Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,208,832 G→A R270H (CGC→CAC)  ttdT → L‑tartrate:succinate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,8320GA98.4% 195.5 / NA 62R270H (CGC→CAC) ttdTL‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (43/17);  total (43/19)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAG  >  NC_000913/3208695‑3208967
                                                                                                                                         |                                                                                                                                       
cgcATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTATCCGCCGGTACTTAATTCTGGCGATCAGGTGCCGCACt                                                                                                                                       >  1:736274/1‑140 (MQ=255)
     ctcACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTTCTGTACCCGCCGGTACGGAAGTCTGGCGATGAGGTGCCGCACTGGGCa                                                                                                                                  >  2:567032/1‑140 (MQ=255)
         cgccacgcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAgaga                                                                                                                              >  1:981269/1‑140 (MQ=255)
          gccacgcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCTTTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTTCCCGCCGGTACTGAAGACTGGCGATAAGGGGCCGCACTGGGCAGAGAc                                                                                                                             >  2:797280/1‑140 (MQ=255)
          gccacgcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGAc                                                                                                                             >  1:1408247/1‑140 (MQ=255)
               gcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAAc                                                                                                                        >  1:1359939/1‑140 (MQ=255)
                        gggCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCaa                                                                                                               >  1:1453413/1‑140 (MQ=255)
                        gggCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCaa                                                                                                               >  1:1831700/1‑140 (MQ=255)
                        gggCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGAACAGGTGCCGCATTGCGCAGAGGCGGAACTGCAGGCaa                                                                                                               >  1:921055/1‑140 (MQ=255)
                           cGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAgctgtctc                                                                                                            >  1:1464857/1‑133 (MQ=255)
                             aCTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGAGCCGCACGGGGAAGGGACGGAGCTGCAGGCAATGGGc                                                                                                          >  1:563089/1‑140 (MQ=255)
                               tGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGGCGGAACTGCAGGCAATGGGccc                                                                                                        >  1:734003/1‑140 (MQ=255)
                                                    cTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCTGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACg                                                                                   >  2:1129825/1‑140 (MQ=255)
                                                    cTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACg                                                                                   >  1:180516/1‑140 (MQ=255)
                                                         cATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGCCCCTCTTTGTTCGCGTGAAAAACGGATGc                                                                              >  1:595486/1‑140 (MQ=255)
                                                                   gTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCGCGCTCTGCTCGAGTAAAAAACAGATGCTGGGGCTGAt                                                                    >  1:598554/1‑140 (MQ=255)
                                                                   gTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGAt                                                                    >  2:1437073/1‑140 (MQ=255)
                                                                   gTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGAt                                                                    >  2:456423/1‑140 (MQ=255)
                                                                      ctgctgGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGGTCTGTTCGCGTGAAGAACGGAGGCTGGGGCTGATggc                                                                 >  1:519685/1‑139 (MQ=255)
                                                                      ctgctgGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTATATTCGCGTGAAAAACGGCTGCTGGGGCTGATGGt                                                                 >  2:826083/1‑140 (MQ=255)
                                                                         ctgGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAgg                                                              >  1:1026766/1‑140 (MQ=255)
                                                                             ttCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACAGACGCTGGGACTGATGGTAGgcgcg                                                          >  1:867219/1‑140 (MQ=255)
                                                                              tCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGgcgcgc                                                         >  2:376383/1‑140 (MQ=255)
                                                                              ccccTGGTTGGTTTGCGTGCTCCCCCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGgcgcgc                                                         <  2:1589235/139‑1 (MQ=255)
                                                                               cccTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCt                                                        >  1:1573921/1‑140 (MQ=255)
                                                                                 ctggctggCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGTGTGTAAACCGGATGCTGGGGCTGATGGTAGGCGCGCTgg                                                      >  2:637919/1‑140 (MQ=255)
                                                                                   ggctggctTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTg                                                    >  2:1215031/1‑140 (MQ=255)
                                                                                        gctTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAGACGGATGTTGGGGCTGATGGTGGGCGCGCTGGTGCtgtg                                               >  2:729098/1‑140 (MQ=255)
                                                                                                ttttcccccGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcg                                       <  2:1573921/134‑1 (MQ=255)
                                                                                                  tGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     >  2:1869759/1‑140 (MQ=255)
                                                                                                  tGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     >  2:313345/1‑140 (MQ=255)
                                                                                                  tGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     >  1:415622/1‑140 (MQ=255)
                                                                                                  tGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     >  1:1899246/1‑140 (MQ=255)
                                                                                                  tGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     <  1:1215031/140‑1 (MQ=255)
                                                                                                  ccgccccGCCGGTACTGCAGTCGGGCGATCACGGGCCGCGCTGGGCAGCGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggc                                     <  2:921055/136‑1 (MQ=255)
                                                                                                   gTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggcg                                    <  1:1129825/140‑1 (MQ=255)
                                                                                                   gTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAAAGGATGCTGGGGCGGATCGTAGGCGCGCTGGTGCTGTGGATTTTcggcg                                    >  2:700451/1‑140 (MQ=255)
                                                                                                   ctccccGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggcg                                    <  2:472771/137‑1 (MQ=255)
                                                                                                    tACCCGCCAGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggcgg                                   >  2:1543288/1‑140 (MQ=255)
                                                                                                    cccccGCCGGTACTGATTTCTGGCGATCAGGGGCCTCACGGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggcgg                                   <  2:867219/138‑1 (MQ=255)
                                                                                                    cccccGCCGGTACTGAAGTCTGCCGATCAGGAGCCTCACGGGGCAGAGCCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTcggcgg                                   <  2:598554/138‑1 (MQ=255)
                                                                                                           cgGTACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATtat                            >  2:1413890/1‑140 (MQ=255)
                                                                                                              tACTGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATc                         >  1:1726766/1‑140 (MQ=255)
                                                                                                              tACTGAAGTCTGGCGATCAGGTGCCACACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATc                         >  1:1372790/1‑140 (MQ=255)
                                                                                                                cTGAAGTCTGGCGATTAGGTGCCGCACTGCGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGa                       <  1:663603/140‑1 (MQ=255)
                                                                                                                 tGAAGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGAt                      <  2:1359939/140‑1 (MQ=255)
                                                                                                                   aaGTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGc                    >  2:324477/1‑140 (MQ=255)
                                                                                                                     gTCTGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCcg                  <  1:313345/140‑1 (MQ=255)
                                                                                                                        tGGCGATCAGGTGCCTCACTGGGCACAGATGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               <  1:811913/140‑1 (MQ=255)
                                                                                                                        tGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               <  1:1869759/140‑1 (MQ=255)
                                                                                                                        tGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               >  2:1806818/1‑140 (MQ=255)
                                                                                                                        tGGCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTACTGTGGAATTTAGGGGGTGATGATATCGATGCCGCGa               >  1:523646/1‑140 (MQ=255)
                                                                                                                        gggCGATCAGGTGCCGCTCTGGTCAGAGACGTAACCGCACGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               <  2:503714/139‑1 (MQ=255)
                                                                                                                          gCGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg             <  2:180516/140‑1 (MQ=255)
                                                                                                                           cGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               >  2:1364070/1‑137 (MQ=255)
                                                                                                                           cGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa               <  1:1364070/137‑1 (MQ=255)
                                                                                                                           cGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg             <  1:1412193/139‑1 (MQ=255)
                                                                                                                           cGATCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg             >  2:1412193/1‑139 (MQ=255)
                                                                                                                            gATCAGGTGCCGCACAGGGCAGTGAGGGAACTGCAGGTAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGt           <  1:700451/140‑1 (MQ=255)
                                                                                                                             aTCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGTTGTTTATATGGATGCCGCGATGGTc          >  1:842892/1‑140 (MQ=255)
                                                                                                                             aTCAGGTGCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTc          >  1:1855739/1‑140 (MQ=255)
                                                                                                                                    gCCGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTTGGCGGTGGTTAAATGGATGCCGCGATGGTCGGTTAcc   >  1:503714/1‑139 (MQ=255)
                                                                                                                                     ccGCACTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAg  <  2:415622/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAG  >  NC_000913/3208695‑3208967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑