Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,365,583	A→G	G397G (GGT→GGC)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,365,583	0	A	G	100.0%	256.0 / NA	73	G397G (GGT→GGC)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/38);  total (35/38)

GGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGC  >  NC_000913/4365447‑4365717                                                                                                                                           |                                                                                                                                       ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGccc                                                                                                                                     <  2:407429/140‑1 (MQ=255) ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGccc                                                                                                                                     >  2:40923/1‑140 (MQ=255) ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACAAATTTACCGATACGGGTAGTGccc                                                                                                                                     >  2:1899310/1‑140 (MQ=255)    gTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCgtg                                                                                                                                  >  2:1415084/1‑140 (MQ=255)       aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTca                                                                                                                               <  1:1863943/140‑1 (MQ=255)       aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTca                                                                                                                               <  1:1914310/140‑1 (MQ=255)          gCCCCGCGATTGATCGTTACAGCATCAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTcatcc                                                                                                                            <  1:788803/140‑1 (MQ=255)          gCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTcatcc                                                                                                                            <  2:1487891/140‑1 (MQ=255)            cccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTcatccat                                                                                                                          <  2:1542124/140‑1 (MQ=255)            cccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTcatccat                                                                                                                          <  2:246616/140‑1 (MQ=255)             ccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTcatccatc                                                                                                                         >  1:1007617/1‑140 (MQ=255)               gcgATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATAGTTGAAGAGGAAGTTAGCGATACGGCTAGTGCCCGTGTCATCCATCTg                                                                                                                       >  2:536727/1‑140 (MQ=255)                      aTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACAAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCgca                                                                                                                <  1:1899310/140‑1 (MQ=255)                          ttACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCaa                                                                                                            >  1:1585621/1‑140 (MQ=255)                           tACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAAc                                                                                                           <  1:1729899/140‑1 (MQ=255)                           tACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAAc                                                                                                           <  1:40923/140‑1 (MQ=255)                            aCAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAAcc                                                                                                          >  1:1760291/1‑140 (MQ=255)                              aGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAg                                                                                                        <  1:1415084/140‑1 (MQ=255)                                cATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCg                                                                                                      >  1:1822363/1‑140 (MQ=255)                                   gAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCg                                                                                                   <  2:1007617/140‑1 (MQ=255)                                     aGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCg                                                                                                   >  2:1031582/1‑140 (MQ=255)                                     aGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCggg                                                                                                 <  2:1822363/140‑1 (MQ=255)                                     aGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCAGCTGTACCGCTGCAACCAGCGTCggg                                                                                                 >  1:894618/1‑140 (MQ=255)                                          cccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGAACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGt                                                                                            >  1:1863482/1‑140 (MQ=255)                                            cAGCACGAAGCGGAAGCCAACGTCCAGGGCACCACGC‑‑AGAGTACCCGGTATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCg                                                                                          <  2:537492/140‑1 (MQ=255)                                             aGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCg                                                                                          <  2:119660/139‑1 (MQ=255)                                             aGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCg                                                                                          >  1:119660/1‑139 (MQ=255)                                               cACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGACCGTGTCAGCCAGCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCa                                                                                       >  2:587071/1‑140 (MQ=255)                                                 cgaagccgaagcAAACTCCCAGGGCAACACAA‑‑AAACTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGa                                                                                     <  2:894618/140‑1 (MQ=255)                                                 cgaagccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGa                                                                                     <  1:1641328/140‑1 (MQ=255)                                                 cgaagccgaagcAAAAGGCCAGCGCTACATCC‑‑AGAGTTCCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGa                                                                                     <  1:536727/140‑1 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                  >  2:1878539/1‑140 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                  >  2:188341/1‑140 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                  >  1:1093682/1‑140 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                  >  1:141416/1‑140 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTATCATCCATGTGTGCCGTAGCAACCAACGTCCGGTAGGTCGGCAGAATg                                                                                  >  2:553392/1‑140 (MQ=255)                                                    agccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCAGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                  >  2:950974/1‑140 (MQ=255)                                                     gccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGa                                                                                 <  2:1585621/140‑1 (MQ=255)                                                     gccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGa                                                                                 <  2:141416/140‑1 (MQ=255)                                                     gccgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCAGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGa                                                                                 <  1:950974/140‑1 (MQ=255)                                                       cgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAc                                                                               <  1:412948/140‑1 (MQ=255)                                                       cgaagcAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAc                                                                               <  2:1760291/140‑1 (MQ=255)                                                       cgaagcAAACGGCCAGGACAACCCCC‑‑AGAGTTCCCTGGAAGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAc                                                                               <  2:630255/140‑1 (MQ=255)                                                       cgaagcAAACGGCCACGGCAACTCCC‑‑AGACTACCCGGCATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAc                                                                               <  1:591949/140‑1 (MQ=255)                                                        gaagcAAACGGCCAGGGCAACACCCAGAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGaa                                                                                <  1:1031582/140‑1 (MQ=255)                                                                           acacCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGaa                                                           <  2:1093682/140‑1 (MQ=255)                                                                            cacCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCCGAACGATCAGACCAGACACCGCAGCGaaa                                                          >  1:691487/1‑140 (MQ=255)                                                                            cacCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGaaa                                                          >  2:1265526/1‑140 (MQ=255)                                                                            cacCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGaaa                                                          >  2:1901375/1‑140 (MQ=255)                                                                              ccc‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAaaga                                                        <  1:1878539/140‑1 (MQ=255)                                                                                   agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGc                                                     >  2:1800770/1‑140 (MQ=255)                                                                                   agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGc                                                     >  2:1372321/1‑140 (MQ=255)                                                                                     agTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCaa                                                   <  1:1265526/140‑1 (MQ=255)                                                                                       tACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAca                                                 <  1:362505/140‑1 (MQ=255)                                                                                        aCCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGTATGAACAAACCGGAGACCGCAGCGAAAGAAGCAAcag                                                >  1:625089/1‑140 (MQ=255)                                                                                         cccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagc                                               >  1:1114690/1‑140 (MQ=255)                                                                                          ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                              >  2:1710299/1‑140 (MQ=255)                                                                                          ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                              <  1:1372321/140‑1 (MQ=255)                                                                                               aTGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACGGACCAGAGACCGCAGCGAAAGGAGCAAcagcggtcag                                         >  1:554241/1‑140 (MQ=255)                                                                                                 gaagaaCGGATGGTTGACGACGCATTTACCGCTACTGGTGGTGCCCGTCTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcggtcagcg                                       <  2:554241/140‑1 (MQ=255)                                                                                                 gaagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcggtcagcg                                       >  2:1228487/1‑140 (MQ=255)                                                                                                 gaagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcggtcagcg                                       <  1:1632312/140‑1 (MQ=255)                                                                                                 gaagaaCGGATGGTTCAAGACGCATCTACTGATACGGTTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcggtcagcg                                       <  1:587071/140‑1 (MQ=255)                                                                                                          aTGGTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACGCCGAAGCGAAAGAAGCGACAGGGGTCAGCGGTGAAACGt                              >  2:536871/1‑140 (MQ=255)                                                                                                            ggTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTc                            >  1:1852741/1‑140 (MQ=255)                                                                                                            ggTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTc                            >  1:1432771/1‑140 (MQ=255)                                                                                                            ggTTGAAGACGAATTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTc                            >  2:154094/1‑140 (MQ=255)                                                                                                               tGAAGACGAATTTACCGTTACGTGTAGTGCCTGTGTCATTCATCTGTATCGCAGCAACCGGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGt                         <  2:691487/140‑1 (MQ=255)                                                                                                                      gAATTTACCGATACGAGTAGTGCCCATGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCagag                  <  1:536871/140‑1 (MQ=255)                                                                                                                        aTTTACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGcc                >  2:371052/1‑140 (MQ=255)                                                                                                                           tACCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATc             <  1:371052/140‑1 (MQ=255)                                                                                                                            aCCGATACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCg            <  2:1114690/140‑1 (MQ=255)                                                                                                                                aTACGGGTAGTGCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCAt        >  1:1107952/1‑140 (MQ=255)                                                                                                                                      gtagtgCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGc  >  2:331987/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       GGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCC‑‑AGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGC  >  NC_000913/4365447‑4365717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑