BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,484	T→C	A273A (GCT→GCC)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,484	0	T	C	97.4%	415.1 / NA	115	A273A (GCT→GCC)	talA	transaldolase A
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (55/57); minor base A (0/3); total (55/60)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAAC  >  NC_000913/2579349‑2579618

cACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCGCATACGTTCTCACGCCCAGCCCCCa                                                                                                                                       >  1:889331/1‑140 (MQ=255)

 aCCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCAt                                                                                                                                      <  1:1634387/140‑1 (MQ=255)

     aaCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGc                                                                                                                                  >  1:1402340/1‑140 (MQ=255)

     aaCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGc                                                                                                                                  >  2:191027/1‑140 (MQ=255)

      aCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCTCAGCCCCCATGAGCg                                                                                                                                 <  1:1615695/140‑1 (MQ=255)

          aTCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGc                                                                                                                             >  2:1777657/1‑140 (MQ=255)

           tCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCg                                                                                                                            <  2:261072/140‑1 (MQ=255)

               cGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGt                                                                                                                        >  1:437686/1‑140 (MQ=255)

               cGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGt                                                                                                                        >  2:1510332/1‑140 (MQ=255)

               cGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCACACCTTCTCAGACGTTACCACGCCCCGCCCGCATGAGCGAAGCGGAGt                                                                                                                        >  2:831462/1‑140 (MQ=255)

                        cGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTggg                                                                                                            <  1:933530/140‑1 (MQ=255)

                          gCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAg                                                                                                          <  1:1777657/140‑1 (MQ=255)

                          gCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAg                                                                                                          <  2:1770758/140‑1 (MQ=255)

                          caggCGATCGACTGACACTCGCTCCGAATTTTCTGCAGGCGCTGCAGGGAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAg                                                                                                          <  2:919579/137‑1 (MQ=255)

                            tGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGca                                                                                                        <  1:103811/140‑1 (MQ=255)

                            tGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGca                                                                                                        <  2:498/140‑1 (MQ=255)

                              cGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGcaca                                                                                                      <  1:1455779/140‑1 (MQ=255)

                                aTCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAAt                                                                                                    >  2:1370835/1‑140 (MQ=255)

                                    actgactATCGCACCCAATTTACTGAAGGCGCTGCAGTAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAgg                                                                                                <  1:601431/140‑1 (MQ=255)

                                     ctgactATTGCACCGAACTTTCTGAAGGAGTAGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGa                                                                                               <  2:918003/140‑1 (MQ=255)

                                        actATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGc                                                                                            >  1:1016501/1‑140 (MQ=255)

                                        actATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCAGAGTTCG‑‑‑GTTGGGAGCACAATCAGGactg                                                                                            >  1:919579/1‑137 (MQ=255)

                                           aTCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGAt                                                                                         >  1:1666310/1‑140 (MQ=255)

                                             cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGcaca                                                                                                      <  2:271328/125‑1 (MQ=255)

                                             cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGcaca                                                                                                      >  1:271328/1‑125 (MQ=255)

                                             cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATgg                                                                                       >  2:1554976/1‑140 (MQ=255)

                                             cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAG‑TTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGc                                                                                      <  2:986581/140‑1 (MQ=255)

                                              gccccGAATTTACTGATGGAGCAGCAGGAAAAAATATCCGCAGTGGTAGGTAAATTAATCCCGCCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGc                                                                                      <  2:812668/137‑1 (MQ=255)

                                              gCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGc                                                                                      <  1:191027/140‑1 (MQ=255)

                                                  cGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCTCCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCgg                                                                                    <  1:1686883/138‑1 (MQ=255)

                                                  cGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGTCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTa                                                                                  >  2:1444474/1‑140 (MQ=255)

                                                  cGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCgg                                                                                    >  2:1686883/1‑138 (MQ=255)

                                                  cGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTGGGGCGCACAATCAAGATGCGATGGCGGTa                                                                                  >  1:921066/1‑140 (MQ=255)

                                                    aaTTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGa                                                                                >  1:405395/1‑140 (MQ=255)

                                                        tACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGaaaaa                                                                            <  1:414174/140‑1 (MQ=255)

                                                              aGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTcc                                                                      >  1:1736541/1‑139 (MQ=255)

                                                               ggAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGAAGCGCAAACAGGATGCGATGGGGGTAGAAAAACTGTCTg                                                                     >  2:727649/1‑140 (MQ=255)

                                                                gAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGa                                                                    <  1:1113420/140‑1 (MQ=255)

                                                                gAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGa                                                                    <  2:1666310/140‑1 (MQ=255)

                                                                    tGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGc                                                                >  1:1918834/1‑140 (MQ=255)

                                                                     gCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCa                                                               >  2:1828423/1‑140 (MQ=255)

                                                                     gCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCa                                                               >  2:1539547/1‑140 (MQ=255)

                                                                               gTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGtt                                                     <  1:1539547/140‑1 (MQ=255)

                                                                               gTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGtt                                                     <  2:1023566/140‑1 (MQ=255)

                                                                                 ttCGCCAGTGGTACGTAAATTATTCCCACCTTCTCCGACTTTCCCACGTCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCg                                                   <  2:636155/140‑1 (MQ=255)

                                                                                         tGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGAt                                           <  2:1402340/140‑1 (MQ=255)

                                                                                         tGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGAt                                           >  1:1807320/1‑140 (MQ=255)

                                                                                              cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGTGGTAGAAAAACTGTCTGAAGGCATTGGTCTTTTCGCCGTTGATCAACg                                      >  2:670815/1‑140 (MQ=255)

                                                                                              cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGAt                                           >  1:999200/1‑135 (MQ=255)

                                                                                              cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACg                                      <  1:1280719/140‑1 (MQ=255)

                                                                                              cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACg                                      <  1:1554976/140‑1 (MQ=255)

                                                                                              cGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACg                                      >  1:1257088/1‑140 (MQ=255)

                                                                                                 aaaTTAATTCCACCTTGACAGAGGTTCCCATCCACGGCCACCATGAGCAAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCaa                                   <  2:921066/140‑1 (MQ=255)

                                                                                                   aTTAATCCCACCTTCTCCGACGCTCCCACGCCCAGCCCCAATGAGCGAGGCGGAGTTCCGTTGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCCCCGTTGATCAACGCa                                    <  2:770856/140‑1 (MQ=255)

                                                                                                   aTTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAAc                                 >  1:96937/1‑140 (MQ=255)

                                                                                                   aTTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAAc                                 >  1:1866156/1‑140 (MQ=255)

                                                                                                    ttAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACt                                <  2:1918834/140‑1 (MQ=255)

                                                                                                     tAATCCCACCTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTg                               >  2:1119706/1‑140 (MQ=255)

                                                                                                      aaTCCCACCTTCTCAGACGTTCCCAAGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTgg                              <  1:831462/140‑1 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCCGTTGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCACCATTGATCCACGCAAACTGGAAGa                          >  1:770856/1‑140 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                                    >  1:937223/1‑127 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                                    <  2:937223/127‑1 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                       >  1:1824550/1‑140 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                       >  2:1200350/1‑140 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                       >  2:1114264/1‑140 (MQ=255)

                                                                                                             ccTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCACTCGGCTGCTCGCCGTTTATCAACGCAAACTGGAAGAtct                       >  2:744235/1‑140 (MQ=255)

                                                                                                              cTTCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtctt                      >  2:1688212/1‑140 (MQ=255)

                                                                                                               ttCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                                    <  1:1188957/125‑1 (MQ=255)

                                                                                                               ttCTCAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                                    >  2:1188957/1‑125 (MQ=255)

                                                                                                                tctcAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtcttct                    <  1:370886/140‑1 (MQ=255)

                                                                                                                tctcAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtcttct                    >  1:1271057/1‑140 (MQ=255)

                                                                                                                  tcAGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTg                  >  2:1679715/1‑140 (MQ=255)

                                                                                                                    aGACGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgcc                >  1:224702/1‑140 (MQ=255)

                                                                                                                     gACGTTCCCACGCCCAGCCCCCATGAGCGAATCGGCGTTAC‑‑‑GTTGGGACCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccg               <  2:587657/140‑1 (MQ=255)

                                                                                                                       cGTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccgcc             <  1:1541761/140‑1 (MQ=255)

                                                                                                                       cGTTCCCACGCCCAGCACCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccgcc             <  2:1016501/140‑1 (MQ=255)

                                                                                                                       cGTTCCCACGCCCAGACCCCATGAGCGAAGCGGAGTTGC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccgcc             <  1:744235/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccgcc             <  2:1807344/139‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgccgcc             >  1:1807344/1‑139 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:1807320/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:96937/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  1:32315/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  1:949485/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  1:1679715/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:61088/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCACCCATGAGCCAAGTGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  1:670815/140‑1 (MQ=255)

                                                                                                                        gTTCCCACGCCCAGCACCCATGAGCAAAGTGGAGTTCA‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  1:727649/140‑1 (MQ=255)

                                                                                                                         ttCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:1393138/139‑1 (MQ=255)

                                                                                                                         ttCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            >  1:1393138/1‑139 (MQ=255)

                                                                                                                          tCCCACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTTAGCTACGCAAACGGGAACGTCTTCTTGCCGCCaaa          >  2:847247/1‑140 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:1640158/137‑1 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            >  1:1640158/1‑137 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAAc         >  2:270927/1‑140 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACGGGAAGATCTTCTTGCCGCCAAAc         >  1:374401/1‑140 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTATGTTCGCCGTTGGTCAGCGCAAACTGGAAGGTCTTCTTGCCGCCAAAc         >  2:503367/1‑140 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGACATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            >  1:1776759/1‑137 (MQ=255)

                                                                                                                           cccACGCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGACATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa            <  2:1776759/137‑1 (MQ=255)

                                                                                                                                gCCCAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATaa    >  1:302332/1‑140 (MQ=255)

                                                                                                                                  ccAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAAc  <  2:82637/140‑1 (MQ=255)

                                                                                                                                  ccAGCCCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGACGTTGATCAACGTAAACTGGAAGATCTTCTGGCCGCCAAACTATAAAc  >  2:800375/1‑140 (MQ=255)

CACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCC‑‑‑GTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACTATAAAC  >  NC_000913/2579349‑2579618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`