Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,132,112	A→G	S87G (AGC→GGC)	flgD →	flagellar biosynthesis, initiation of hook assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,132,112	0	A	G	100.0%	299.3 / NA	83	S87G (AGC→GGC)	flgD	flagellar biosynthesis, initiation of hook assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/43);  total (40/43)

CAGCTGAAAAACCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACAGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGC  >  NC_000913/1131977‑1132248                                                                                                                                        |                                                                                                                                         gacaggAAAAACCAGTACCCGAACAATCCGATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCa                                                                                                                                      <  1:754620/135‑1 (MQ=255) cAGCTGAAAAACCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCa                                                                                                                                      <  2:295317/140‑1 (MQ=255)  aGCTGAAAAACCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCAGGCTCGGACCTAATTCCGGACATATTGATAACGGCCAg                                                                                                                                     >  1:924407/1‑140 (MQ=255)   gCTGAAAAACCAGGACCGGACCAATCCAATGAAAAACAAGGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGt                                                                                                                                    <  2:757427/140‑1 (MQ=255)   gCTGAAAAACCAGGACCCGACCCATCCAATGCAAATCAACGAGCTGACATCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGt                                                                                                                                    <  2:505975/140‑1 (MQ=255)   gCTGAAAAACCAGGAACCGACCAATTCAACGGAAAACCACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGt                                                                                                                                    <  1:607006/140‑1 (MQ=255)        agaaCCAGGACCCGACCAATCCTATAGAACACAACGATCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTa                                                                                                                               <  1:577958/138‑1 (MQ=255)        aaaaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTa                                                                                                                               <  1:1392682/140‑1 (MQ=255)          aaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACa                                                                                                                             <  1:3143/140‑1 (MQ=255)          aaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACa                                                                                                                             <  1:218354/140‑1 (MQ=255)          aaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACa                                                                                                                             <  2:1433916/140‑1 (MQ=255)          aaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACa                                                                                                                             <  2:1444298/140‑1 (MQ=255)          aaCCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACa                                                                                                                             <  1:1361528/140‑1 (MQ=255)              aGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGTCCAGTCGTTACAGGcc                                                                                                                         <  2:106525/140‑1 (MQ=255)              aGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGcc                                                                                                                         <  2:1824836/140‑1 (MQ=255)                 aCCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTGGGATCTATTTCCAGACTGATTGATAGCGGCCAGTCGTTACAGGctgtc                                                                                                                      >  2:754620/1‑136 (MQ=255)                 aCCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGt                                                                                                                      >  1:1403210/1‑140 (MQ=255)                     gACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCAGACAGATTGATAACGGACAGTGGTTACAGGCCAGTAAct                                                                                                                  >  2:577958/1‑139 (MQ=255)                      aCCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCt                                                                                                                 >  1:1572341/1‑140 (MQ=255)                      aCCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCt                                                                                                                 >  1:32555/1‑140 (MQ=255)                             caatGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGt                                                                                                          <  1:1481691/140‑1 (MQ=255)                               atGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCa                                                                                                        <  2:99400/140‑1 (MQ=255)                               atGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAACACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCa                                                                                                        <  2:1566441/140‑1 (MQ=255)                               atGGAAAACAACGAGATGACGTAGCGATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCa                                                                                                        <  1:866662/140‑1 (MQ=255)                                tGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCAc                                                                                                       >  1:1751708/1‑140 (MQ=255)                                 ggAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCATGGTCAGCGGGATTGAAAAGCTGAATAGCACGCTCGGATCTATTTCAGGATAGATGGAGAACGGCCAGTCGTTACAGTCCAGTACCCTGATCGGTCACg                                                                                                      <  2:1907396/140‑1 (MQ=255)                                 ggAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACg                                                                                                      <  2:1767417/140‑1 (MQ=255)                                   aaaaCAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGc                                                                                                    <  2:1814710/140‑1 (MQ=255)                                     aacaacGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGt                                                                                                  >  1:378750/1‑140 (MQ=255)                                     aacaacGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGt                                                                                                  >  1:1465269/1‑140 (MQ=255)                                     aacaacGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGACCAGTAACCTGATCGGTCACGGCGt                                                                                                  >  1:642708/1‑140 (MQ=255)                                     aacaacGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCAAGTCGTTTCAGACCCGTAACCAGATCGGTCACGGCGt                                                                                                  >  1:718612/1‑140 (MQ=255)                                     aacaacGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGAATGATAACGGCCAGTCGTTACAGGCCAGTAAACTGATCGGACACGGCGt                                                                                                  >  2:866662/1‑140 (MQ=255)                                          cGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATGACGGCCAGGCGTAACAGGCCAGTAAGCTGACCGGTCACGGCGtgatga                                                                                             >  2:661100/1‑140 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACg                                                                                                      <  1:1473772/129‑1 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACg                                                                                                      >  2:1473772/1‑129 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATc                                                                                           <  2:32555/140‑1 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATc                                                                                           <  2:1751708/140‑1 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATc                                                                                           >  1:1760172/1‑140 (MQ=255)                                            aGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATc                                                                                           <  2:1572341/140‑1 (MQ=255)                                             gCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATcc                                                                                          >  1:1634574/1‑140 (MQ=255)                                             gCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAAACTGATCGGTTACGGCGTGATGATcc                                                                                          >  1:843704/1‑140 (MQ=255)                                                 aCGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCgg                                                                                      <  2:1634574/140‑1 (MQ=255)                                                   gTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGca                                                                                    <  2:1760172/140‑1 (MQ=255)                                                      gCAATTGGCACAAATCATCACGGTCAGCGGGTTTGAAAAACTCAATACGACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGcacca                                                                                 <  2:921986/140‑1 (MQ=255)                                                      gCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGcac                                                                                   <  2:1724736/138‑1 (MQ=255)                                                      gCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGcac                                                                                   >  1:1724736/1‑138 (MQ=255)                                                         aTTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTg                                                                              <  1:1464893/140‑1 (MQ=255)                                                           tGGCACAAATCAGCACGGTCAGCGGGATTGATAAATTCAATACCACGCGCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGtt                                                                            <  2:718612/140‑1 (MQ=255)                                                               acaAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTg                                                                        <  1:202657/140‑1 (MQ=255)                                                                         aCGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCgg                                                              <  2:1465269/140‑1 (MQ=255)                                                                           ggTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCa                                                            >  2:1220623/1‑140 (MQ=255)                                                                             tCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGt                                                          >  2:1384923/1‑140 (MQ=255)                                                                             tCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGt                                                          >  1:1478431/1‑140 (MQ=255)                                                                             tCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGt                                                          >  2:27415/1‑140 (MQ=255)                                                                             tCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGACACGGCGTGATGGTCCACGGCACCACTGTTATTGCGGGAACCGGCAgg                                                          >  2:782000/1‑139 (MQ=255)                                                                               aGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCCCGGCGTGACGATTCCCGGCACCACTTTTCTAGCGGGAACCGGCAGTga                                                        >  2:778102/1‑140 (MQ=255)                                                                                gCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTgaa                                                       >  2:1595567/1‑140 (MQ=255)                                                                                         acagaCTCGATACCACTGTCGGTTCTATTTCGGCACAGATTGATAACGTCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCt                                              <  2:861135/136‑1 (MQ=255)                                                                                         aaaaaCTCAATACCACGATCGGATCTATTTCCGGATAGACTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCt                                              <  2:645362/140‑1 (MQ=255)                                                                                           aaaCTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCtgt                                            <  1:1763384/140‑1 (MQ=255)                                                                                              cTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTgac                                         >  1:1559331/1‑140 (MQ=255)                                                                                                cAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTgacca                                       >  1:253060/1‑140 (MQ=255)                                                                                                 aaTACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTgaccac                                      >  2:1664620/1‑140 (MQ=255)                                                                                                   tACCACGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTAGCGGGAACCGGCAGCGAAGAATGGGCTGTgaccacga                                    >  1:852339/1‑140 (MQ=255)                                                                                                        cGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCTGTGAAGTAGGGGCCGTgaccacgacctgt                               >  1:861135/1‑137 (MQ=255)                                                                                                        cGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGaccacgaccacg                               >  2:273320/1‑140 (MQ=255)                                                                                                        cGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGaccacgaccacg                               >  1:1406838/1‑140 (MQ=255)                                                                                                        cGCTCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGaccacgaccacg                               >  2:14699/1‑140 (MQ=255)                                                                                                           tCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCg                            >  1:1715615/1‑140 (MQ=255)                                                                                                           tCGGATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTCAAGGAGAGGCTGTTACCACGACCACGCCg                            >  1:751235/1‑140 (MQ=255)                                                                                                             ggATCTATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGtt                          <  2:157360/140‑1 (MQ=255)                                                                                                                  tATTTCCGGACAGATTGATAACGGCCAGTCTTTGCAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGgtg                     <  2:924407/140‑1 (MQ=255)                                                                                                                  tATTTCCGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGgtg                     <  2:378750/140‑1 (MQ=255)                                                                                                                    tttCCGGACAGATTGATTACGGTCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGtt                   <  2:642708/140‑1 (MQ=255)                                                                                                                       ccGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAg                >  2:377054/1‑140 (MQ=255)                                                                                                                       ccGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAg                >  1:1865851/1‑140 (MQ=255)                                                                                                                       ccGGACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCAAGACCACGCCGTTTGGTGTTGAg                >  1:821485/1‑140 (MQ=255)                                                                                                                         ggACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCt              >  2:244834/1‑140 (MQ=255)                                                                                                                          gACAGATTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTg             <  1:1664620/140‑1 (MQ=255)                                                                                                                            cAGATTGATAACGGCTAGTCGTTACTGGCAAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCa           <  1:778102/140‑1 (MQ=255)                                                                                                                               aTTGATAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACa        >  1:951964/1‑140 (MQ=255)                                                                                                                                   aTAACGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAggcg    <  1:27415/140‑1 (MQ=255)                                                                                                                                    tAACGGCCAGTCGTTTCAGGCCAGTCACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAggcgg   <  1:534655/140‑1 (MQ=255)                                                                                                                                     aaCGGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAggcggc  <  2:1865851/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CAGCTGAAAAACCAGGACCCGACCAATCCAATGGAAAACAACGAGCTGACGTCGCAATTGGCACAAATCAGCACGGTCAGCGGGATTGAAAAACTCAATACCACGCTCGGATCTATTTCCGGACAGATTGATAACAGCCAGTCGTTACAGGCCAGTAACCTGATCGGTCACGGCGTGATGATCCCCGGCACCACTGTTCTTGCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGC  >  NC_000913/1131977‑1132248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑