BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,001,304	(G)_7→8	coding (960/2259 nt)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,297	1	.	G	97.4%	403.1 / 1.5	115	coding (953/2259 nt)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (53/57); total (57/58)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 130 total aligned reads displayed.

TCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGT  >  NC_000913/3001159‑3001431

tCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACTGGCGCTTTTGCATCTCGCGGGCACTCCATCGCTTCTG‑C‑‑g                                                                                                                                          >  2:608209/1‑140 (MQ=255)

 cGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑gg                                                                                                                                         >  2:1495038/1‑140 (MQ=255)

   cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAAAACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g                                                                                                                                      >  1:1066362/1‑140 (MQ=255)

   cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCAAACACCGGAGCTCAAGCCTCTCACGAGCACCCCATCGCTCCTG‑C‑‑ggg‑g                                                                                                                                      >  2:877072/1‑140 (MQ=255)

    accccGTCCCCGCCATGCTTTTTCCATTGGCGGGCAATTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g‑g                                                                                                                                    <  1:570118/138‑1 (MQ=255)

    accccGCACCCGCCATGCTTTTGCCGTTGCCGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g‑g                                                                                                                                    <  1:893284/138‑1 (MQ=255)

    aaCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g‑g                                                                                                                                    <  1:962469/140‑1 (MQ=255)

           aCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g‑gg‑ggaata                                                                                                                            <  2:321340/140‑6 (MQ=255)

              cGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAg                                                                                                                          >  1:1855656/1‑140 (MQ=255)

                ccACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTTAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGTG‑‑GGG‑G‑GG‑GAATAAAGTc                                                                                                                        >  1:1905074/1‑140 (MQ=255)

                ccACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTc                                                                                                                        >  2:478228/1‑140 (MQ=255)

                 cACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑ggg‑g‑g                                                                                                                                    >  1:952995/1‑127 (MQ=255)

                 cACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACGCCATCGCTTCTG‑CA‑GGG‑G‑GG‑GAATAAAGTCg                                                                                                                       >  2:724170/1‑140 (MQ=255)

                  aCGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGc                                                                                                                      <  2:1775712/140‑1 (MQ=255)

                   cGCTTTTACCATTGACGGGCAAGTGGGCGTGAACCGCCACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCt                                                                                                                     <  1:843876/140‑1 (MQ=255)

                   cGCTTTTACCATTGACGGGCAAATGGGCGTGACCCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCGCTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCt                                                                                                                     <  1:1690771/140‑1 (MQ=255)

                    gCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGCGCACTCCTTCGCTTCTT‑C‑‑GGGAG‑GG‑GAATAAAGTCGCtt                                                                                                                    >  2:790589/1‑140 (MQ=255)

                      ttttACCATTGACGGGCGAATGCGCGTGAACGGCGCCGGAACATTGAATGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTAc                                                                                                                  <  2:749697/140‑1 (MQ=255)

                             aTTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATc                                                                                                           <  1:1652514/140‑1 (MQ=255)

                                gACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTc                                                                                                        >  2:1615440/1‑140 (MQ=255)

                                  cGGGCAAATGGGCGTCAACCGCTACTGAACATTGAAACGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGt                                                                                                      <  1:875796/140‑1 (MQ=255)

                                      cAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgtg                                                                                                  <  2:1855656/140‑1 (MQ=255)

                                        aaTGGGCGTGAACCGCGACGGACCATTGAAAGGTTCTAGTCTGGATGTCCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                                                                <  2:571254/140‑1 (MQ=255)

                                        aaTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTGCCTTTATCCTCGTTGTGcc                                                                                                <  1:1835673/140‑1 (MQ=255)

                                        aaTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                                                                <  1:316028/140‑1 (MQ=255)

                                         aTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                                                                >  1:1369412/1‑139 (MQ=255)

                                         aTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                                                                <  2:1369412/139‑1 (MQ=255)

                                         aTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCt                                                                                               >  1:468159/1‑140 (MQ=255)

                                         aTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑GGG‑GAGG‑GAATAAAGTGGCGTACCTTGATCCTCGTTGTGCCt                                                                                               >  1:571254/1‑140 (MQ=255)

                                           gggCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTAc                                                                                             <  2:1703787/140‑1 (MQ=255)

                                            ggCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCATACCTTTATCCTCGTTGTGCCTACg                                                                                            >  1:754603/1‑140 (MQ=255)

                                                tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTtgtg                                                                                                  <  1:170185/130‑1 (MQ=255)

                                                tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                                                                        <  2:468159/140‑1 (MQ=255)

                                                tGAACCGCGACGGAACATTGAAAGGGTATTGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                                                                        <  1:839507/140‑1 (MQ=255)

                                                 gAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTAc                                                                                       <  2:1588241/140‑1 (MQ=255)

                                                  aaCCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACa                                                                                      <  1:1603/140‑1 (MQ=255)

                                                    ccGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGt                                                                                    >  1:1610949/1‑140 (MQ=255)

                                                    ccGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGACGCTTACATTTGTCATCGTTGCGCCTACGCTTACAGt                                                                                    >  1:621146/1‑140 (MQ=255)

                                                       cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTc                                                                                  >  1:1571748/1‑140 (MQ=255)

                                                       cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa                                                                                 >  2:1674363/1‑140 (MQ=255)

                                                       cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa                                                                                 >  1:1597698/1‑140 (MQ=255)

                                                          cGGAACATTGAAAGGTTATAGTTTGGATGTTTTATCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                                                              <  2:859131/140‑1 (MQ=255)

                                                          cGGAACATTGAAAGGTTATAGTCTGGATGTTTTGTTTAACACCGGCGCCTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                                                              <  2:754603/140‑1 (MQ=255)

                                                          cGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                                                              <  1:478228/140‑1 (MQ=255)

                                                          cGGAACATTGAAAGGATATAGTCTGGGTGTTCTGTCTGACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                                                              <  1:827968/140‑1 (MQ=255)

                                                           ggAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa                                                                             <  1:1674363/140‑1 (MQ=255)

                                                            gAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGAc                                                                            <  1:1615440/140‑1 (MQ=255)

                                                               cATTGAAAGGTTATACTCTGGAGGTCCTGACTAACACGGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg                                                                         <  1:608209/140‑1 (MQ=255)

                                                                 ttGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCt                                                                       <  2:1597698/140‑1 (MQ=255)

                                                                    aaaGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg                                                                         >  1:1566360/1‑135 (MQ=255)

                                                                    aaaGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg                                                                         <  2:1566360/135‑1 (MQ=255)

                                                                      aGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATAcc                                                                  >  2:1216273/1‑140 (MQ=255)

                                                                           ataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCt                                                             >  2:1684415/1‑140 (MQ=255)

                                                                           ataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCt                                                             >  1:479037/1‑140 (MQ=255)

                                                                           ataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTACCTACGCTTACGGTGCAAAGACCTGCTATACCAACCt                                                             >  1:671667/1‑140 (MQ=255)

                                                                              gTCTGGATGTTCTGTCTTACACTGGCGCTTACGCAACTCCCGGGCACTACATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc                                                          <  2:671667/140‑1 (MQ=255)

                                                                                 tGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc                                                              >  2:347079/1‑133 (MQ=255)

                                                                                 tGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc                                                              <  1:347079/133‑1 (MQ=255)

                                                                                  ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTcccc                                                         >  2:1721522/1‑137 (MQ=255)

                                                                                  ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTcccc                                                         <  1:1721522/137‑1 (MQ=255)

                                                                                   gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg                                                     >  2:1364174/1‑140 (MQ=255)

                                                                                   gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTGGTGCCTACACTTGCAGTTCAAAGACCTGCTGTACCAACCTCCCCTCgg                                                     >  2:789335/1‑140 (MQ=255)

                                                                                        tCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGc                                                    <  2:1803059/136‑1 (MQ=255)

                                                                                        tCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGc                                                    >  1:1803059/1‑136 (MQ=255)

                                                                                           gTCTAACACCGGCGCTAATGCATCTCACGGGCAGTTCATGGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCg                                             <  2:834790/140‑1 (MQ=255)

                                                                                             cTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGAt                                           >  1:1754433/1‑140 (MQ=255)

                                                                                              tAACACCGGCGCTTATGGATCTGACGGGCACTCCACCGCTTCTG‑C‑‑GGGTG‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg                                          <  2:619935/140‑1 (MQ=255)

                                                                                              tAACACCGGCGCTTATGCATCTCACGGGCGCTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg                                          <  1:1516022/140‑1 (MQ=255)

                                                                                                acacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCg                                        <  2:1754433/140‑1 (MQ=255)

                                                                                                 cacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCATCCCCCCCTCTGCTGGTGCGATGCGt                                       >  1:799120/1‑140 (MQ=255)

                                                                                                      gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATCCCAACCTCCCTTCGGGTGGTGCGATGCGTGGtt                                   >  2:823392/1‑140 (MQ=255)

                                                                                                      gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  2:1600460/1‑140 (MQ=255)

                                                                                                      gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  1:1383565/1‑140 (MQ=255)

                                                                                                      gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAACTCCCCACGGCTGGTGCGATGCGTGGTTa                                  >  1:834790/1‑140 (MQ=255)

                                                                                                          ttATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgc                              <  1:1323914/140‑1 (MQ=255)

                                                                                                           tATGCATCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgcg                             <  2:479037/140‑1 (MQ=255)

                                                                                                                aTCTCACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAAAAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCca                        <  1:724170/140‑1 (MQ=255)

                                                                                                                  ctcACGGGCACTCCACCGCTTCGG‑CCGGGG‑G‑GG‑GAATGAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCcac                       <  1:823392/140‑1 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGTGATGCGTGGTTATGGCGCGCCACAAg                    >  1:1522537/1‑140 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgcg                             <  2:118048/131‑1 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgcg                             >  1:118048/1‑131 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    <  1:1364174/140‑1 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    >  1:1228391/1‑140 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    >  2:239836/1‑140 (MQ=255)

                                                                                                                    cACGGGCACTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    >  1:1133195/1‑140 (MQ=255)

                                                                                                                            cTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTAttt            >  2:239510/1‑140 (MQ=255)

                                                                                                                            cTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTAttt            >  2:1655727/1‑140 (MQ=255)

                                                                                                                            cTCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTAttt            >  1:127099/1‑140 (MQ=255)

                                                                                                                             tCCATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTg           >  1:1703951/1‑140 (MQ=255)

                                                                                                                              ccATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGc          <  1:117444/140‑1 (MQ=255)

                                                                                                                               cATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGcc         >  2:117655/1‑140 (MQ=255)

                                                                                                                               cATCGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGcc         >  2:1424051/1‑140 (MQ=255)

                                                                                                                                  cGCTTCTG‑CG‑GGG‑G‑GG‑GAATAACGTCGCTCACCTTTAACCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  1:650267/140‑1 (MQ=255)

                                                                                                                                  cGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      >  1:148790/1‑140 (MQ=255)

                                                                                                                                  cGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  1:1495038/140‑1 (MQ=255)

                                                                                                                                  cGCTTCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  2:148790/140‑1 (MQ=255)

                                                                                                                                  cGCTTCTG‑C‑‑GGG‑G‑GGCGAATAATGTAGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  1:790589/140‑1 (MQ=255)

                                                                                                                                  cGCTTCTG‑C‑‑GGG‑G‑GGCGAATAAGGTAGCTTTCCTTTAGCCTCGTTGTGCCTACGCTTACAGTACAAAGGCCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCCCCACAAGTCGTATTTGCCGtt      <  1:788148/140‑1 (MQ=255)

                                                                                                                                      tCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGt  <  1:239836/140‑1 (MQ=255)

                                                                                                                                      tCTG‑CG‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGt  <  2:1436259/140‑1 (MQ=255)

TCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑‑GGG‑G‑GG‑GAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGT  >  NC_000913/3001159‑3001431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`