 (MQ=255) ctgCCTCTGAGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTATAGCGGGCTATCGTGGTACATCAGTTGCCCCTAGGTTTAATGCGCTAAGCATAAGGGCGGATGATGGCTCTCGTGATcc < 2:860158/138‑1 (MQ=255) aGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCg < 2:593979/140‑1 (MQ=255) ctGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCGAAGAATACGGGCGGATGATAGGACTCGTGATCCGGGTCa > 2:235450/1‑140 (MQ=255) gCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCttt > 2:808789/1‑140 (MQ=255) tCGCGTACTCATCGGAAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGACGATAGCACTCGTGGTCCGGGTCATACCTCTTTTTATAGTTAAAggcg < 2:147124/140‑1 (MQ=255) tatCAAAATATTTTAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTcagg < 1:808789/140‑1 (MQ=255) tCAAAATATTTTAGCGGGATATAGTGGTTCAATAGGTGCGCCTGGGGTTAATTCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTcaggcg < 2:352059/140‑2 (MQ=255) tttAGCGGGATATCGTGGTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATgc > 2:858024/1‑140 (MQ=255) cGTGGTTCATTAGTTACCCCTGAGGATAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGa < 1:404315/140‑1 (MQ=255) ggTTCATTAGTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTa < 2:511516/140‑1 (MQ=255) | TTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATACATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTA > NC_000913/1357535‑1357801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |