Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,241,020	A→G	V351A (GTG→GCG)	cvrA ←	putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,241,020	0	A	G	100.0%	247.5 / NA	67	V351A (GTG→GCG)	cvrA	putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/30);  total (37/30)

GGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATC  >  NC_000913/1240885‑1241148                                                                                                                                        |                                                                                                                                 ggCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGc                                                                                                                              <  2:1097004/140‑1 (MQ=255)     gAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGGGTGTTGTTCCCTACAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAgg                                                                                                                          <  2:792139/140‑1 (MQ=255)     gAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAgg                                                                                                                          >  1:1538429/1‑140 (MQ=255)      aaCCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGga                                                                                                                         >  1:1187690/1‑140 (MQ=255)       accaccACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGgat                                                                                                                        >  1:1301126/1‑140 (MQ=255)            cacTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTg                                                                                                                   >  2:1457485/1‑140 (MQ=255)                         ttAGCCGCCCACGAGAGTGATGTTCCCTGCACGAGGAGTGAAACCAGATCCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCAccgcgccgcg                                                                                                      <  2:682424/140‑1 (MQ=255)                            cccgccCACGAGAGTTATGTTCCCTGCAAGAGGGGGGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCTTTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTaa                                                                                                   <  2:1187690/139‑1 (MQ=255)                                     gagaGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCa                                                                                          <  1:320546/140‑1 (MQ=255)                                               ttCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAac                                                                                >  1:362466/1‑140 (MQ=255)                                                 cccTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAacac                                                                              >  2:1219274/1‑140 (MQ=255)                                                  ccTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACAcg                                                                             >  1:1162179/1‑140 (MQ=255)                                                    tGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCa                                                                                          >  1:1723815/1‑125 (MQ=255)                                                    tGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCa                                                                                          <  2:1723815/125‑1 (MQ=255)                                                    tGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACAcgcg                                                                           >  2:193402/1‑140 (MQ=255)                                                    tGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACAcgcg                                                                           >  2:1420100/1‑140 (MQ=255)                                                      cAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCt                                                                         >  1:1350122/1‑140 (MQ=255)                                                          agCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACg                                                                     <  1:1457485/140‑1 (MQ=255)                                                              gTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGa                                                                 >  1:1166248/1‑140 (MQ=255)                                                              gTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGa                                                                 >  1:213192/1‑140 (MQ=255)                                                                 aaaCCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACg                                                                     >  2:306250/1‑133 (MQ=255)                                                                 aaaCCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACg                                                                     <  1:306250/133‑1 (MQ=255)                                                                  aaCCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGa                                                             <  1:87836/140‑1 (MQ=255)                                                                   acaaGAACCACAAAGAGGGCGTCATTAAAGACCAGACGTGCATTCTCCACCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGaa                                                            <  2:114454/137‑1 (MQ=255)                                                                       gAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACcg                                                        <  2:1538429/140‑1 (MQ=255)                                                                             caAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAAACCTACCCAACTGGTAAACACGCGCTAACGCTGATTGAAACCGCGGAAg                                                  >  2:544877/1‑140 (MQ=255)                                                                             aaaaGAAGGCGACATTAAAAAACAGACGTGCATTCCCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAg                                                  <  2:1450608/139‑1 (MQ=255)                                                                                  aagGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAg                                             <  2:1166248/140‑1 (MQ=255)                                                                                  aagGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAg                                             <  2:241500/140‑1 (MQ=255)                                                                                      cGACATTAAAGAACAGACGTGCGTTCTCCGGCCCCGCGATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAAt                                         <  1:634401/140‑1 (MQ=255)                                                                                            tAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCa                                   >  1:1572023/1‑140 (MQ=255)                                                                                                   cAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCg                            >  1:1390985/1‑140 (MQ=255)                                                                                                   cAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACACAGATTGAAACCGCGGAAGGGGAg                                             <  2:1560473/123‑1 (MQ=255)                                                                                                   cAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACACAGATTGAAACCGCGGAAGGGGAg                                             >  1:1560473/1‑123 (MQ=255)                                                                                                   cAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGAGCTCACGCCGATTGAATCCGCGGAAAGGGAACAATCCAGCAAATACCg                            >  2:673313/1‑140 (MQ=255)                                                                                                     gACGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGaa                          >  2:1032542/1‑140 (MQ=255)                                                                                                      aCGTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGaaa                         >  1:1556882/1‑140 (MQ=255)                                                                                                        gTGCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGCAGGCGACCAATCCAGCAAATACCGAAAgg                       >  1:792730/1‑140 (MQ=255)                                                                                                          gCATTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAgggg                     >  1:354881/1‑140 (MQ=255)                                                                                                            aTTCTCCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGAc                   <  1:1032542/140‑1 (MQ=255)                                                                                                                tcCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGc               >  2:46741/1‑140 (MQ=255)                                                                                                                tcCAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGc               >  2:1714544/1‑140 (MQ=255)                                                                                                                  cAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  2:213192/140‑1 (MQ=255)                                                                                                                  cAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  2:362466/140‑1 (MQ=255)                                                                                                                  cAGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  2:1572023/140‑1 (MQ=255)                                                                                                                   aGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaa            <  2:1162179/140‑1 (MQ=255)                                                                                                                   aGCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaa            <  2:1556882/140‑1 (MQ=255)                                                                                                                   aGCCCCGCCATCATCGGAAACGAGGCCAGGAAGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaa            <  2:792425/140‑1 (MQ=255)                                                                                                                    gCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             >  1:1262275/1‑138 (MQ=255)                                                                                                                    gCCCCGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  2:1262275/138‑1 (MQ=255)                                                                                                                     ccccGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaaga          <  1:1756381/140‑1 (MQ=255)                                                                                                                      cccGCCATCATCGGGTACGCGGGCATGATGATTGGCAGCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaagaa         <  1:785340/140‑1 (MQ=255)                                                                                                                      cccGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCg              >  2:1283139/1‑135 (MQ=255)                                                                                                                      cccGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCg              <  1:1283139/135‑1 (MQ=255)                                                                                                                       ccGCCATCATCGGGAACGCGGCAAGGATGGTTGGCACGGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAat        <  1:758156/140‑1 (MQ=255)                                                                                                                        cGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGGGCATTCCAGCAAATACAGAAAAGGGACGGGCGAAGAata       >  2:758156/1‑140 (MQ=255)                                                                                                                        cGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGGAATCCAGGAAAGACGGAAAGGGGACGGGCGAAGAata       >  1:513646/1‑140 (MQ=255)                                                                                                                        cGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAat        >  2:469443/1‑139 (MQ=255)                                                                                                                        cGCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAat        <  1:469443/139‑1 (MQ=255)                                                                                                                         gCCATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAatat      <  1:1714544/140‑1 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGTAAGGGGAGTAATGCAGTAAATACCGAAAGGGGACGGGCGAAGAATatc     >  2:785340/1‑140 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             >  2:1816239/1‑132 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  1:1382193/132‑1 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             <  1:1816239/132‑1 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga             >  2:1382193/1‑132 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATatc     >  2:1478136/1‑140 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATatc     >  1:169243/1‑140 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATatc     >  2:34398/1‑140 (MQ=255)                                                                                                                          ccATCATCGGGAACGCGGCCAG‑‑‑GATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCAAGCAAATTCCGCAATGGGACGAGCGAAGAATatcatc  >  1:866918/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                 GGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATC  >  NC_000913/1240885‑1241148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑