BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,348	G→A	A153V (GCG→GTG)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,348	0	G	A	99.1%	345.3 / NA	111	A153V (GCG→GTG)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (59/51); total (59/52)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGT  >  NC_000913/3123214‑3123478

   gTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTcc                                                                                                                             >  1:1824343/1‑140 (MQ=255)

     ttATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTg                                                                                                                           <  2:291019/140‑1 (MQ=255)

     ttATCAACCACCTTAAACGCCACCACATCCTAATACCTGACGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTg                                                                                                                           <  2:642731/140‑1 (MQ=255)

      tATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCtt                                                                                                                               >  2:1875838/1‑135 (MQ=255)

      tATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCtt                                                                                                                               <  1:1875838/135‑1 (MQ=255)

       aTCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCg                                                                                                                         >  1:217874/1‑140 (MQ=255)

           accaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTGACCGCGTTACGGGTCGAAGCCGCTTGCCAT‑‑GCCCCCTTCCTGCGGcat                                                                                                                     >  2:513270/1‑140 (MQ=255)

           accaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgat                                                                                                                     >  2:1608772/1‑140 (MQ=255)

           accaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgat                                                                                                                     >  1:1311396/1‑140 (MQ=255)

           accaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGACGCTGACCAT‑‑CACCCCTTCCTGCGGgat                                                                                                                     >  1:396625/1‑140 (MQ=255)

            ccaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCGCCGCGTTGAGGATCGTCGCCGCTGACCAT‑‑CGCCCCTTCCTGCGGgatg                                                                                                                    >  2:888826/1‑140 (MQ=255)

              accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgatgat                                                                                                                  >  2:1224765/1‑140 (MQ=255)

              accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgatgat                                                                                                                  >  1:197627/1‑140 (MQ=255)

              accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgatgat                                                                                                                  >  1:39151/1‑140 (MQ=255)

              accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGgatgat                                                                                                                  >  1:1329974/1‑140 (MQ=255)

               ccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGatgatg                                                                                                                 >  1:1620531/1‑140 (MQ=255)

                  tAAACGCCCCCACATCCGGATAGCTGCCGTTTTTCTGCGGTAGAGATTTATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  2:869241/140‑1 (MQ=255)

                  tAAACGCCACCACATGCTGATAGCTACCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  1:888826/140‑1 (MQ=255)

                  tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              >  1:1905956/1‑140 (MQ=255)

                  tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  2:1905956/140‑1 (MQ=255)

                     aCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTg                                                                                                           <  2:217874/140‑1 (MQ=255)

                     aCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTg                                                                                                           >  2:124765/1‑140 (MQ=255)

                         caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              >  2:1082454/1‑133 (MQ=255)

                         caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  1:1082454/133‑1 (MQ=255)

                          accacATCCTGCTAGCTGCCGTTTACCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGatgatg                                                                                                                 <  2:575825/129‑1 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACGCCTTCCTGCGGGatgatg                                                                                                                 >  1:575825/1‑129 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  2:289014/132‑1 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              <  1:1330723/132‑1 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              >  1:289014/1‑132 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCg                                                                                                              >  2:1330723/1‑132 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGta                                                                                                      >  1:1297123/1‑140 (MQ=255)

                          accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGta                                                                                                      >  2:1891655/1‑140 (MQ=255)

                            cacaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACGAT‑‑CACCCGTTCGTGGGGGATGATGTCGCTGCCGtata                                                                                                    >  1:501042/1‑140 (MQ=255)

                            cacaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                                                                                    <  1:1608772/140‑1 (MQ=255)

                            cacaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                                                                                    <  2:1054046/140‑1 (MQ=255)

                              caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                                                                                  <  1:379120/140‑1 (MQ=255)

                              caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                                                                                  <  1:1891655/140‑1 (MQ=255)

                                 ccTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCa                                                                                               <  1:1564055/140‑1 (MQ=255)

                                      tAGCTGCCGTTTTCCAGCGGTAAAGGTTCTTCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTa                                                                                          <  1:659282/140‑1 (MQ=255)

                                         cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACg                                                                                                 <  2:1848291/130‑1 (MQ=255)

                                         cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAg                                                                                       >  1:1473214/1‑140 (MQ=255)

                                          tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACTCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                                                                                      >  1:1024068/1‑140 (MQ=255)

                                          tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                                                                                      >  1:147239/1‑140 (MQ=255)

                                          tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCT‑CGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                                                                                     >  2:1004085/1‑140 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                                                                                  <  2:131731/127‑1 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                                                                                  >  1:131731/1‑127 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                                                                                     >  1:946307/1‑140 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                                                                                     >  2:1659618/1‑140 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGAGGGATGAGGTCACTGCCGTATAACGCATCGTACAGTg                                                                                     >  1:621681/1‑140 (MQ=255)

                                           gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCT‑CGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGa                                                                                    <  1:1004085/140‑1 (MQ=255)

                                            ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGa                                                                                    >  1:1607991/1‑140 (MQ=255)

                                            ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGa                                                                                    >  1:1757747/1‑140 (MQ=255)

                                               ttttCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑cc                                                                                >  1:100938/1‑140 (MQ=255)

                                                tttCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCAGTTTAACGCCTCGTACAGTGAG‑ccc                                                                               >  1:696574/1‑140 (MQ=255)

                                                   ccAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCag                                                                            <  2:147239/140‑1 (MQ=255)

                                                          tAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGt                                                                     <  2:433528/140‑1 (MQ=255)

                                                          tAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGt                                                                     <  2:946307/140‑1 (MQ=255)

                                                          tAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGt                                                                     <  1:1659618/140‑1 (MQ=255)

                                                          tAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGACGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGt                                                                     <  2:396625/140‑1 (MQ=255)

                                                            gagaTTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTc                                                                   >  1:1024770/1‑140 (MQ=255)

                                                                 tCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTcgccgc                                                              >  2:1094790/1‑140 (MQ=255)

                                                                 tCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTcgccgc                                                              >  2:132199/1‑140 (MQ=255)

                                                                  cATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCcgcg                                                             >  2:1410546/1‑140 (MQ=255)

                                                                    tCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGtt                                                           <  2:385980/140‑1 (MQ=255)

                                                                    tCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGtt                                                           <  2:969669/140‑1 (MQ=255)

                                                                    tCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGtt                                                           >  2:1456268/1‑140 (MQ=255)

                                                                    tCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGAGAGCGTTCGCCGCGtt                                                           >  2:15771/1‑140 (MQ=255)

                                                                    tCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCGGCCGTATACCGCATCGCACAGTGAG‑GCCCACCGAGCGTTCGCCGCGtt                                                           >  2:735818/1‑140 (MQ=255)

                                                                       aGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAg                                                        >  1:993846/1‑140 (MQ=255)

                                                                            gagacagaCCCAGGCGATAACCTGCTCACCGTGTTACGGATCGTAGCCACTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGt                                                   <  2:615243/133‑1 (MQ=255)

                                                                                  aaCCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCggg                                             >  2:49470/1‑140 (MQ=255)

                                                                                      cAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGtt                                         <  1:1571969/140‑1 (MQ=255)

                                                                                       aGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTc                                        <  1:1094790/140‑1 (MQ=255)

                                                                                         gCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCa                                       <  2:39151/140‑1 (MQ=255)

                                                                                           gATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTg                                    <  1:1456268/140‑1 (MQ=255)

                                                                                           gATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGAGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTg                                    <  1:15771/140‑1 (MQ=255)

                                                                                           gATAACCTGCTCACCGCGTTGCAGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTg                                    <  1:735818/140‑1 (MQ=255)

                                                                                              aaCCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCg                                 <  2:1297123/140‑1 (MQ=255)

                                                                                              aaCCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCg                                 <  2:1824343/140‑1 (MQ=255)

                                                                                              aaCCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCATTCGCCGAGTTCAGGGCGTAGTGGGCGTTcctgtctc                                 >  1:615243/1‑133 (MQ=255)

                                                                                               aCCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCgg                                >  1:78641/1‑140 (MQ=255)

                                                                                                  tGCTCACCGCGTTGCGGCTCGTTGCCGCTGAGCAT‑‑CGCCTCTTCCTGCAGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAac                             <  2:572528/140‑1 (MQ=255)

                                                                                                      cACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAaccacc                         <  1:1410546/140‑1 (MQ=255)

                                                                                                         cgcgTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAA‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGc                      >  1:1656770/1‑140 (MQ=255)

                                                                                                          gcgTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCt                     <  1:132199/140‑1 (MQ=255)

                                                                                                           cgTTGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCGGCGCGTATCGGACGGTCATTGGCGGAACCACCAGCTg                    >  1:521049/1‑140 (MQ=255)

                                                                                                             ttGCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCg                  <  1:265880/140‑1 (MQ=255)

                                                                                                               gCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCGCCAGCTGCGGc                <  2:1607991/140‑1 (MQ=255)

                                                                                                               gCGGATCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGc                >  2:1675780/1‑140 (MQ=255)

                                                                                                                cGGATCGTAGCCGCTGACCATCACACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCgg                 <  1:983349/140‑1 (MQ=255)

                                                                                                                 ggATCGTAGCCGCTGGCCAT‑‑CACCCGTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGccc              <  1:862980/140‑1 (MQ=255)

                                                                                                                   aTCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCCGAAAGATTTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCg            <  2:854651/140‑1 (MQ=255)

                                                                                                                    tCGTAGCCGCTGACCAT‑‑TACCCCTTCCTGCGCGATGATGCCGCTGCCGCATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGc           <  2:521049/140‑1 (MQ=255)

                                                                                                                    tCGTAGCCGCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGc           <  2:1620531/140‑1 (MQ=255)

                                                                                                                            gCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCctggctgg   >  2:1402230/1‑140 (MQ=255)

                                                                                                                            gCTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCctggctgg   <  1:49470/140‑1 (MQ=255)

                                                                                                                             cTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGt  >  2:1622404/1‑140 (MQ=255)

                                                                                                                             cTGACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTCCATCGCCCGAACCACCAGCTGCGGCCCCGCCTGGCTGGt  >  2:802147/1‑140 (MQ=255)

                                                                                                                               gACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCctggct     >  2:1003098/1‑135 (MQ=255)

                                                                                                                               gACCAT‑‑CACCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCctggct     <  1:1003098/135‑1 (MQ=255)

ATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAT‑‑CGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAG‑CCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGT  >  NC_000913/3123214‑3123478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`