Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,946,849 G→A A110A (GCC→GCT)  mltA ← membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,946,8490GA100.0% 380.0 / NA 118A110A (GCC→GCT) mltAmembrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/65);  total (53/65)

Only 100 of 120 total aligned reads displayed.

TCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCGGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGATAT  >  NC_000913/2946715‑2946985
                                                                                                                                      |                                                                                                                                        
tcCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCAcc                                                                                                                                     <  1:147268/140‑1 (MQ=255)
  cGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCtt                                                                                                                                   >  2:1660518/1‑140 (MQ=255)
  cGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCtt                                                                                                                                   >  1:115996/1‑140 (MQ=255)
  cGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCtt                                                                                                                                   >  1:1491071/1‑140 (MQ=255)
     aCGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCa                                                                                                                                <  2:1701730/140‑1 (MQ=255)
         gaCGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTg                                                                                                                            >  2:1068139/1‑140 (MQ=255)
                    acCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCAt                                                                                                                    <  1:253028/137‑1 (MQ=255)
                    acCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCAt                                                                                                                    >  2:253028/1‑137 (MQ=255)
                      cACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATg                                                                                                               <  2:115996/140‑1 (MQ=255)
                           tttGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGaa                                                                                                          <  2:379537/140‑1 (MQ=255)
                           tttGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGaa                                                                                                          <  2:274281/140‑1 (MQ=255)
                           tttGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGaa                                                                                                          <  1:21973/140‑1 (MQ=255)
                               gcggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCg                                                                                                            <  2:276227/134‑1 (MQ=255)
                               gcggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCg                                                                                                            >  1:276227/1‑134 (MQ=255)
                                cggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGaa                                                                                                          <  2:1480606/135‑1 (MQ=255)
                                cggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGaa                                                                                                          >  1:1480606/1‑135 (MQ=255)
                                         gTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGc                                                                                            <  1:1731842/140‑1 (MQ=255)
                                                  aTACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCTAGGCATCCATGCCGAACTGGCCCATATTGCGGGTATCAc                                                                                   >  2:735270/1‑140 (MQ=255)
                                                    aCTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCata                                                                                                <  2:1320779/125‑1 (MQ=255)
                                                    aCTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCata                                                                                                >  1:1320779/1‑125 (MQ=255)
                                                    aCTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATAATGCGGGTATCACCg                                                                                 >  1:63901/1‑140 (MQ=255)
                                                    aCTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATACCGAACTGGCGCATATTGCGGGTATCACCg                                                                                 >  2:1693977/1‑140 (MQ=255)
                                                      tGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGcc                                                                               >  2:1792169/1‑140 (MQ=255)
                                                          aCTCGCCCTGGCGGGTATGGCGCGCCTGATTTACCGGGGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcg                                                                           <  1:556317/140‑1 (MQ=255)
                                                           cTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcgc                                                                          >  1:1535699/1‑140 (MQ=255)
                                                           cTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcgc                                                                          >  2:1342034/1‑140 (MQ=255)
                                                            tCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcgcg                                                                         <  2:435935/140‑1 (MQ=255)
                                                               cccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcgc                                                                          >  2:348828/1‑136 (MQ=255)
                                                               cccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTgcgc                                                                          <  1:348828/136‑1 (MQ=255)
                                                                ccgggggggTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGAAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  2:455856/134‑1 (MQ=255)
                                                                ccTGGCGGTTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCGCCGCCTGCGCGCAGc                                                                     <  1:1330287/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCTCGCCTGCATTACCGGCGTGTACTAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  1:735270/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGCATTACCGGAGTGTAATTACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  2:753653/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGAATTACCGGTGTGAAATAACCGGTAATCTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  2:706966/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  2:1594821/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  2:968913/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  1:1068139/140‑1 (MQ=255)
                                                                ccTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGc                                                                     <  1:1236338/140‑1 (MQ=255)
                                                                         tATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCAGATTGCGGTGATGACGGCCTGCACGCAGCCACTCTTGc                                                            >  1:531100/1‑140 (MQ=255)
                                                                         tATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGGTACCCTAGTTGTCAGGACCTTTCATCTTCCAGGCATCCATGCCGAACTTGGGCATATTGCGGGGATCACCCCCTGCGCTCAGCCACTTTTGc                                                            >  2:1330287/1‑140 (MQ=255)
                                                                          aTGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCa                                                           <  2:1491071/140‑1 (MQ=255)
                                                                            ggCGCGCCTGAATTACCTGCGTCTAATAACCCGTATACTCCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCAcc                                                         <  2:531100/140‑1 (MQ=255)
                                                                            ggCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCAcc                                                         <  1:1012473/140‑1 (MQ=255)
                                                                            ggCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACAATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCAcc                                                         <  2:355175/140‑1 (MQ=255)
                                                                              cgcgcCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCa                                                           <  2:57627/136‑1 (MQ=255)
                                                                              cgcgcCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACcgc                                                       <  2:1535699/140‑1 (MQ=255)
                                                                              cgcgcCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACcgc                                                       >  1:1136160/1‑140 (MQ=255)
                                                                               gcgcCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACcgcg                                                      <  1:1623125/140‑1 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  2:1221041/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  2:261936/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  1:1262180/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  2:1206445/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  2:1106985/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  2:88540/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGACTGGGCGCAGCCAATCTTGCACCGCGTta                                                   >  1:543525/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGAATATTGCGGGTATCACAGCCTGCGCGCAGCGACTCTTGCACCGCGTta                                                   >  2:536892/1‑140 (MQ=255)
                                                                                  ccTGAATTACCGGCGTGCAATAACCGGCAAACTGCACGTTACCATAGGTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTta                                                   >  1:470603/1‑140 (MQ=255)
                                                                                   cTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTtat                                                  <  1:88540/140‑1 (MQ=255)
                                                                                   cTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTtat                                                  <  1:148782/140‑1 (MQ=255)
                                                                                   cTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTtat                                                  <  1:1206445/140‑1 (MQ=255)
                                                                                   cTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTtat                                                  <  1:1106985/140‑1 (MQ=255)
                                                                                   cTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGAGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGAGTtat                                                  <  2:1164974/140‑1 (MQ=255)
                                                                                      aaTTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATaaa                                               >  1:98211/1‑140 (MQ=255)
                                                                                      aaTTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATaaa                                               <  1:1693977/140‑1 (MQ=255)
                                                                                        ttACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACa                                             <  1:1221041/140‑1 (MQ=255)
                                                                                          aCCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACAtt                                           >  1:1800582/1‑140 (MQ=255)
                                                                                           ccGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTa                                          <  1:261936/140‑1 (MQ=255)
                                                                                             ggCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTAct                                        <  2:1165158/140‑1 (MQ=255)
                                                                                               cGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTActct                                      >  1:1646857/1‑140 (MQ=255)
                                                                                                 tgtAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTgg                                    >  1:337845/1‑140 (MQ=255)
                                                                                                  gtAATAACCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGt                                   <  2:1800582/140‑1 (MQ=255)
                                                                                                   taataaCCGGTAAACTGGACGTTACCATAGTTGTCAGCCACTTCCATCCGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGtt                                  <  2:782228/140‑1 (MQ=255)
                                                                                                    aataaCCGGTAAACTGCTCGTTCCCATAGTTCTCAACACATTCCATCTTCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTg                                 <  2:543525/140‑1 (MQ=255)
                                                                                                    aataaCCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTg                                 <  1:7797/140‑1 (MQ=255)
                                                                                                       aaCCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCa                              <  2:98211/140‑1 (MQ=255)
                                                                                                       aaCCGGTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCa                              <  2:337845/140‑1 (MQ=255)
                                                                                                            gTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGt                                   >  2:1904638/1‑130 (MQ=255)
                                                                                                            gTAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGt                                   <  1:1904638/130‑1 (MQ=255)
                                                                                                             tAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGa                        <  1:1342034/140‑1 (MQ=255)
                                                                                                             tAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGa                        >  1:9610/1‑140 (MQ=255)
                                                                                                             tAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGGTATGAAAATTACTATGGTTGCCATACAGa                        >  1:782228/1‑140 (MQ=255)
                                                                                                             tAAACTGCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTACACCGCGCTATAAACATTACTCTGGTTGCCATACAGa                        >  1:716725/1‑140 (MQ=255)
                                                                                                                   gCACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGt                  >  2:136102/1‑140 (MQ=255)
                                                                                                                    cACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGAcg                      <  2:970537/135‑1 (MQ=255)
                                                                                                                    cACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGAcg                      >  1:970537/1‑135 (MQ=255)
                                                                                                                    cACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTg                 >  1:1345052/1‑140 (MQ=255)
                                                                                                                    cACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTg                 >  2:1776696/1‑140 (MQ=255)
                                                                                                                    cACGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTg                 >  1:934484/1‑140 (MQ=255)
                                                                                                                     aCGTTACCATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGa                <  1:1333417/140‑1 (MQ=255)
                                                                                                                            cATAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAAtt          >  1:203331/1‑139 (MQ=255)
                                                                                                                            cATAGTTGTCAGCACCTTCCATCTACCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTa         <  2:1244375/140‑1 (MQ=255)
                                                                                                                            cATAGTTGTCAGCACATTCCATCTGCCAGTCATCAATCCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTa         <  1:536892/140‑1 (MQ=255)
                                                                                                                              tAGTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACg       >  2:222161/1‑140 (MQ=255)
                                                                                                                                gTTGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGa     <  2:934484/140‑1 (MQ=255)
                                                                                                                                gTTGTCAGCACCTTCCAACTGCTAGGCATCATTGCAGAACTGGCGCATTTTGCGGGTATCGCCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGa     <  2:743769/140‑1 (MQ=255)
                                                                                                                                 ttGTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGat    >  2:1772470/1‑140 (MQ=255)
                                                                                                                                   gTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTa         <  2:1643654/133‑1 (MQ=255)
                                                                                                                                   gTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTa         >  1:1643654/1‑133 (MQ=255)
                                                                                                                                   gTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGatat  >  1:1874114/1‑140 (MQ=255)
                                                                                                                                   gTCAGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGatat  >  1:1331806/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATAGTTGTCGGCACCTTCCATCTGCCAGGCATCAATGCCGAACTGGCGCATATTGCGGGTATCACCGCCTGCGCGCAGCCACTCTTGCACCGCGTTATAAACATTACTCTGGTTGCCATACAGACGCGGTGACGAATTACGGATAT  >  NC_000913/2946715‑2946985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑