Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,480	T→C	noncoding (25/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,480	0	T	C	98.7%	250.3 / ‑4.6	74	noncoding (25/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (39/34);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

TTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCC  >  NC_000913/696344‑696615                                                                                                                                         |                                                                                                                                        ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGcc                                                                                                                                      <  2:1246575/140‑1 (MQ=255)  tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGCGCCt                                                                                                                                     >  1:22565/1‑140 (MQ=255)    aaGGATAAGACGTGTCACCATCTCATTCGACCTTGCATGTACGCAGAACAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                                                                                                   <  2:496888/140‑1 (MQ=37)         tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTTGGACTGCCGGAATCAGAATCCGGCGCCTTACCGCt                                                                                                                              >  2:718111/1‑140 (MQ=255)         tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCt                                                                                                                              >  1:112799/1‑140 (MQ=255)         tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCt                                                                                                                              >  1:1300832/1‑140 (MQ=255)          aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCtt                                                                                                                             >  1:1314067/1‑140 (MQ=255)          aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGCACGAGGATTCGAACCTCGGAATGGCGGAATCAGAATCGGGCGCCTTACCGCtt                                                                                                                             >  2:58753/1‑140 (MQ=255)            gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTgg                                                                                                                           >  1:1595732/1‑140 (MQ=255)               gtgtCAACATCGCATTGTACATTTAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  2:850500/140‑1 (MQ=255)               gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  1:424780/140‑1 (MQ=255)               gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  1:1598284/140‑1 (MQ=255)               gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  1:1878662/140‑1 (MQ=255)               gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  2:112799/140‑1 (MQ=255)               gtgtCAACATCGCATTCGACATCGAATGAGCGCAGAAGAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGa                                                                                                                        <  1:599232/140‑1 (MQ=255)                 gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATa                                                                                                                      >  1:399533/1‑140 (MQ=255)                 gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATa                                                                                                                      >  2:430936/1‑140 (MQ=255)                  tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTTGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATAc                                                                                                                     >  1:1466781/1‑140 (MQ=255)                  tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATAc                                                                                                                     <  1:1763860/140‑1 (MQ=255)                  tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATAc                                                                                                                     >  1:1224289/1‑140 (MQ=255)                   cAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCAGGCGCCTTACCGCTTGGCGATAcc                                                                                                                    <  2:85426/140‑1 (MQ=255)                      cATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCa                                                                                                                 >  1:70388/1‑140 (MQ=255)                       aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTCAGAATTCGGCGCTTTACCGCTTGGCGATACCCCaa                                                                                                                >  1:654302/1‑140 (MQ=255)                        tCGCATTCGACATTGTATGAACGCACAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAAc                                                                                                               <  1:811922/140‑1 (MQ=255)                        tCGCATTCGACATTGTATGAACCCAGAAACGCAAAAATCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAAc                                                                                                               <  1:859376/140‑1 (MQ=21)                         cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACt                                                                                                              >  1:1253248/1‑140 (MQ=255)                         cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACt                                                                                                              >  1:46691/1‑140 (MQ=255)                           cATTCGACATTGAATGATCGCAAAAAAGCAACAAGCTCGCCGAAGCGATCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                            <  2:654302/140‑1 (MQ=21)                            aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                           >  2:114386/1‑140 (MQ=255)                              tCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                         <  1:1234868/140‑1 (MQ=255)                                    ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                                                                                                   <  2:46691/140‑1 (MQ=255)                                     tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                         >  1:212746/1‑133 (MQ=255)                                     tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                         <  2:212746/133‑1 (MQ=255)                                        atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                         <  1:1916295/130‑1 (MQ=255)                                        atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                         >  2:1916295/1‑130 (MQ=255)                                            aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTaa                                                                                           >  2:1801057/1‑140 (MQ=255)                                            aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                                           <  1:58753/140‑1 (MQ=255)                                                  aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGACCAAGGATTCGAACCTCGGATTGCCGAAATAAGATTCCGGCGCCTTCCCGTTTGGCGATCCCCCAACTGGTGGCCCTTAAAAGGAAACGGTCt                                                                                     >  2:614444/1‑140 (MQ=255)                                                    aaGCAAAAAGCTCGCCGAAGCGAGGTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTCCGCCTCCGGTGCCTTACTGTTTGGCGTTTCCCCAGCTGGGTGCACTGATAAGGTAAGCgggtgg                                                                                   <  2:1489977/140‑6 (MQ=21)                                                       cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGGAAGCGCATTGAAt                                                                                >  2:989822/1‑140 (MQ=255)                                                             gCTCGCCGAAGCGAGCTTTTTTCATGTGGACGGGGTATGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                          <  1:614444/140‑1 (MQ=25)                                                               tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                                        <  2:22565/140‑1 (MQ=255)                                                               tCGCCGAAGCGAGCTTTCTTAATGTGGCTGGCGTACGAGAATTCGAACTTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                                        <  2:506757/140‑1 (MQ=25)                                                                cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCt                                                                       >  1:1451197/1‑140 (MQ=255)                                                                cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACAGGGCGCAATTACAAGGAAAGCATCTTGAATAAATTGGCt                                                                       >  2:552761/1‑140 (MQ=21)                                                                 gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAATGTGAGCGTCTTGAATAAATTGGCTg                                                                      >  1:506757/1‑140 (MQ=255)                                                                       gCGAGCTTTTTTAATCTGGCTGCGGCACGCGGATTCGCACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                                <  1:552761/140‑1 (MQ=25)                                                                        cGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACg                                                               <  2:399533/140‑1 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGGATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                       <  2:1889965/131‑1 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCATTTACAAGGTAAGCGTCGTGAGTAAATTGTCTGGTGTACCAGGCTTCGAACCTCGGa                                              >  2:525157/1‑140 (MQ=21)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGACCCTCGGa                                              >  2:444172/1‑140 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                       >  1:1889965/1‑131 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGa                                              >  1:1256510/1‑140 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGa                                              >  1:1603983/1‑140 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGa                                              >  2:302259/1‑140 (MQ=255)                                                                                         gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGCCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGa                                              >  1:1036871/1‑140 (MQ=255)                                                                                           tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAAAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaaa                                            >  2:1311025/1‑139 (MQ=255)                                                                                               gTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCTGCGCCTTACCGCTTGGCGATACCCAAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                        <  2:1224289/140‑1 (MQ=255)                                                                                                  cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                                     <  2:990942/140‑1 (MQ=255)                                                                                                    aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc                                   >  1:258445/1‑140 (MQ=255)                                                                                                        ttCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                               >  1:1715451/1‑140 (MQ=255)                                                                                                         tCGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                              >  2:1214343/1‑140 (MQ=255)                                                                                                          gagacagcGGAATGCCGGAATCCGATTCCTGCGCCTTTCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                             <  1:497043/133‑1 (MQ=37)                                                                                                          cGAACCTCGGAATGCCGGAATCCGATTCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                             <  1:718111/140‑1 (MQ=255)                                                                                                          cGAACCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                             <  2:70388/140‑1 (MQ=255)                                                                                                            aaCCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                          >  1:265428/1‑125 (MQ=255)                                                                                                            aaCCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                          <  2:265428/125‑1 (MQ=255)                                                                                                             aCCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                          <  2:1253248/140‑1 (MQ=255)                                                                                                             aCCTCGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTAACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                          <  1:430936/140‑1 (MQ=255)                                                                                                                 cGGAATGCCGGAATCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                      >  1:60118/1‑140 (MQ=255)                                                                                                                            aaTCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCGCGGGATGGCGGAATCAGAATCGGGTGCCTTAcctgtctc           >  2:497043/1‑134 (MQ=38)                                                                                                                            aaTCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg           >  2:1671403/1‑140 (MQ=255)                                                                                                                             aTCAGAATCCGGCGCCTTAGCGATTGGCGATACGCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc          <  2:1595732/140‑1 (MQ=255)                                                                                                                              tCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                           <  2:1415647/122‑1 (MQ=255)                                                                                                                              tCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                           >  1:1415647/1‑122 (MQ=255)                                                                                                                              tCAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg         >  1:1411840/1‑140 (MQ=255)                                                                                                                               cAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGa        <  1:302259/140‑1 (MQ=255)                                                                                                                                    tCCGGCGCCTTACCGCTTGGCGCTACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAccc   <  1:699006/140‑1 (MQ=255)                                                                                                                                     ccGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAcccc  <  2:258445/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCC  >  NC_000913/696344‑696615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑