Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,440,632	T→C	T51T (ACA→ACG)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,440,632	0	T	C	100.0%	183.9 / NA	53	T51T (ACA→ACG)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTA  >  NC_000913/1440497‑1440767                                                                                                                                        |                                                                                                                                        cAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgcgtgt                                                                                                                                     <  2:636199/140‑1 (MQ=255)       gTACAGCGTCGGGATCATCAGCAGCTAACGCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTcccc                                                                                                                               <  1:308626/140‑1 (MQ=255)            gCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACg                                                                                                                          <  1:581515/140‑1 (MQ=255)             cGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCAGCCGCTTCCGACTACATATCAACCACGCGTGGCGTGTCTCCCCAAACGt                                                                                                                         >  2:225465/1‑140 (MQ=255)                  ggATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCttt                                                                                                                    >  2:18775/1‑140 (MQ=255)                     tcatcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAg                                                                                                                 <  1:624394/140‑1 (MQ=255)                      catcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGc                                                                                                                <  2:426937/140‑1 (MQ=255)                      catcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGc                                                                                                                <  1:90745/140‑1 (MQ=255)                       atcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGcc                                                                                                               >  1:546073/1‑140 (MQ=255)                           gcagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGttt                                                                                                           <  2:439544/140‑1 (MQ=255)                            cagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTc                                                                                                          <  1:145189/140‑1 (MQ=255)                            cagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTc                                                                                                          >  2:643104/1‑140 (MQ=255)                             agcaAACCCTGTGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTcc                                                                                                         >  1:430217/1‑140 (MQ=255)                               caAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCgg                                                                                                       >  1:440995/1‑140 (MQ=255)                                 aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGcc                                                                                                     >  1:756811/1‑140 (MQ=255)                                 aaCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGcc                                                                                                     >  1:209520/1‑140 (MQ=255)                                  aCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGccc                                                                                                    <  1:653443/140‑1 (MQ=255)                                    ccTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAg                                                                                                  >  2:206620/1‑140 (MQ=255)                                      tGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAAGCGTGGCGGGTCTCACGTAACGTTCGTTAATACGTTTCCAGCCCAGGc                                                                                                >  2:418283/1‑140 (MQ=255)                                       gCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCa                                                                                               <  2:137395/140‑1 (MQ=255)                                       gCGATGACGTAAACGATCTTGACAGGGCACCCGTCTGCACAGCGCCATCCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCa                                                                                               <  2:235639/140‑1 (MQ=255)                                            gACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGc                                                                                          <  2:209520/140‑1 (MQ=255)                                             aCGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGcc                                                                                         >  1:620219/1‑140 (MQ=255)                                              cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGccc                                                                                                    <  1:490568/128‑1 (MQ=255)                                              cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGccc                                                                                                    >  2:490568/1‑128 (MQ=255)                                              cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCt                                                                                        >  1:134580/1‑140 (MQ=255)                                              cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCt                                                                                        >  1:518284/1‑140 (MQ=255)                                                     gATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGc                                                                                 <  2:440995/140‑1 (MQ=255)                                                       tGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTACGCCATTTCCGGCGCAGGCATCAGCCTGTTCTGCCa                                                                               >  2:285255/1‑140 (MQ=255)                                                               gggCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGaa                                                                       <  1:19572/140‑1 (MQ=255)                                                                 gCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGaa                                                                       >  1:627693/1‑138 (MQ=255)                                                                 gCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGaa                                                                       <  2:627693/138‑1 (MQ=255)                                                                 gCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACt                                                                     <  1:636437/140‑1 (MQ=255)                                                                 gCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTACCCAAACGTTCTTTAAGCCGTTTCCGGCACAGGCATCAGCCTGTTCTGCCATCGTGGAACt                                                                     <  1:545414/140‑1 (MQ=255)                                                                 gCACCCGTCTGCAAAACGCCATCCACGGTCGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACt                                                                     <  1:225465/140‑1 (MQ=255)                                                                   aCCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTgg                                                                   >  2:139522/1‑140 (MQ=255)                                                                    cccGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTggg                                                                  <  2:163776/140‑1 (MQ=255)                                                                        tCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTa                                                              >  1:668108/1‑140 (MQ=255)                                                                        tCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTa                                                              <  2:134580/140‑1 (MQ=255)                                                                        tCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTa                                                              <  1:18775/140‑1 (MQ=255)                                                                                 cgcCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTggggg                                                                <  2:695888/129‑1 (MQ=255)                                                                                 cgcCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTggggg                                                                >  1:695888/1‑129 (MQ=255)                                                                                 cgcCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCACCGGGGAACTGGGGGTAATAGGGTAg                                                     >  1:335031/1‑140 (MQ=255)                                                                                    cATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATg                                                  >  2:11241/1‑140 (MQ=255)                                                                                           ggTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATATGGTAGATGGCGATaa                                           >  2:606203/1‑140 (MQ=255)                                                                                                     gCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGt                                 >  1:604155/1‑140 (MQ=255)                                                                                                      cACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTg                                <  1:643104/140‑1 (MQ=255)                                                                                                           gCTTCCGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGaaa                           >  1:206990/1‑140 (MQ=255)                                                                                                               ccGACTGCATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCg                       <  1:11241/140‑1 (MQ=255)                                                                                                                      cATTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAg                >  1:451382/1‑140 (MQ=255)                                                                                                                       aTTTCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGc               >  1:147400/1‑140 (MQ=255)                                                                                                                         ttCAACCACGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCaa             >  1:92131/1‑140 (MQ=255)                                                                                                                                aCGCGTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGcc      >  2:11867/1‑140 (MQ=255)                                                                                                                                 cgcgTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCa     <  2:668108/140‑1 (MQ=255)                                                                                                                                   cgTGGCGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCAtt   <  1:45670/140‑1 (MQ=255)                                                                                                                                    gtggcgTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTa  <  2:206990/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTA  >  NC_000913/1440497‑1440767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑