Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,779	T→C	A142A (GCA→GCG)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,779	0	T	C	100.0%	363.6 / NA	98	A142A (GCA→GCG)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/48);  total (50/48)

CGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGG  >  NC_000913/4193643‑4193915                                                                                                                                         |                                                                                                                                         cgcTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCa                                                                                                                                       <  1:1821650/140‑1 (MQ=255)  gcTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCa                                                                                                                                       >  1:349605/1‑139 (MQ=255)  gcTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCa                                                                                                                                       <  2:349605/139‑1 (MQ=255)   cTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGa                                                                                                                                     >  2:1715946/1‑140 (MQ=255)   cTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGa                                                                                                                                     >  1:1429030/1‑140 (MQ=255)   cTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGa                                                                                                                                     >  2:35903/1‑140 (MQ=255)      acgacgGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAgg                                                                                                                                  <  2:1874681/140‑1 (MQ=255)      acgacgGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAgg                                                                                                                                  <  1:1301674/140‑1 (MQ=255)        gacgGCGATCCTGCCGACGCCCTTGGCTATCACCGCAGGCGCGGGGGCCGGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCa                                                                                                                                <  2:1891337/140‑1 (MQ=255)        gacgGCGATACTGTCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCa                                                                                                                                <  2:1473401/140‑1 (MQ=255)        gacgGCGATACTGCCGACACCCGTTGCTATCCCCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCa                                                                                                                                <  1:1715946/140‑1 (MQ=255)          cgGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATc                                                                                                                              <  1:1169823/140‑1 (MQ=255)                         cccccGTTGCTATCACCTCAGGCGCGCGTGCTAGATGGAAACCGCCAAGCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTc                                                                                                               <  2:701305/138‑1 (MQ=255)                         cccccGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTc                                                                                                               <  1:1200042/138‑1 (MQ=255)                          acCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCg                                                                                                              >  2:1745608/1‑140 (MQ=255)                          acCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCg                                                                                                              >  2:246335/1‑140 (MQ=255)                            cggTTGCTATCCCCGCAGCGGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCggg                                                                                                            <  2:1744634/138‑1 (MQ=255)                             cGTTGCTATCACCGCAGGCGCGTGTACCAGACTGATATCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGa                                                                                                           <  1:739090/140‑1 (MQ=255)                             cGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGa                                                                                                           <  2:1215889/140‑1 (MQ=255)                               ttGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAac                                                                                                         >  1:154693/1‑140 (MQ=255)                               ttGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAac                                                                                                         >  2:1405446/1‑140 (MQ=255)                                        ccGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTccc                                                                                                >  1:1489288/1‑140 (MQ=255)                                          gCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAg                                                                                              <  2:1351839/140‑1 (MQ=255)                                           cAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgc                                                                                             <  1:420117/140‑1 (MQ=255)                                            aGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGTTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            <  1:663893/140‑1 (MQ=255)                                            aGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            <  1:1405446/140‑1 (MQ=255)                                            aGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            >  2:1357981/1‑140 (MQ=255)                                            aGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            <  1:137617/140‑1 (MQ=255)                                            aGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            <  1:56188/140‑1 (MQ=255)                                             ggCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcgc                                                                                           >  1:280917/1‑140 (MQ=255)                                              gcgcgcGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGcgcg                                                                                          >  1:478175/1‑140 (MQ=255)                                               cgcgcgTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGa                                                                                         >  2:1287163/1‑140 (MQ=255)                                                    gTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATaa                                                                                    <  1:35903/140‑1 (MQ=255)                                                      gCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAaga                                                                                  >  2:1388174/1‑140 (MQ=255)                                                         aGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            <  2:1304858/127‑1 (MQ=255)                                                         aGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAgcg                                                                                            >  1:1304858/1‑127 (MQ=255)                                                              gATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGtg                                                                          >  2:475979/1‑140 (MQ=255)                                                              aaTACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGtg                                                                          <  1:1388174/139‑1 (MQ=255)                                                               aTACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGtgc                                                                         <  1:435143/140‑1 (MQ=255)                                                                tACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGtgct                                                                        >  1:1413970/1‑140 (MQ=255)                                                                    gCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGccc                                                                    >  2:1478101/1‑139 (MQ=255)                                                                    gCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTAGCGTCGGGTACAAATGACCCAGCGCGATATGAGAGGGGCGTGCTGCCa                                                                    >  2:577266/1‑140 (MQ=255)                                                                     ccAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAg                                                                   >  1:1052671/1‑140 (MQ=255)                                                                       aaTCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAgcg                                                                 <  1:1287163/140‑1 (MQ=255)                                                                       aaTCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAgcg                                                                 <  2:318139/140‑1 (MQ=255)                                                                         tCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGcgcg                                                               <  2:1489288/140‑1 (MQ=255)                                                                         tCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGcgcg                                                               >  1:1229365/1‑140 (MQ=255)                                                                            ccACGGTGGGATAATTCGCCAGTCGCTCATCATGCCGCGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACg                                                            <  1:486536/140‑1 (MQ=255)                                                                            ccACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACg                                                            <  2:1193127/140‑1 (MQ=255)                                                                             cACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGt                                                           >  2:983925/1‑140 (MQ=255)                                                                                ggTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGa                                                        >  2:387489/1‑140 (MQ=255)                                                                                ggTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGa                                                        <  1:462744/140‑1 (MQ=255)                                                                                 gTGGGATAATCCGCCCGTCGCTTAACATGCCGTGCCACCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       <  1:577266/140‑1 (MQ=255)                                                                                 gTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       <  2:280917/140‑1 (MQ=255)                                                                                   gggATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAttt                                                     <  1:475979/140‑1 (MQ=255)                                                                                    ggATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       <  2:969167/137‑1 (MQ=255)                                                                                    ggATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       >  1:969167/1‑137 (MQ=255)                                                                                     gATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTcc                                                   <  1:1357981/140‑1 (MQ=255)                                                                                     gATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTcc                                                   >  2:186474/1‑140 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       <  2:113821/131‑1 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       >  1:113821/1‑131 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCata                                                <  2:1199796/138‑1 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCata                                                >  1:1199796/1‑138 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATc                                              >  2:1296465/1‑140 (MQ=255)                                                                                          tCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATc                                              >  2:1392467/1‑140 (MQ=255)                                                                                            cGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGt                                            >  2:1023990/1‑140 (MQ=255)                                                                                              ccAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCa                                          >  2:1800242/1‑140 (MQ=255)                                                                                              ccAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCa                                          >  1:1231276/1‑140 (MQ=255)                                                                                                  tCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       <  1:1395436/123‑1 (MQ=255)                                                                                                  tCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGAt                                                       >  2:1395436/1‑123 (MQ=255)                                                                                                  tCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGAGCTGCCAGCGCAACATCGATTACCATATCGTCAtgtg                                      >  1:623280/1‑140 (MQ=255)                                                                                                  tCGCTCAACATGCCGAGCCAGCCGTTCCAGCCCCTGCCGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCAtgtg                                      <  1:487948/140‑1 (MQ=255)                                                                                                     cTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTc                                           <  1:784616/132‑1 (MQ=255)                                                                                                     cTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCg                                   >  1:1433581/1‑140 (MQ=255)                                                                                                     cTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCCATTTCCATATCGTc                                           >  2:784616/1‑132 (MQ=255)                                                                                                     cTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCACGACGTCGATTTAAATATCGTAATGTGTCg                                   >  2:884958/1‑140 (MQ=255)                                                                                                            tGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGcc                            <  1:1745608/140‑1 (MQ=255)                                                                                                            tGCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCATGCTGCCAACGCGACGGCGATTTCCGTATCATCATGTGTCGAAACGcc                            >  2:487948/1‑140 (MQ=255)                                                                                                             gCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGTGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGccc                           >  1:1125292/1‑140 (MQ=255)                                                                                                             gCCGTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGccc                           >  2:1828099/1‑140 (MQ=255)                                                                                                                gTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGc                        >  2:416754/1‑140 (MQ=255)                                                                                                                gTGCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGc                        >  2:1570811/1‑140 (MQ=255)                                                                                                                  gCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGGCAGCGCGAGGTCAATTGCCATATCGCCATGGGTCGAAACGCCCAGCCg                      >  2:772168/1‑140 (MQ=255)                                                                                                                  gCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGccc                           <  1:1397980/135‑1 (MQ=255)                                                                                                                  gCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGccc                           >  2:1397980/1‑135 (MQ=255)                                                                                                                  gCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCg                      <  1:1717762/140‑1 (MQ=255)                                                                                                                  gCCAGCTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCg                      >  1:202449/1‑140 (MQ=255)                                                                                                                       cTGTTCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGc                 >  1:195450/1‑140 (MQ=255)                                                                                                                          ttCCAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTg              <  2:478175/140‑1 (MQ=255)                                                                                                                            ccAGCCCCTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGcc            <  1:1392467/140‑1 (MQ=255)                                                                                                                                   cTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGa     <  2:202449/140‑1 (MQ=255)                                                                                                                                   cTGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGa     <  2:1231276/140‑1 (MQ=255)                                                                                                                                    tGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAt    <  1:186474/140‑1 (MQ=255)                                                                                                                                    tGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAt    >  1:1266567/1‑140 (MQ=255)                                                                                                                                    tGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAt    >  2:1499550/1‑140 (MQ=255)                                                                                                                                    tGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAt    >  1:1370041/1‑140 (MQ=255)                                                                                                                                    tGCGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAt    >  2:1837845/1‑140 (MQ=255)                                                                                                                                      cGGCGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATgg  <  1:1800242/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGG  >  NC_000913/4193643‑4193915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑