Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,078,054 (A)5→4 coding (606/990 nt) yiiD → putative acetyltransferase YiiD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,078,0500A.99.1% 441.9 / ‑3.3 107coding (602/990 nt)yiiDputative acetyltransferase YiiD
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (53/53);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTGATGATTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGG  >  NC_000913/4077914‑4078185
                                                                                                                                        |                                                                                                                                       
ttGATGATTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGCaaaa                                                                                                                                      <  2:1047795/140‑1 (MQ=255)
    tgatTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGCaaaatt                                                                                                                                    >  1:1653218/1‑136 (MQ=255)
    tgatTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGCaaaatt                                                                                                                                    <  2:1653218/138‑3 (MQ=255)
      atTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATc                                                                                                                               <  2:1637547/140‑1 (MQ=255)
        tAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCAc                                                                                                                             >  2:1470177/1‑140 (MQ=255)
        tAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCAc                                                                                                                             >  1:468751/1‑140 (MQ=255)
          aGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTa                                                                                                                           <  2:1277162/140‑1 (MQ=255)
             ccGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCa                                                                                                                        <  1:174758/140‑1 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGGATTGAGCGATATACCGAGC‑AAGATTTATCACTACCATGc                                                                                                                     >  2:503599/1‑140 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGc                                                                                                                     >  1:1755689/1‑140 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGc                                                                                                                     >  2:1790378/1‑140 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGc                                                                                                                     >  2:1458683/1‑140 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGc                                                                                                                     >  2:1389180/1‑140 (MQ=255)
                tCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGc                                                                                                                     >  2:384949/1‑140 (MQ=255)
                 cGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGcc                                                                                                                    <  2:1239991/140‑1 (MQ=255)
                 cGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGcc                                                                                                                    >  2:1129198/1‑140 (MQ=255)
                           gATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGc                                                                                                          <  1:1470177/140‑1 (MQ=255)
                               aCATTCTGCATCGCGTCGACTGTTGTGCGCAGCTGCACCAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      <  1:503599/140‑1 (MQ=255)
                               aCATTCTGCATCGCGCCGACTGGTGAGCGGAGCTGCATCAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      <  1:538719/140‑1 (MQ=255)
                                 aTTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAg                                                                                                    <  1:384949/140‑1 (MQ=255)
                                 aTTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGAGC‑AGAATGTATCACTAGCATGCCAGAAACCGGCAATCAg                                                                                                    >  2:538719/1‑140 (MQ=255)
                                  ttCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGa                                                                                                   <  2:468751/140‑1 (MQ=255)
                                   tCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGaa                                                                                                  >  1:1113712/1‑140 (MQ=255)
                                   tCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGaa                                                                                                  >  1:251427/1‑140 (MQ=255)
                                    cTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAAt                                                                                                 <  1:1652909/140‑1 (MQ=255)
                                    cTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAAt                                                                                                 <  1:1129198/140‑1 (MQ=255)
                                    cTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAAt                                                                                                 <  2:422998/140‑1 (MQ=255)
                                     tGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGTGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      >  2:1798537/1‑134 (MQ=255)
                                     tGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGTGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      <  1:1798537/134‑1 (MQ=255)
                                     tGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATc                                                                                                >  2:119499/1‑140 (MQ=255)
                                     tGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATc                                                                                                >  1:1011859/1‑140 (MQ=255)
                                     tGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATA‑CGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATcc                                                                                               <  1:990722/140‑1 (MQ=255)
                                      gCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCa                                                                                                         <  2:1337753/130‑1 (MQ=255)
                                      gCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCa                                                                                                         >  1:1337753/1‑130 (MQ=255)
                                      gCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATcc                                                                                               <  1:1336417/140‑1 (MQ=255)
                                       cATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGTAATCAGAATCCg                                                                                              <  1:1458683/140‑1 (MQ=255)
                                       cATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCg                                                                                              <  1:1389180/140‑1 (MQ=255)
                                        aTCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGc                                                                                             <  2:75405/140‑1 (MQ=255)
                                          cgcgGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      <  1:1812960/129‑1 (MQ=255)
                                          cgcgGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATc                                                                                                      >  2:1812960/1‑129 (MQ=255)
                                          cgcgGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCg                                                                                              >  1:1347721/1‑137 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGa                                                                                                   <  1:149754/130‑1 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGa                                                                                                   >  2:149754/1‑130 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCa                                                                                            <  2:266586/137‑1 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCa                                                                                            >  1:266586/1‑137 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCa                                                                                         >  2:135793/1‑140 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTACCACTACCATGCCAGAAACCGGCAATc                                                                                                      <  1:1123545/127‑1 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTACCACTACCATGCCAGAAACCGGCAATc                                                                                                      >  2:1123545/1‑127 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTAACACTACCTTGCCAGAAGCCGGCAATCAGAATCCGCACCa                                                                                         >  2:689231/1‑140 (MQ=255)
                                            cggcgACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGG‑AAAAATTTATCACAACCGTGCCAGAAACCGGGAATCAGAATCCGCACCa                                                                                         >  2:721669/1‑140 (MQ=255)
                                                         gcgcAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGc                                                                                             >  2:156920/1‑123 (MQ=255)
                                                            cAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCg                                                                           >  1:1126774/1‑138 (MQ=255)
                                                            cAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCg                                                                           <  2:1126774/138‑1 (MQ=255)
                                                            cAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCg                                                                           <  2:397462/138‑1 (MQ=255)
                                                            cAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCg                                                                           >  1:397462/1‑138 (MQ=255)
                                                                 gCAACAGTCGTGGTACGAACATGTCTCGCGTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGttt                                                                    <  1:761342/140‑1 (MQ=255)
                                                                  cAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTa                                                                   <  2:1283906/140‑1 (MQ=255)
                                                                    aCAGGCGTGGTACGAACATATCTCGGTTAGTGAAAAACTGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTAtt                                                                 <  1:721669/140‑1 (MQ=255)
                                                                    aCAGGAGTGATCCGCACATAACCCGCCTAGCGAAAAAATGGGAGTGGGCATTCAGCAATATACCGAGC‑AAACTCTATATCTGTCACGCCACAAGCCGGCAATCAGCATCAGCCCCATACGCTGTTTGCCGCGAGTTTAtt                                                                 <  2:1885217/140‑1 (MQ=255)
                                                                       ggCGTGGTACGAACATATCCCGCTTCGTGGAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATtctc                                                              <  1:921015/140‑1 (MQ=255)
                                                                          gTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCa                                                             <  1:854687/138‑1 (MQ=255)
                                                                          gTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCa                                                             >  2:854687/1‑138 (MQ=255)
                                                                           tGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTg                                                          <  1:1111290/140‑1 (MQ=255)
                                                                               aCGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGa                                                      <  1:411010/140‑1 (MQ=255)
                                                                               aCGAACATATCCCACTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGa                                                      <  1:81934/140‑1 (MQ=255)
                                                                                   aCATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCt                                                  <  2:1011859/140‑1 (MQ=255)
                                                                                      tatCCCGCTTAGTGATAAAATGTGCGTGCACATTCAGTAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCAc                                               <  1:853918/140‑1 (MQ=255)
                                                                                      tatCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCAc                                               <  2:1113712/140‑1 (MQ=255)
                                                                                       atCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACg                                                    >  1:1474623/1‑134 (MQ=255)
                                                                                       atCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACg                                                    <  2:1474623/134‑1 (MQ=255)
                                                                                       atCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACt                                              >  1:417252/1‑140 (MQ=255)
                                                                                        tCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATTAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTg                                             >  2:1154815/1‑140 (MQ=255)
                                                                                        tCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTGTTCTTTCTGGCGATGCTCACTg                                             >  2:853918/1‑140 (MQ=255)
                                                                                        tCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCg                                                       <  1:433076/130‑1 (MQ=255)
                                                                                        tCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTg                                             >  2:1689838/1‑140 (MQ=255)
                                                                                           cGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATGCCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCCCCATACGCTGTTTGCCGGGAGTTTATTCCCACTGGCGACGCTCACTGGtt                                          <  1:1787816/140‑1 (MQ=255)
                                                                                            gCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCCTACGCTGTGTGCAGGGAGTTTATTCGCACTAGCGACGCTCACTGGTTg                                         >  2:706564/1‑140 (MQ=255)
                                                                                            gCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTg                                         <  1:119499/140‑1 (MQ=255)
                                                                                             cTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTgg                                        >  2:1582267/1‑140 (MQ=255)
                                                                                             cTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTgg                                        >  1:998557/1‑140 (MQ=255)
                                                                                             cTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTgg                                        >  2:1427067/1‑140 (MQ=255)
                                                                                              ttAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTggg                                       >  2:13132/1‑140 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCa                                                <  1:1737217/130‑1 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCa                                                >  2:1737217/1‑130 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGtt                                          <  2:1487114/136‑1 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGtt                                          >  1:1487114/1‑136 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTgggg                                      <  2:251427/140‑1 (MQ=255)
                                                                                               tAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCAATGGCGACGCTCACTGGTTgggg                                      >  1:608636/1‑140 (MQ=255)
                                                                                                aGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGa                                     >  2:305410/1‑140 (MQ=255)
                                                                                                      aaaaTGGGCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCAGTGGGGAGGCTCACGGGTTGGGGACTTATc                               >  1:577126/1‑140 (MQ=255)
                                                                                                            ggCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTg                         >  1:1050842/1‑140 (MQ=255)
                                                                                                            ggCGTGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTg                         >  2:191754/1‑140 (MQ=255)
                                                                                                                tGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGAtgc                     <  1:1689838/140‑1 (MQ=255)
                                                                                                                tGCGCATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGAtgc                     >  1:1269833/1‑140 (MQ=255)
                                                                                                                tGCGCATTCAGCAATATACCGGAC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGAtgc                     >  2:1369539/1‑140 (MQ=255)
                                                                                                                   gcATTCAGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGAtgctgc                  <  2:1269833/140‑1 (MQ=255)
                                                                                                                         aGCAATATACCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAAc            <  2:1050842/140‑1 (MQ=255)
                                                                                                                                 aCCGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTc    >  2:1148919/1‑140 (MQ=255)
                                                                                                                                   cGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTcgg  >  2:207804/1‑140 (MQ=255)
                                                                                                                                   cGGGC‑AAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTcgg  >  1:1119298/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTGATGATTAAGCCCGTCGCCACTCTGGATGACATTCTGCATCGCGGCGACTGGTGCGCGCAGCTGCAACAGGCGTGGTACGAACATATCCCGCTTAGTGAAAAAATGGGCGTGCGCATTCAGCAATATACCGGGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGG  >  NC_000913/4077914‑4078185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑