Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,128,274	C→T	L146L (CTG→TTG)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,128,274	0	C	T	100.0%	317.8 / NA	92	L146L (CTG→TTG)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/46);  total (46/46)

GCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTG  >  NC_000913/1128139‑1128410                                                                                                                                        |                                                                                                                                         gCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCACTATATCTTGCTGATCTCCCTGGCGTCGTtggt                                                                                                                                      <  1:1079196/140‑1 (MQ=255)      cATGCTCGCCGCACCTTGGGTGCTCCTGGAGACCGCGACAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAg                                                                                                                                 <  1:577699/140‑1 (MQ=255)      cATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAg                                                                                                                                 <  1:438485/140‑1 (MQ=255)           tCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGAtt                                                                                                                            <  1:1601230/140‑1 (MQ=255)               cgcACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGa                                                                                                                        >  1:747253/1‑140 (MQ=255)                    cGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACg                                                                                                                   >  1:434228/1‑140 (MQ=255)                            aTCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCg                                                                                                           <  2:1449623/140‑1 (MQ=255)                            aTCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCg                                                                                                           >  2:1506077/1‑140 (MQ=255)                              cATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGa                                                                                                                        <  1:366331/125‑1 (MQ=255)                              cATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGa                                                                                                                        >  2:366331/1‑125 (MQ=255)                                tGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTgg                                                                                                                <  1:1002913/131‑1 (MQ=255)                                tGGTGACCGCGCCAGGCTTCGCTGACACAGCTGAAAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTgg                                                                                                                >  2:1002913/1‑131 (MQ=255)                                 ggTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTtct                                                                                                      >  1:1683706/1‑140 (MQ=255)                                  gTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTtctc                                                                                                     >  1:1147173/1‑140 (MQ=255)                                  gTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTtctc                                                                                                     >  2:6458/1‑140 (MQ=255)                                   tGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAAc                                                                                                             <  2:1752271/131‑1 (MQ=255)                                   tGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAAc                                                                                                             >  1:1752271/1‑131 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTc                                                                                                >  1:992834/1‑140 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTc                                                                                                >  1:1770852/1‑140 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTc                                                                                                >  1:1165173/1‑140 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTc                                                                                                >  1:1215327/1‑140 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAACACGTAAAACCGCTTCTCAATTc                                                                                                >  2:855145/1‑140 (MQ=255)                                       cgcgCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGACTCTCAATACGTGGGACCGCTTCTCGATTc                                                                                                >  1:913650/1‑140 (MQ=255)                                         cgcCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATTCGTGGAACCGCTTCTCGATTCCg                                                                                              >  2:1556489/1‑140 (MQ=255)                                         cgcCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGAAGCGATTCTGACTACGTGGAAGCGCTTCTCGATTCCg                                                                                              >  1:889462/1‑140 (MQ=255)                                         cgcCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCATCGTTGGTGGGACCGATTCTGAATAGGTGGAACCGCTTCTCGATTCCg                                                                                              >  2:687636/1‑140 (MQ=255)                                            cAGGCTTCGCTGACACAGCTGACAATTTTGCCCTGACTAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCAATTCCGGCg                                                                                           <  1:855145/140‑1 (MQ=255)                                              ggCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGtt                                                                                         <  1:1506077/140‑1 (MQ=255)                                              ggCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGtt                                                                                         <  2:1683706/140‑1 (MQ=255)                                                 ttCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGc                                                                                      <  2:747253/140‑1 (MQ=255)                                                     cTGACACAGCTGACACATTTGCCCTAACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCa                                                                                  <  1:687636/140‑1 (MQ=255)                                                       gACACAGCTGACAAATTTGCCCAGACCAGCCGGTTTCTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAac                                                                                <  2:913650/140‑1 (MQ=255)                                                         cacaGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAacac                                                                              <  2:1165173/140‑1 (MQ=255)                                                         cacaGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAacac                                                                              <  2:434228/140‑1 (MQ=255)                                                            aGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGc                                                                           <  2:1215327/140‑1 (MQ=255)                                                            aGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGc                                                                           <  2:1770852/140‑1 (MQ=255)                                                             gCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATTCGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCt                                                                          <  1:1556489/140‑1 (MQ=255)                                                              cTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCtt                                                                         >  1:1612690/1‑140 (MQ=255)                                                              cTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCtt                                                                         <  2:1874555/140‑1 (MQ=255)                                                                       ttGCCCTGTCCAGCCAGTTACTTAAGATTACCTTTGCCTTTATCTTGCGGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGc                                                                <  2:889462/140‑1 (MQ=255)                                                                       ttGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGc                                                                <  2:992834/140‑1 (MQ=255)                                                                             tGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCGGGCGTTTGCTCGAAAACTGCTCAACATCAGCATGAtt                                                          >  1:500980/1‑140 (MQ=255)                                                                               aCCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTgg                                                        >  1:1421955/1‑140 (MQ=255)                                                                                ccagccagCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGt                                                       >  1:371529/1‑140 (MQ=255)                                                                                 cagccagcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGtt                                                      <  2:1612690/140‑1 (MQ=255)                                                                                      agcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTcgcg                                                 <  2:371529/140‑1 (MQ=255)                                                                                         tactaAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTg                                              >  1:1363087/1‑140 (MQ=255)                                                                                           ctaAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGtt                                            >  1:363077/1‑140 (MQ=255)                                                                                              aaGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGc                                         >  2:1093320/1‑140 (MQ=255)                                                                                                 aTTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGATCCAACACGGCTGAACGTCAGCATGATTGGATTCGCGCTGTTTGCCGc                                      >  2:608146/1‑140 (MQ=255)                                                                                                 aTTACCTTTCCCTATATCTTGCGGATCTCCCTGGCGTCGTTGGTGGGAACGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCA‑‑‑‑GATTGGTTTCGCGCTGTTTGCCGCACCg                                  <  2:816521/140‑1 (MQ=255)                                                                                                  ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTcgcg                                                 >  2:1893242/1‑128 (MQ=255)                                                                                                  ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTcgcg                                                 <  1:1893242/128‑1 (MQ=255)                                                                                                  ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCa                                     >  1:1240096/1‑140 (MQ=255)                                                                                                  ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCa                                     >  1:336/1‑140 (MQ=255)                                                                                                   tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGTCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAc                                    <  1:6458/140‑1 (MQ=255)                                                                                                   tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAc                                    >  1:412927/1‑140 (MQ=255)                                                                                                   tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAc                                    >  2:440396/1‑140 (MQ=255)                                                                                                    aCCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAcc                                   <  2:1257114/140‑1 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAcc                                   <  2:1543958/139‑1 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAcc                                   >  1:1543958/1‑139 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  <  1:440396/140‑1 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  >  2:740255/1‑140 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  <  2:336/140‑1 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  <  2:1147173/140‑1 (MQ=255)                                                                                                     ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCACCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  >  2:740279/1‑140 (MQ=255)                                                                                                     ccTTTCCCTATATCTAGCTGATATCGCTGACGTCGTTAGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                  <  1:711906/140‑1 (MQ=255)                                                                                                       tttCCCTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTa                                <  2:1240096/140‑1 (MQ=255)                                                                                                          cccTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGcc                                        >  2:936847/1‑129 (MQ=255)                                                                                                          cccTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGcc                                        <  1:936847/129‑1 (MQ=255)                                                                                                          cccTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACtt                             <  1:44003/140‑1 (MQ=255)                                                                                                          cccTATATCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACtt                             >  2:115818/1‑140 (MQ=255)                                                                                                               tatCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTGACATCAGCTTGATTGGTTTCGCGCTCTTTGTCGCACCGTACTTTAAcc                        >  2:711906/1‑140 (MQ=255)                                                                                                               tatCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAAcc                        <  1:115818/140‑1 (MQ=255)                                                                                                                atCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTTGTTTCGCGCTGTTTGCCGCACCGTa                                <  2:1308928/131‑1 (MQ=255)                                                                                                                atCTTGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTa                                >  1:1308928/1‑131 (MQ=255)                                                                                                                    tGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCg                   >  2:1209398/1‑140 (MQ=255)                                                                                                                    tGCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGATGTCTGCCGCACCCTACTTTAACCCActg                   >  1:824292/1‑138 (MQ=255)                                                                                                                     gCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATTGTTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCgg                  >  2:314444/1‑140 (MQ=255)                                                                                                                     gCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCgg                  >  2:1597025/1‑140 (MQ=255)                                                                                                                     gCTGATCTCCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCgg                  >  1:126850/1‑140 (MQ=255)                                                                                                                           ctcCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctgg            >  1:1170443/1‑140 (MQ=255)                                                                                                                           ctcCCTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCATACTTTAACCCACCGGTgctgg            >  1:746032/1‑140 (MQ=255)                                                                                                                             cccTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcg          <  2:363077/140‑1 (MQ=255)                                                                                                                              ccTGGCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgc         >  2:1374399/1‑140 (MQ=255)                                                                                                                                  gtgTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc     <  1:740255/138‑1 (MQ=255)                                                                                                                                  gCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc     <  1:740279/140‑1 (MQ=255)                                                                                                                                  gCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc     <  1:1209398/140‑1 (MQ=255)                                                                                                                                  gCGTCGTTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc     <  1:1597025/140‑1 (MQ=255)                                                                                                                                     tCGTTGGTGGGAGCGTTTCTGAATAGGTGTAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTg  <  1:657758/140‑1 (MQ=255)                                                                                                                                     tCGTTGGTGGGAGCGATTCTGACTACGTGGATCCGCTTCGCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTg  <  2:750532/140‑1 (MQ=255)                                                                                                                                     tCGTTGGTGGGAGCGATTCTGAATACGTGGATCCGATTCACGATTCCGTCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTg  <  2:651657/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTG  >  NC_000913/1128139‑1128410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑