BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,819,902	T→C	K423K (AAA→AAG)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,819,902	0	T	C	100.0%	428.9 / NA	117	K423K (AAA→AAG)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (56/61); total (56/61)

Only 100 of 120 total aligned reads displayed.

TTACTTTTCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGTTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCA  >  NC_000913/3819765‑3820037

ttacttttCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGctt                                                                                                                                       >  1:1243252/1‑140 (MQ=255)

ttacttttCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGctt                                                                                                                                       >  2:1150556/1‑140 (MQ=255)

     tttCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGccc                                                                                                                                  <  2:1512541/140‑1 (MQ=255)

      ttCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGcccc                                                                                                                                 <  2:417023/140‑1 (MQ=255)

        cgcgATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCcca                                                                                                                               >  1:257668/1‑140 (MQ=255)

          cgATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATATAAGCCAGGTAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGTCATCCAGCCCAAGAACCTGCTTTGCCCccagc                                                                                                                             <  2:657790/140‑1 (MQ=255)

          cgATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCccagc                                                                                                                             <  1:1887636/140‑1 (MQ=255)

               cccGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCCGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGAc                                                                                                                        <  2:1237721/140‑1 (MQ=255)

                  ggCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCGATCGAGCTCAAGAACCGGCTTGGCCCCCAGCCAGACTaa                                                                                                                     >  2:539575/1‑140 (MQ=255)

                    cGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGc                                                                                                                   >  2:1278794/1‑140 (MQ=255)

                      tgtTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGt                                                                                                                 <  1:103863/140‑1 (MQ=255)

                      tgtTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGt                                                                                                                 <  2:127849/140‑1 (MQ=255)

                        ttCTGTAATTGCTCTGGCGTTGATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGa                                                                                                               >  2:1876949/1‑140 (MQ=255)

                        ttCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGa                                                                                                               >  2:117175/1‑140 (MQ=255)

                        ttCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGa                                                                                                               >  1:760099/1‑140 (MQ=255)

                         tCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGaa                                                                                                              >  1:453217/1‑140 (MQ=255)

                             tAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGa                                                                                                          >  1:1106782/1‑140 (MQ=255)

                              aaTTGCTCTGGCGTTCATAAAACCCTAGATAAGATATTTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:555408/140‑1 (MQ=255)

                              aaTTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:1026632/140‑1 (MQ=255)

                              aaTTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:1278794/140‑1 (MQ=255)

                              aaTTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  2:257668/140‑1 (MQ=255)

                              aaTTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  2:1101330/140‑1 (MQ=255)

                              aaTTGCTCTGGCGTTAATAAAAACCGGGATAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:539575/140‑1 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAGAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGt                                                                                                     >  1:224457/1‑140 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:329301/136‑1 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         >  2:329301/1‑136 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGt                                                                                                     >  2:1320365/1‑140 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTACTTTGCCACCAGCCAGACTAACCGTGAAATGAACTGt                                                                                                     >  1:697240/1‑140 (MQ=255)

                                  gCTCTGGCGTTAATAAAAGCCAAGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACGTGCGTTGGCCCCAGCCGGACTCAGCGTGAAATGAACTGt                                                                                                     >  2:555408/1‑140 (MQ=255)

                                   ctctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGtt                                                                                                    >  1:1819106/1‑140 (MQ=255)

                                   ctctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGtt                                                                                                    >  1:1774588/1‑140 (MQ=255)

                                   ctctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGtt                                                                                                    >  2:1204749/1‑140 (MQ=255)

                                    tctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTc                                                                                                   <  1:934648/140‑1 (MQ=255)

                                     ctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  >  1:1447952/1‑140 (MQ=255)

                                     ctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  >  1:1295224/1‑140 (MQ=255)

                                     ctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  >  2:1731144/1‑140 (MQ=255)

                                     ctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  <  2:1774588/140‑1 (MQ=255)

                                     ctGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  >  2:76095/1‑140 (MQ=255)

                                       ggCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGaa                                                                                                         <  1:1140241/131‑1 (MQ=255)

                                       ggCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTg                                                                                                >  1:1898426/1‑140 (MQ=255)

                                       ggCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAACAACCTGCTGTGCACCCAGCCAGACTTAGCGTGAAATGAACTGTTCCTg                                                                                                >  2:599431/1‑140 (MQ=255)

                                        gCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGa                                                                                               <  2:1106782/140‑1 (MQ=255)

                                           ttAATAAATGCCAGGAATAGATATGTTCAAAGCGAGGAGTCTGATGCAACGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACaa                                                                                            <  2:882738/140‑1 (MQ=255)

                                           ttAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  <  2:1909373/134‑1 (MQ=255)

                                           ttAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTcc                                                                                                  >  1:1909373/1‑134 (MQ=255)

                                             aataaAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCGGCCAGACAAAGTGTTAAATGATCTGTTCCTGACAAAc                                                                                          >  1:574911/1‑140 (MQ=255)

                                               taaAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACAt                                                                                        <  2:1447952/140‑1 (MQ=255)

                                                aaaaGCCAGGAAAAGATATGTTAAACGCGTTGAGTCTAATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATc                                                                                       <  1:599431/140‑1 (MQ=255)

                                                   aGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGGTGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGa                                                                                    <  2:760099/140‑1 (MQ=255)

                                                   aGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGa                                                                                    <  2:1603368/140‑1 (MQ=255)

                                                   aGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGa                                                                                    <  1:957189/140‑1 (MQ=255)

                                                   aGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGa                                                                                    <  2:1365354/140‑1 (MQ=255)

                                                     ccAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAg                                                                                  <  1:1516222/140‑1 (MQ=255)

                                                      cAGGAAAAGATATGTTCAAAGCGATGAGTTTGATGCACCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGc                                                                                 <  1:674061/140‑1 (MQ=255)

                                                       aGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCa                                                                                <  1:1320365/140‑1 (MQ=255)

                                                        ggAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCaa                                                                               >  2:298330/1‑140 (MQ=255)

                                                        ggAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCaa                                                                               >  2:1445064/1‑140 (MQ=255)

                                                         gAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCaaa                                                                              >  1:1899710/1‑140 (MQ=255)

                                                              gATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGc                                                                         <  1:298330/140‑1 (MQ=255)

                                                              gATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGc                                                                         <  2:1802222/140‑1 (MQ=255)

                                                              gATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGc                                                                         <  2:1295224/140‑1 (MQ=255)

                                                              gATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGc                                                                         <  1:1445064/140‑1 (MQ=255)

                                                                tatGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAg                                                                       <  1:1113352/140‑1 (MQ=255)

                                                                   gTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAATCGTGAAATCAACAGTTCCTGACAAACATCACAAGCAAAGTAGCAGGTc                                                                    >  2:733606/1‑140 (MQ=255)

                                                                   gTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGATGCAAAGAAGCAGGTc                                                                    >  2:1391150/1‑140 (MQ=255)

                                                                   gTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTc                                                                    >  2:21202/1‑140 (MQ=255)

                                                                        aaGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGa                                                               <  2:1883251/140‑1 (MQ=255)

                                                                            gATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTa                                                           <  1:1477387/140‑1 (MQ=255)

                                                                               gAGTCTGATGCAGCGTGCGCCACCCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAg                                                        <  1:673963/140‑1 (MQ=255)

                                                                                    tGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGtt                                                   >  2:1535197/1‑140 (MQ=255)

                                                                                      aTGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGtttt                                                 <  2:235479/140‑1 (MQ=255)

                                                                                          aGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTgg                                             <  2:1898426/140‑1 (MQ=255)

                                                                                           gcgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGc                                            >  2:433979/1‑140 (MQ=255)

                                                                                            tgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCg                                           >  2:292501/2‑140 (MQ=255)

                                                                                            cgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGt                                                             >  1:319963/1‑122 (MQ=255)

                                                                                            cgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTACAGCGGTTTTCTGGCg                                           >  1:829934/1‑140 (MQ=255)

                                                                                            cgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCg                                           >  1:323660/1‑140 (MQ=255)

                                                                                            cgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCg                                           >  2:1753516/1‑140 (MQ=255)

                                                                                            cgcgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCg                                           >  1:170663/1‑140 (MQ=255)

                                                                                              cgcgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGgt                                         >  2:148634/1‑140 (MQ=255)

                                                                                                cgcCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGgtgt                                       >  2:1365595/1‑140 (MQ=255)

                                                                                                    aaCCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGt                                   <  1:1387399/140‑1 (MQ=255)

                                                                                                       cGGCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCACAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGtttt                                <  2:1174384/140‑1 (MQ=255)

                                                                                                         gCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGt                              <  2:1068360/140‑1 (MQ=255)

                                                                                                         gCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGt                              <  1:1731144/140‑1 (MQ=255)

                                                                                                         gCCTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGt                              <  2:323660/140‑1 (MQ=255)

                                                                                                           cTCACCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTAc                            >  2:1316741/1‑140 (MQ=255)

                                                                                                              aCCAATGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGtt                         >  1:1426305/1‑140 (MQ=255)

                                                                                                                   tGCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCG‑GAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCAc                   >  2:1608876/1‑140 (MQ=255)

                                                                                                                    gCCATCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCAc                   <  1:1535197/140‑1 (MQ=255)

                                                                                                                        tCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACcgcg               >  2:322809/1‑140 (MQ=255)

                                                                                                                        tCCAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACcgcg               <  1:1267485/140‑1 (MQ=255)

                                                                                                                         ccAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCgcgc              <  2:348779/140‑1 (MQ=255)

                                                                                                                         ccAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCgcgc              <  1:1564314/140‑1 (MQ=255)

                                                                                                                         ccAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCgcgc              <  2:1668858/140‑1 (MQ=255)

                                                                                                                         ccAGCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCgcgc              <  2:1899710/140‑1 (MQ=255)

                                                                                                                            gCCCAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGccac           <  1:1753516/140‑1 (MQ=255)

                                                                                                                              ccAAGAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGccacac         <  1:1668639/140‑1 (MQ=255)

                                                                                                                                  gAACCTGCTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGccacaccaca     >  1:1583218/1‑140 (MQ=255)

                                                                                                                                     ccTGCTTTCCCCCCAGGCAGACCAAGCGTGAGATGGACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCAcatca  <  2:845728/140‑1 (MQ=255)

TTACTTTTCGCGATCCCCGGCGTGTTCTGTAATTGCTCTGGCGTTAATAAAAGCCAGGAAAAGATATGTTCAAAGCGATGAGTCTGATGCAGCGCGCGCCAACCGGCCTCACCAATGCCATCCAGCCCAAGAACCTGTTTTGCCCCCAGCCAGACTAAGCGTGAAATGAACTGTTCCTGACAAACATCAGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCA  >  NC_000913/3819765‑3820037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`