Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,423,575 A→G pseudogene (231/300 nt)
N59S (AAC→AGC) 		 rzpR →
rzoR →		 Rac prophage; putative endopeptidase RzpR
Rac prophage; putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,423,5750AG100.0% 205.2 / NA 59pseudogene (231/300 nt)
N59S (AAC→AGC) 		rzpR
rzoR		Rac prophage; putative endopeptidase RzpR
Rac prophage; putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

GCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTAAGATCTAA  >  NC_000913/1423441‑1423709
                                                                                                                                      |                                                                                                                                      
gCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACt                                                                                                                                   >  2:566545/1‑140 (MQ=255)
   ggTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGt                                                                                                                                >  2:637007/1‑140 (MQ=255)
              gTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAgg                                                                                                                     >  1:817817/1‑140 (MQ=255)
              gTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAgg                                                                                                                     >  2:601246/1‑140 (MQ=255)
               ttGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCTGGAGCGACTGGTAATGATGCAGGc                                                                                                                    <  1:723867/140‑1 (MQ=255)
                tGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGcc                                                                                                                   <  1:99122/140‑1 (MQ=255)
                   acaTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTCCACCCTCCGGGAGCAACTGGTGATGATGCAGGCCCaa                                                                                                                >  1:363385/1‑140 (MQ=255)
                        aaaGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGa                                                                                                           <  2:55952/140‑1 (MQ=255)
                          aGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAg                                                                                                         >  2:198442/1‑140 (MQ=255)
                          aGCAGTCTGTCAGTCAGCGCGTCAAGCCACCCCCGTCTCTGGCGTGGACAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAg                                                                                                         <  2:228146/140‑1 (MQ=18)
                               tCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGcc                                                                                                                   >  2:133469/1‑125 (MQ=255)
                               tCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGcc                                                                                                                   <  1:133469/125‑1 (MQ=255)
                               tCTGTCAGTCAGTGCATGAAGCTACTACCGCCTCCGGTGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCt                                                                                                    <  1:409947/140‑1 (MQ=18)
                                 tGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATACAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTg                                                                                                      <  2:139441/136‑1 (MQ=255)
                                 tGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATACAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTg                                                                                                      >  1:139441/1‑136 (MQ=255)
                                  gtcagccaGTGTGGGAGGCCACCCCCGCCTCCCGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCTCCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAg                                                                                                 <  2:7894/140‑1 (MQ=21)
                                   tcagtcagTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGc                                                                                                <  2:507060/140‑1 (MQ=255)
                                   tcagtcagTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGc                                                                                                >  1:50873/1‑140 (MQ=255)
                                    cagtcggtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCa                                                                                               <  2:642877/140‑1 (MQ=255)
                                      gtcagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAAt                                                                                             >  2:849863/1‑140 (MQ=255)
                                         agtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACa                                                                                          <  2:50873/140‑1 (MQ=255)
                                         agtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACa                                                                                          >  2:516830/1‑140 (MQ=255)
                                           tGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATa                                                                                        >  2:794947/1‑140 (MQ=255)
                                              gTGAAGCCTCCACCGCCACCGGCGTGGATAATGCATCCTCCCCCCGACAGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAAcc                                                                                     <  2:413462/140‑1 (MQ=25)
                                              gTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAAcc                                                                                     <  1:516830/140‑1 (MQ=255)
                                                 aaGCCACCACCGCCTCCAGCGTTGATAATGCTGCCACCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAg                                                                                  <  2:373302/140‑1 (MQ=25)
                                                  aGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGc                                                                                 <  1:176257/140‑1 (MQ=255)
                                                  aGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGc                                                                                 <  1:601246/140‑1 (MQ=255)
                                                     caccacCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAgt                                                                              >  2:751942/1‑140 (MQ=255)
                                                      accaccGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAgtg                                                                             >  2:82051/1‑140 (MQ=255)
                                                       ccaccGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAgtgt                                                                            >  2:687148/1‑140 (MQ=255)
                                                             ccTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAg                                                                      >  1:176543/1‑140 (MQ=255)
                                                                   gCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAAt                                                                <  1:751942/140‑1 (MQ=255)
                                                                      tGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCtt                                                             <  2:489708/140‑1 (MQ=255)
                                                                      gggATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCtt                                                             <  2:149737/139‑1 (MQ=255)
                                                                                  ccTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAAGCGAGCAGAGTTGAAAGTAAAAACTTAACTACAATATg                                                 >  1:277043/1‑140 (MQ=38)
                                                                                  ccTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCtt                                                             <  1:812340/128‑1 (MQ=255)
                                                                                  ccTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCtt                                                             >  2:812340/1‑128 (MQ=255)
                                                                                   cTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGa                                                >  2:61115/1‑140 (MQ=255)
                                                                                     ccccccGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGCGCAGTGTCTAAAGTAAAATCTGAACTACAATATGAtt                                              >  1:270745/1‑140 (MQ=255)
                                                                                       ccccGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCa                                            >  1:171715/1‑140 (MQ=255)
                                                                                       ccccGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCa                                            >  1:101284/1‑140 (MQ=255)
                                                                                         ccGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCAtt                                          <  1:566545/140‑1 (MQ=255)
                                                                                                gCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAGAGTAAAAGCTTGACCACAATATGATTCATTTtgatga                                   >  1:240044/1‑140 (MQ=255)
                                                                                                gCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTtgatga                                   <  2:140513/140‑1 (MQ=255)
                                                                                                gCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTtgatga                                   <  1:637007/140‑1 (MQ=255)
                                                                                                        cGCTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCa                           >  1:651082/1‑140 (MQ=255)
                                                                                                          cTGAACGGGATTATTTCACCCTTCGGGAGCGTCTGCTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATa                         <  2:240044/140‑1 (MQ=255)
                                                                                                          cTGAACGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATa                         >  2:828334/1‑140 (MQ=255)
                                                                                                             aaCGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAgg                      <  1:502109/140‑1 (MQ=255)
                                                                                                              aCGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGa                     <  1:61115/140‑1 (MQ=255)
                                                                                                               cGGGATTATTTCGCCCTTCGGGTGCGACTGGGAATGATGAAGGCCCAAGTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGaa                    <  2:363385/140‑1 (MQ=255)
                                                                                                               cGGGATTATTTCACCCTCCGGGCGCGACTGGCAATCATGAAGGCCCAAATTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGaa                    <  2:244842/140‑1 (MQ=255)
                                                                                                               cGGGATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGaa                    <  2:817817/140‑1 (MQ=255)
                                                                                                                  gATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACGATATGATGCATTTTAATGATTGTTTCATAAGGAACAg                 >  1:233314/1‑140 (MQ=255)
                                                                                                                  gATTATTTCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAg                 >  1:661374/1‑140 (MQ=255)
                                                                                                                        ttCACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGt           <  2:661374/140‑1 (MQ=255)
                                                                                                                          cACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTaa         <  1:82051/140‑1 (MQ=255)
                                                                                                                          cACCCTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTaa         <  2:171715/140‑1 (MQ=255)
                                                                                                                            cccTCCGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTAAGa       >  1:120181/1‑140 (MQ=255)
                                                                                                                                 cGGGAGCGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTAAGATCTaa  >  1:850452/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAAGGTGCTCAGCAATACATAACCGAGCAGTGTTTAAAGTAAAATCTTAACTACAATATGATTCATTTTGATGATTGTTTCATAAGGAACAGTGAAGTAAGATCTAA  >  NC_000913/1423441‑1423709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑