Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,525	C→T	R98C (CGC→TGC)	yaaX →	DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,525	0	C	T	100.0%	227.3 / NA	74	R98C (CGC→TGC)	yaaX	DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/39);  total (35/39)

CGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACG  >  NC_000913/5389‑5658                                                                                                                                         |                                                                                                                                       cgACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCt                                                                                                                                     >  1:428594/1‑140 (MQ=255)   aCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTaa                                                                                                                                   <  2:1439628/140‑1 (MQ=255)   aCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTaa                                                                                                                                   <  1:1737487/140‑1 (MQ=255)   aCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTaa                                                                                                                                   >  1:346666/1‑140 (MQ=255)   aCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTaa                                                                                                                                   >  1:1187875/1‑140 (MQ=255)    ccACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATGATCACGGCGGTCATGGGCCCGACAAACAACACTGcctgt                                                                                                                                  >  2:702107/1‑136 (MQ=255)        ggCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGAc                                                                                                                              <  2:1659063/140‑1 (MQ=255)             gtggAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATg                                                                                                                        <  2:1187875/140‑1 (MQ=255)             gtggAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATg                                                                                                                        <  2:942015/140‑1 (MQ=255)              tggAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCCTCACGGCGGGCATGGTGCAGGCAAAGATCAATGCTAAATGACAA‑ATGc                                                                                                                       >  2:555965/1‑140 (MQ=255)              tggAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGc                                                                                                                       >  1:1469948/1‑140 (MQ=255)              tggAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGc                                                                                                                       >  1:1872612/1‑140 (MQ=255)                       caTTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACa                                                                                                              >  2:237961/1‑140 (MQ=255)                            tGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATAGTCCAGGCTAACATCACTGCTAAATCACAA‑AAGCCGGGGAACAATCCg                                                                                                         >  2:589061/1‑140 (MQ=255)                               aTGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACACCACTGCTAAATTACAA‑ATGCCCGGTAACAATCCGGCa                                                                                                      >  1:732463/1‑140 (MQ=255)                                 ggCGAGGCAATCGCTGGCACCTCCAAGGATCGCCGCCCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCAtt                                                                                                    <  1:833678/140‑1 (MQ=255)                                 ggCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCCCGGGCCGCGCAAACAATACCGGTAAATCACAAGAAGCAGGG‑AACAATGCGGccgt                                                                                                    >  2:710690/1‑137 (MQ=255)                                 ggCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTTCTAAAAGACAA‑AAGCCCGTTAACAATCCGGCCtt                                                                                                    >  1:915741/1‑140 (MQ=255)                                 ggCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCAtt                                                                                                    >  2:1523051/1‑140 (MQ=255)                                 ggCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCCGGCAAACAACACGGCTAAATGACAA‑GAGCCCAGGAAAAATCCGGCAtt                                                                                                    >  2:833678/1‑140 (MQ=255)                                  gCGAGGCAATCGCTGTCACCTAAACAGACCGCCGCCATCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTc                                                                                                   <  1:555965/140‑1 (MQ=255)                                   cGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCAGGCAAACATCTCTGGTAAGTGACAA‑ATGCCGGTGAACAATCCGGCATTCa                                                                                                  >  2:543840/1‑140 (MQ=255)                                      ggCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAgcg                                                                                               >  1:1085116/1‑140 (MQ=255)                                       gCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAgcgc                                                                                              <  1:22124/140‑1 (MQ=255)                                            cGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGAt                                                                                         >  1:1078281/1‑140 (MQ=255)                                                   aCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGc                                                                                  >  2:103378/1‑140 (MQ=255)                                                      tACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTgg                                                                               <  1:1707516/140‑1 (MQ=255)                                                      tACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTgg                                                                               <  1:1523051/140‑1 (MQ=255)                                                      tACACGGACCGCCGCCACCGCCCCGCCACTATAAGAATGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTgg                                                                               <  1:589061/140‑1 (MQ=255)                                                         acGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGgcgc                                                                            <  2:1388397/140‑1 (MQ=255)                                                          cGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGcgcg                                                                           <  1:388675/140‑1 (MQ=255)                                                            gACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTc                                                                         >  2:241758/1‑140 (MQ=255)                                                             aCCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCt                                                                        >  1:1329607/1‑140 (MQ=255)                                                             aCCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCt                                                                        >  2:338381/1‑140 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  2:1872612/140‑1 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  2:1569733/140‑1 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  2:1469948/140‑1 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  1:1370279/140‑1 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  2:1317168/140‑1 (MQ=255)                                                                gccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     >  1:157198/1‑140 (MQ=255)                                                                cccgccACCGCCGCGGCACCATGAGAAAGCCCCTCATAATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                     <  1:710690/139‑1 (MQ=39)                                                                 ccgccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATc                                                                    >  1:1715545/1‑140 (MQ=255)                                                                    ccACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGTGACGCTGGCGCGTCTTATCAgg                                                                 <  1:345257/140‑1 (MQ=255)                                                                     cACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                <  1:103378/140‑1 (MQ=255)                                                                      aCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                               <  1:338381/140‑1 (MQ=255)                                                                              gcCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa                                                       <  2:428594/140‑1 (MQ=255)                                                                              gcCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa                                                       <  2:150838/140‑1 (MQ=255)                                                                              gcCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa                                                       <  2:1329607/140‑1 (MQ=255)                                                                                  ccaTAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCt                                                   <  1:333820/140‑1 (MQ=255)                                                                                    aTAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCT‑AATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                                <  2:1650312/140‑1 (MQ=255)                                                                                     tAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                                <  1:451605/140‑1 (MQ=255)                                                                                     tAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                                <  2:1449676/140‑1 (MQ=255)                                                                                      aaGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCaa                                               <  1:1835151/140‑1 (MQ=255)                                                                                      aaGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCaa                                               <  2:1715545/140‑1 (MQ=255)                                                                                          aaGCTCCTCATGATCATCACGGCGGTCATGGTCCCGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGGCGGGGCGACTTATCAGGCCTAGGTTAATTCTGCAatata                                           >  2:493599/1‑140 (MQ=255)                                                                                             ctcctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                                <  2:1691589/132‑1 (MQ=255)                                                                                             ctcctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                                >  1:1691589/1‑132 (MQ=255)                                                                                             ctcctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATAttt                                        >  2:1841427/1‑139 (MQ=255)                                                                                             ctcctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACAATCCGGCATTCAGCGCCCGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTg                                        >  2:1038434/1‑140 (MQ=255)                                                                                               cctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCCGCATTCAGCGCCTGATGCGACGCTGGCGCTTCTTATCCGGCTTAAGTTAATGCTGCAATATATTGaa                                      >  1:603966/1‑140 (MQ=39)                                                                                                ctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAAt                                     >  1:1537703/1‑140 (MQ=255)                                                                                                ctcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGACTTATCAGGCCTACTTTAATTTTGCAATATATTGaaa                                     >  1:772510/1‑139 (MQ=255)                                                                                                 tcATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATc                                    >  1:1132171/1‑140 (MQ=255)                                                                                                  cATGATCATCACGGCTGTCATGCTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCt                                   <  1:543840/140‑1 (MQ=255)                                                                                                     gATCATCACGGCGGTCTCGGTCTAGGCAAATATCACTGCCAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa                                <  2:915741/140‑1 (MQ=37)                                                                                                       tcatcaCGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATg                              <  1:271013/140‑1 (MQ=255)                                                                                                        catcaCGGCGGTCATGGGCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTTAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGc                             >  2:1291741/1‑140 (MQ=255)                                                                                                           caCGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACAATCCCGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCttt                          >  2:206664/1‑140 (MQ=255)                                                                                                            aCGGCGGTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCtttt                         <  1:1191434/140‑1 (MQ=255)                                                                                                                cggTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAg                     <  2:346666/140‑1 (MQ=255)                                                                                                                 ggTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg                    <  2:1085116/140‑1 (MQ=255)                                                                                                                  gTCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGc                   >  1:1702681/1‑140 (MQ=255)                                                                                                                   tCATGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCa                  >  1:1591878/1‑140 (MQ=255)                                                                                                                    cATGGGCCAGGCAAACAACACTGCTAAAAGAAAA‑AAGCCGGGGAACAATCCGGCATTCATCGCCCGAATCGACGCTGGCGCGTCTTATCAGGGCCACGTTAATTCTCCCATATATTTAATCTGTATTCTTTTGTAAGCAg                 >  2:1191434/1‑140 (MQ=255)                                                                                                                     aTGGTCCAGGCAAACATCACTGCTAAATGACAA‑ATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAgg                >  2:1119767/1‑140 (MQ=255)                                                                                                                             ggCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg                    >  2:476553/1‑128 (MQ=255)                                                                                                                             ggCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg                    <  1:476553/128‑1 (MQ=255)                                                                                                                             ggCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCg        >  1:1875077/1‑140 (MQ=255)                                                                                                                             ggCAAACATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCg        >  1:1276231/1‑140 (MQ=255)                                                                                                                                 aaCATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCa    >  1:1733332/1‑140 (MQ=255)                                                                                                                                   cATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcg  >  2:1263475/1‑140 (MQ=255)                                                                                                                                   cATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcg  >  2:1070604/1‑140 (MQ=255)                                                                                                                                   cATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcg  >  1:1058388/1‑140 (MQ=255)                                                                                                                                   cATCACTGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTACAATATATGGAATCTGCATGCTTTTATAGGCAGGATAAGGCGTTCAcg  >  2:631224/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAA‑ATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACG  >  NC_000913/5389‑5658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑