Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,291,056	C→T	A194V (GCG→GTG)	nrfD →	putative menaquinol‑cytochrome c reductase subunit NrfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,291,056	0	C	T	100.0%	234.2 / NA	69	A194V (GCG→GTG)	nrfD	putative menaquinol‑cytochrome c reductase subunit NrfD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/46);  total (23/46)

GATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGT  >  NC_000913/4290928‑4291189                                                                                                                                 |                                                                                                                                       gATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGGCGGTGCTGTTCCGCCTTTCCGGCATATCGTATGGGGtggcggctgtct                                                                                                                             >  2:856768/1‑134 (MQ=255) gATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCG‑‑GCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTg                                                                                                                           <  1:23967/140‑1 (MQ=255)    gctggtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGa                                                                                                                          <  2:1118734/140‑1 (MQ=255)     ctggtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGAt                                                                                                                         <  1:1153279/140‑1 (MQ=255)     ctggtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGAt                                                                                                                         <  2:1738030/140‑1 (MQ=255)      tggtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATc                                                                                                                        <  1:962837/140‑1 (MQ=255)        gtgctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGGGgggcggggcgc                                                                                                                      >  2:1895654/1‑133 (MQ=255)         tgctggCGGTGTTGTTGGGGGCTTATGCCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGcc                                                                                                                     <  1:393461/140‑1 (MQ=255)          gctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCa                                                                                                                    <  2:1708851/140‑1 (MQ=255)           ctggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCAt                                                                                                                   <  1:1895654/140‑1 (MQ=255)           agacaggTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCAt                                                                                                                   <  1:1360956/135‑1 (MQ=255)             ggCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGGGGGGCGGGTCGcccttg                                                                                                                 >  2:1745387/1‑136 (MQ=255)                ggTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGa                                                                                                              <  1:1140263/140‑1 (MQ=255)                ggTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCATTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGa                                                                                                              <  1:75176/140‑1 (MQ=255)                       ttgGGGGCTTATACCGGCTTTCGGCAGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCa                                                                                                       <  1:714580/140‑1 (MQ=255)                            ggCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTAAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTAGCGGTGGTGCTCGTGGCCATGGCGATACGCCAACGCa                                                                                                  >  1:490002/1‑140 (MQ=255)                            ggCTTATACCGGCTTTATGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCCTGGCGGTACGCCAACGcc                                                                                                  >  2:1251460/1‑139 (MQ=255)                              cTTATACCGGCTTTCTGTTGTCGGCGCTGAAATCGAATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGt                                                                                                <  2:699669/140‑1 (MQ=255)                              cTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGt                                                                                                >  1:96796/1‑140 (MQ=255)                                tataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGGTCGCCCTGGCGATACGCCCACGCAGTaa                                                                                              >  2:1334619/1‑140 (MQ=255)                                 ataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCCTGGGGGTACCCCCACGCAGGaaa                                                                                             >  2:1377958/1‑139 (MQ=255)                                 ataCCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAAc                                                                                             <  1:1175516/140‑1 (MQ=255)                                 ataCCGGCTTTCTGCTGTCGGCGCTG‑AATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGGTACGCCCACGCCGTAAcc                                                                                            >  2:1134247/1‑140 (MQ=255)                                 ataCCGGCTTTCTGCTGACGGCCCTGAAATCTTATACGTTCCTAAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAAc                                                                                             <  2:490002/140‑1 (MQ=255)                                   aCCGGCTTTCTGCTGTCGGCGCGGAAATCGTATCCGTTCGTCAATAACTCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAAccc                                                                                           <  2:761197/140‑1 (MQ=255)                                    ccGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCg                                                                                          <  1:1745387/140‑1 (MQ=255)                                    ccGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCg                                                                                          <  2:45/140‑1 (MQ=255)                                    ccGGCTTTCTGCTGTCGCCGCTAAAATCGTACCCGTTCCACAATAACCAGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCg                                                                                          <  2:488125/140‑1 (MQ=255)                                    ccGGCTTTCTGCTCCCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCg                                                                                          <  2:1775950/140‑1 (MQ=255)                                      ggCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCa                                                                                        >  1:1604696/1‑140 (MQ=255)                                       gCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGGGCTGATCGCCATGGCGGTACGCCAACGCAGGAACCCGCAt                                                                                       >  2:174653/1‑140 (MQ=255)                                       gCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCAt                                                                                       >  1:1482736/1‑140 (MQ=255)                                        cTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCAtt                                                                                      >  1:1060159/1‑140 (MQ=255)                                                  tCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgc                                                                            <  1:1251460/140‑1 (MQ=255)                                                   cGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcg                                                                           >  1:249531/1‑140 (MQ=255)                                                   cGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcg                                                                           <  2:1482736/140‑1 (MQ=255)                                                    ggCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcgc                                                                          <  1:76205/140‑1 (MQ=255)                                                    ggCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcgc                                                                          <  1:174653/140‑1 (MQ=255)                                                    ggCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcgc                                                                          <  2:199933/140‑1 (MQ=255)                                                    ggCGCTGAAATCGTATCCGTTCCTCAATAACCCGAACCTTCCGGTGCTATTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAgcgc                                                                          <  2:599487/140‑1 (MQ=255)                                                     gcgcTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCa                                                                         <  2:96796/140‑1 (MQ=255)                                                            aaTCGTATCCGTTCCTCAATGACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGGTCGCCATGGCGATACGCCAACGTAGTAACCCGCATTCCACGGAAGCGCAGTTTGTa                                                                  <  1:1173268/140‑1 (MQ=255)                                                              tCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTaca                                                                <  2:249531/140‑1 (MQ=255)                                                              tCGTATCCGTTCCTCAAGAACCCGATCCTGCTGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTaca                                                                <  2:756393/140‑1 (MQ=255)                                                              tCGTATCCGTTCCTCAACAACCCGATCCTGCCGGTCCTGTTCCTCTTCGCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTaca                                                                <  2:639092/140‑1 (MQ=255)                                                                  aTCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGt                                                            <  1:1334619/140‑1 (MQ=255)                                                                     cGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCCTGGCGGTACGCCCACGCAGTAACCCGCCTTCCACGGGAGCGCAGGTTGTACACCGTCTg                                                         >  2:121523/1‑140 (MQ=255)                                                                          cTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAAt                                                   <  1:1479961/140‑1 (MQ=255)                                                                            agacagcccGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATcc                                                 <  1:1586433/134‑1 (MQ=255)                                                                              aTAACCCGATCCTGCTGGTGCTTTTTCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCt                                               <  1:932058/140‑1 (MQ=255)                                                                              aTAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACGCCGTATG‑GAAATCCCt                                               <  2:1060159/140‑1 (MQ=255)                                                                              aTAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCt                                               <  1:1356491/140‑1 (MQ=255)                                                                                  cccGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCGGGTTGCCCTGGCGGTACGCCCACGCAGGAACCCGCATTCCCCGGAAGCGCCGTTTGTACACCGGATG‑GAAATCCCCGTgg                                           >  2:293893/1‑140 (MQ=255)                                                                                  cccGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGCGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTgg                                           >  2:1103674/1‑140 (MQ=255)                                                                                    cGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTa                                         <  1:1361781/140‑1 (MQ=255)                                                                                     gATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTAt                                        <  1:1215794/140‑1 (MQ=255)                                                                                     gATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTAt                                        <  2:1604696/140‑1 (MQ=255)                                                                                      aTCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTTCGGTGTGGCGGTTGGGCCGATCGCCCTGGCGGTACGCCAAAGCAGGAACCCACAATCCACCGAAGCGCCGTTTTTACACCGGATG‑GGAATCCCCCTGGTATg                                       >  2:1300815/1‑140 (MQ=255)                                                                                      aTCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGGTCGCCCTTGGGGTAACCCCACGCCGGAAACCGCATTTCCCCGGAGCGCCGGTTGTTCACCCTATA‑GAAAAACCCGTggggtg                                       >  2:1852734/1‑136 (MQ=255)                                                                                      aTCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGCGGCGCTTATCCCCATGGCGAAACGCCCACACCAGAACCCCCCTTTCACCGGAGCGCAGGTTGTACACCCTATT‑GGAAGCCCCGTgggaag                                       >  2:743325/1‑136 (MQ=255)                                                                                      aTCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGCGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATg                                       <  1:1103674/140‑1 (MQ=255)                                                                                            ccGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCCCCATGGCGATACGCCCACCCAGGAAACCCCATTCCCTGGAAGCGCTGTTGTTTAACCGTATGAGAAAACCC‑GTGGGATggggggg                                 >  2:489338/1‑137 (MQ=255)                                                                                             cGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGGGCTGGTCGCCCTGGGGGTACGCCCACGCAGGAAACCGCATTCCCCAGCAGCGCAGGTTCTACACCCTATG‑GGAAACTCTGTGGGAAggggggga                                >  2:869434/1‑136 (MQ=255)                                                                                             cGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGaa                                <  2:1214315/140‑1 (MQ=255)                                                                                                     ttCCTCTTCTCCGGCATCTCGTCCGGTGTGGCGGTGGCGCTGGTCGCCATGGCGATACGCCCACGCAGTAAACCCCCTTCCACGGGAGCGCCGTTTTTACACCGTATG‑GAAATCCCCGTGGGATGGGGGGAAAACTTCct                        >  2:351932/1‑140 (MQ=255)                                                                                                            tctcCGGCATCTCGTTCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCACATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCtggtg                 <  1:1300815/140‑1 (MQ=255)                                                                                                                       tcGTCCGGTGTGGCGGTGGCGCTGATCGCCATGTCGATAGGCCAACGCTGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGt      <  2:814899/140‑1 (MQ=255)                                                                                                                       tcGTCCGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGt      <  1:1431436/140‑1 (MQ=255)                                                                                                                          tCCGGTGTGGCGGTGGCGCTGGTCGCCATGGCGATACGCCAACGCAGTAAACCGCATTCCACCGAAGCGCAGGTTTTTAACCCTATG‑GGAATCCCCTTGGTATGGGGGGAAATCTTCTTCCTGGGGGGGTTTTTTGTCgg   >  2:796119/1‑140 (MQ=255)                                                                                                                           ccGGTGTGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGt  <  1:1134247/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       GATGCTGGTGCTGGCGGTGTTGTTGGGGGCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATCCTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG‑GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGT  >  NC_000913/4290928‑4291189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑