Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,988,542	C→T	P175P (CCG→CCA)	hemX ←	PF04375 family protein HemX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,988,542	0	C	T	100.0%	318.9 / NA	93	P175P (CCG→CCA)	hemX	PF04375 family protein HemX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/42);  total (51/42)

TATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAG  >  NC_000913/3988406‑3988678                                                                                                                                         |                                                                                                                                         tATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTggg                                                                                                                                       <  1:223167/140‑1 (MQ=255) tATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTggg                                                                                                                                       <  1:1672078/140‑1 (MQ=255) tATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTggg                                                                                                                                       <  1:1565845/140‑1 (MQ=255) tATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTggg                                                                                                                                       <  2:59794/140‑1 (MQ=255)       ccAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGtcattc                                                                                                                                 >  1:1558379/1‑140 (MQ=255)       ccAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGt                                                                                                                                      >  1:355423/1‑135 (MQ=255)       ccAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGt                                                                                                                                      <  2:355423/135‑1 (MQ=255)         aGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGGAATCGCCCCACGAACGTTAATACGACTTGGTtcattcat                                                                                                                               >  2:864142/1‑140 (MQ=255)              cAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCg                                                                                                                          <  2:1002020/140‑1 (MQ=255)                    aTCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCTAGGc                                                                                                                    >  2:1208160/1‑140 (MQ=255)                     tCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCt                                                                                                                   <  1:274988/140‑1 (MQ=255)                     tCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCt                                                                                                                   >  2:274988/1‑140 (MQ=255)                     tCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAAATGCCCGACAAACCGGAGGCAGACTTCGGTCGTTCATATCCGCCAGGCt                                                                                                                   >  2:922432/1‑140 (MQ=255)                      cTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTg                                                                                                                  >  1:452054/1‑140 (MQ=255)                      cTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTg                                                                                                                  >  1:1115966/1‑140 (MQ=255)                        aCCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGc                                                                                                                <  2:1248926/140‑1 (MQ=255)                         ccTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCg                                                                                                               >  1:387498/1‑140 (MQ=255)                          cTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCTAGGCTGGCGt                                                                                                              <  1:1208160/140‑1 (MQ=255)                           tGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCt                                                                                                                   >  2:1021751/1‑134 (MQ=255)                           tGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCt                                                                                                                   <  1:1021751/134‑1 (MQ=255)                            gATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCt                                                                                                            >  2:1161691/1‑140 (MQ=255)                             aTTTGAAAGCTGATTCAGCTTATGGCTGAAGCCGTCAGAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTg                                                                                                           <  1:634376/140‑1 (MQ=255)                             aTTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTg                                                                                                           <  2:387498/140‑1 (MQ=255)                                   aaGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGAATTGGGTCATTCATATCAGCCATGCTGGCGTCTGCACttt                                                                                                     >  2:617283/1‑140 (MQ=255)                                    aGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGAAATCAGACCTGGGTCAGTCATATCCACCAGTCTGGCGTCTGCACtttt                                                                                                    >  2:713056/1‑140 (MQ=255)                                       tGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAg                                                                                                 <  1:1598633/140‑1 (MQ=255)                                        gATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGc                                                                                                <  1:1392389/140‑1 (MQ=255)                                         aTTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCa                                                                                               >  1:1860614/1‑140 (MQ=255)                                                  aaGGATGATGCCGTCTTAATCCCCCAGCGATACTGCAAAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAg                                                                                      <  1:617283/140‑1 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAg                                                                                                 <  2:1373378/128‑1 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAg                                                                                                 >  1:1373378/1‑128 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGc                                                                                     >  2:391550/1‑140 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGc                                                                                     >  1:455819/1‑140 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGc                                                                                     >  2:640107/1‑140 (MQ=255)                                                   aGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGc                                                                                     >  2:352427/1‑140 (MQ=255)                                                       tgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTc                                                                                 >  1:1571873/1‑140 (MQ=255)                                                       tgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTc                                                                                 >  1:1202200/1‑140 (MQ=255)                                                         atgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGGTGGCGTCGGCAATTATCAGCAGCGCTGCAGCGGTCGt                                                                               >  1:902420/1‑140 (MQ=255)                                                         atgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               >  1:1313702/1‑140 (MQ=255)                                                         atgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               <  2:1115966/140‑1 (MQ=255)                                                         atgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               <  2:1860614/140‑1 (MQ=255)                                                         agacagTCATAATCCGCCTGCGCTACTGCAGAAAGGCAGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               <  1:833028/135‑1 (MQ=255)                                                          tgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCCGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              >  1:1694201/1‑140 (MQ=255)                                                          tgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              >  1:961505/1‑140 (MQ=255)                                                          tgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              <  2:452054/140‑1 (MQ=255)                                                          tgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              <  1:352427/140‑1 (MQ=255)                                                               tCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTc                                                                         <  2:1202200/140‑1 (MQ=255)                                                               tCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGGCTGCACTTTTCAGCTACGCTGCAGCGGTCGTGACGTc                                                                         >  1:834404/1‑140 (MQ=255)                                                                cATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               >  1:649077/1‑133 (MQ=255)                                                                cATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGt                                                                               <  2:649077/133‑1 (MQ=255)                                                                 aTAATCCGCCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACGTGGGTCATTCATATCCGCCAGGCTGGAGTCTGCCCTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCt                                                                       <  2:1326461/140‑1 (MQ=255)                                                                    aTCCACCTGCGATACTTCAGAAAGGCTGGCGGTTTCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGAt                                                                    <  2:834404/140‑1 (MQ=255)                                                                    aTCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGAt                                                                    <  2:455819/140‑1 (MQ=255)                                                                    aTCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGAt                                                                    <  2:1571873/140‑1 (MQ=255)                                                                      ccACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCg                                                                  <  2:1558379/140‑1 (MQ=255)                                                                         ccTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                               >  1:1187196/1‑140 (MQ=255)                                                                          cTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAAGGCTGCAGCAGTCGCCACGTCCTAATctgtct                                                              >  2:833028/1‑135 (MQ=255)                                                                          cTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              <  2:39391/124‑1 (MQ=255)                                                                          cTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTg                                                                              >  1:39391/1‑124 (MQ=255)                                                                          cTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTcc                                                              >  2:1366488/1‑140 (MQ=255)                                                                          cTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGGCTGCACTTGTCAACAATGCTGCAGCAGTCGTGACGTCCTGATCGCTcc                                                              >  2:499183/1‑140 (MQ=255)                                                                           tGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCca                                                             >  2:1109371/1‑140 (MQ=255)                                                                           tGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCca                                                             >  1:69158/1‑140 (MQ=255)                                                                             cGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCcaca                                                           >  2:65163/1‑140 (MQ=255)                                                                               aTACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGc                                                         >  2:1213289/1‑140 (MQ=255)                                                                                   tGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTATTCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTtcc                                                     >  2:1318251/1‑140 (MQ=255)                                                                                    gCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTtccg                                                    >  1:1881486/1‑140 (MQ=255)                                                                                     cAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTtccgt                                                   >  1:1852352/1‑140 (MQ=255)                                                                                         aaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCgg                                               <  1:1109371/140‑1 (MQ=255)                                                                                         aaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCgg                                               <  1:640107/140‑1 (MQ=255)                                                                                          aGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                               >  1:114314/1‑123 (MQ=255)                                                                                          aGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                               <  2:114314/123‑1 (MQ=255)                                                                                          aGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGc                                              >  2:254680/1‑140 (MQ=255)                                                                                             cTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAg                                           >  1:1672633/1‑140 (MQ=255)                                                                                             cTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAg                                           >  1:1887646/1‑140 (MQ=255)                                                                                              tGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGt                                          <  2:69158/140‑1 (MQ=255)                                                                                               ggCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGAGGTCATGATCGCTGCACACCTTCCGTCCGGCGAGtt                                         >  2:608732/1‑140 (MQ=255)                                                                                               ggCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGtt                                         >  2:1517867/1‑140 (MQ=255)                                                                                               ggCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGtt                                         >  1:1909662/1‑140 (MQ=255)                                                                                               ggCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTCCAGCGGTCATGAAGACGAGATCGCTACACAGATTCCGTCAGGCGAGtt                                         >  2:916557/1‑140 (MQ=255)                                                                                                     atcatcGGTAATTGCACGACGAACGCTAACCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCGCTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCa                                   <  1:713056/140‑1 (MQ=255)                                                                                                      tcatcGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAg                                  >  1:134165/1‑140 (MQ=255)                                                                                                          cGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGaaaa                              >  2:1073013/1‑140 (MQ=255)                                                                                                          cGGTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGaaaa                              >  1:1177009/1‑140 (MQ=255)                                                                                                           ggTAATTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAAt                             <  2:1412061/140‑1 (MQ=255)                                                                                                               aTTGCCCGACGAACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGc                         <  2:1672633/140‑1 (MQ=255)                                                                                                                ttGCCCGACGAACGGTACTCAGACTTGGGTCCTTCTTATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGcc                        <  2:704991/140‑1 (MQ=255)                                                                                                                 tGCCCGACGAACGGTCATCAGACTTAGGTCATTCATAGCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCt                       <  1:864142/140‑1 (MQ=255)                                                                                                                       acgaACGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCca                 >  1:1820357/1‑140 (MQ=255)                                                                                                                            cGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCagcagc            <  1:65163/140‑1 (MQ=255)                                                                                                                            cGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCagcagc            <  1:1517867/140‑1 (MQ=255)                                                                                                                            cGGTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCagcagc            <  2:1887646/140‑1 (MQ=255)                                                                                                                            cGGTAATCAGACTTGGGACATTAATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCagcagc            <  2:902420/140‑1 (MQ=255)                                                                                                                            aggTAATCAGACTTGGGCCATTTATATCCGCAAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCagcagc            <  2:508011/139‑1 (MQ=255)                                                                                                                              gTAATCAGACTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCa          <  2:961505/140‑1 (MQ=255)                                                                                                                                      aCTTGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAg  >  1:281377/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAG  >  NC_000913/3988406‑3988678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑