Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	727,833	C→T	W300* (TGG→TGA)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	727,833	0	C	T	100.0%	245.2 / NA	72	W300* (TGG→TGA)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/37);  total (35/37)

GCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATC  >  NC_000913/727696‑727968                                                                                                                                          |                                                                                                                                        gcgcCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCGGTGCCAGGAGACGACGATTACGCTGAACTTCTGCTca                                                                                                                                       >  1:916590/1‑140 (MQ=255)       caGGGAGCCGCCCGCGTTCCGGCCGCAAACACGCTCCTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATcac                                                                                                                                 <  2:530811/140‑1 (MQ=255)       caGGAAGCCGCCGTCGTGACGAGCGCAAACAAGCTGCTGGCCAGCACGGCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTGTGCTCACATcac                                                                                                                                 <  2:809998/140‑1 (MQ=255)        aGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTcac                                                                                                                                      >  2:1282347/1‑134 (MQ=255)        aGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTcac                                                                                                                                      <  1:1282347/134‑1 (MQ=255)         ggAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATcacca                                                                                                                               <  2:1734438/140‑1 (MQ=255)         ggAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATcacca                                                                                                                               <  2:1610512/140‑1 (MQ=255)             gccgccGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGACGAGGATTACCCCGAACTTCTGGTCACATCACCACGcc                                                                                                                           >  1:932697/1‑140 (MQ=255)              ccgccgTCGTCACGACCGCAAAAAGCCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCt                                                                                                                          <  1:521141/140‑1 (MQ=255)               cgccgTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACAt                                                                                                                                    <  2:1141401/129‑1 (MQ=255)               cgccgTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACAt                                                                                                                                    >  1:1141401/1‑129 (MQ=255)                   gtcgtcACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGTCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCa                                                                                                                     <  2:1842604/140‑1 (MQ=255)                                          aCTGACCAGCACGCCGAAACGGCTCGCTTAACCTTCCCTATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCg                                                                                              <  1:843387/140‑1 (MQ=255)                                               ccAGCACGCCGAAACGGCTCTCCTTACCTACCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCcac                                                                                         <  1:618645/140‑1 (MQ=255)                                                 aGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTAGGTAGATGACAAAAATGACTGACATCGCCCACAg                                                                                       >  2:843387/1‑140 (MQ=255)                                                   cACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCAACAGCa                                                                                     >  2:1307692/1‑140 (MQ=255)                                                     cGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAAc                                                                                   >  1:1100389/1‑140 (MQ=255)                                                                          cTTCCATATTGATGCTGATGTCCGTGCCCAGTGCCCGCACATGAGGATCACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCg                                                              <  2:691953/140‑1 (MQ=255)                                                                            tCCATATTGATGCTGTTGTCCGGGCTCAGAGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGcc                                                            <  1:684639/140‑1 (MQ=255)                                                                             ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGccc                                                           >  1:38500/1‑140 (MQ=255)                                                                             ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGccc                                                           <  1:274238/140‑1 (MQ=255)                                                                             ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGccc                                                           >  1:1236518/1‑140 (MQ=255)                                                                             ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGccc                                                           >  1:1103070/1‑140 (MQ=255)                                                                             ccATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGccc                                                           >  2:274238/1‑140 (MQ=255)                                                                                 atTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCa                                                       >  1:74462/1‑140 (MQ=255)                                                                                   tGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                     >  2:1250242/1‑140 (MQ=255)                                                                                   tGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                     <  2:1103070/140‑1 (MQ=255)                                                                                   tGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                     <  1:1250242/140‑1 (MQ=255)                                                                                   tGATGCTGCTGTCCGTGCCCAGCGCCAGCTGATGAGGCTTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACt                                                     <  1:531920/140‑1 (MQ=255)                                                                                      tgctgcTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACACCAACATGCGCCCCTCGCGGCGATCGCCCATCACTTCa                                                  >  1:692372/1‑140 (MQ=255)                                                                                       gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACTTCGCCCACAGCAACATGCGCCCCTGACGGCTATCGCCCATCACTTCAc                                                 >  2:684639/1‑140 (MQ=255)                                                                                       gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACGGCATCATTCGCCCCTGGCGGCGATCGCCCATCACTTCAc                                                 >  2:623543/1‑140 (MQ=255)                                                                                       gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGa                                                                 <  1:970865/124‑1 (MQ=255)                                                                                       gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGa                                                                 >  2:970865/1‑124 (MQ=255)                                                                                       gctgctGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCAc                                                 >  1:408173/1‑140 (MQ=255)                                                                                        ctgctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCACCCATCACTTCAcc                                                >  2:1614599/1‑140 (MQ=255)                                                                                          gctgTCCGTGCCCAGTGCCAGCCGATGAGTATTACCCAGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                              <  1:623543/140‑1 (MQ=255)                                                                                          gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaa                                              <  2:1078028/140‑1 (MQ=255)                                                                                          gctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCCCCCACAGCAACAAGCGCCCCTGGCGCCGATACCCCATCACTTCACCaa                                              >  2:876386/1‑140 (MQ=255)                                                                                           ctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaaa                                             >  1:429402/1‑140 (MQ=255)                                                                                           ctgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCaaa                                             >  2:1417921/1‑140 (MQ=255)                                                                                             gTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAgg                                           >  2:1422566/1‑140 (MQ=255)                                                                                               ccGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCa                                         <  2:38500/140‑1 (MQ=255)                                                                                                cGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCaa                                        >  2:90014/1‑140 (MQ=255)                                                                                                cGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCa                                         <  1:176396/139‑1 (MQ=255)                                                                                                cGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCa                                         >  2:176396/1‑139 (MQ=255)                                                                                                   gCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGc                                     >  1:1759310/1‑140 (MQ=255)                                                                                                   gCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGc                                     >  2:1750502/1‑140 (MQ=255)                                                                                                   gCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGc                                     >  2:87299/1‑140 (MQ=255)                                                                                                    cccAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGca                                    <  2:1236518/140‑1 (MQ=255)                                                                                                    cccAGTGCCAGCAGACGAGGATCACGCTGAACTTCTGTTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGca                                    <  1:876386/140‑1 (MQ=255)                                                                                                      cAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGcaca                                  <  1:1422566/140‑1 (MQ=255)                                                                                                       aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                 <  2:1100389/140‑1 (MQ=255)                                                                                                       aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                 <  1:87299/140‑1 (MQ=255)                                                                                                       aGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCAACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                 <  1:1307692/140‑1 (MQ=255)                                                                                                         tGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                               <  1:1750502/140‑1 (MQ=255)                                                                                                         tGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                               <  2:1759310/140‑1 (MQ=255)                                                                                                         tGCCAGCAGATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAgcg                               <  2:1345353/140‑1 (MQ=255)                                                                                                             agcagATGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGt                           <  2:408173/140‑1 (MQ=255)                                                                                                                 gATGAGGATTACCCTGATCTTCTGCTCACATTACCTCGCCTACGCAGAAGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTggg                       <  2:692372/140‑1 (MQ=255)                                                                                                                 gATGAGGAGTACCCTGCCCTTCCGCTCACAGAAGCCCGCCTACGCAGACGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTggg                       <  2:916590/140‑1 (MQ=255)                                                                                                                   tGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                 <  1:1033061/128‑1 (MQ=255)                                                                                                                   tGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAg                                 >  2:1033061/1‑128 (MQ=255)                                                                                                                   tGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGtt                          <  2:1001500/135‑1 (MQ=255)                                                                                                                   tGAGGATTACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGtt                          >  1:1001500/1‑135 (MQ=255)                                                                                                                         ttACCCTGGACTTCTGCTCACATCACCACGTCTACCCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCaagaa               <  2:932697/140‑1 (MQ=255)                                                                                                                          tACCCTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCaagaag              <  1:1417921/140‑1 (MQ=255)                                                                                                                            cccTGAACTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGAt            >  2:199120/1‑140 (MQ=255)                                                                                                                                  aCTTCTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAg      >  2:1850998/1‑140 (MQ=255)                                                                                                                                      cTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATc  >  1:1842012/1‑140 (MQ=255)                                                                                                                                      cTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATc  >  1:1452387/1‑140 (MQ=255)                                                                                                                                      cTGCTCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATc  >  2:98533/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATC  >  NC_000913/727696‑727968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑