Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,126 C→T R210R (CGC→CGT)  paaF → putative 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,1260CT100.0% 321.0 / NA 93R210R (CGC→CGT) paaFputative 2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/51);  total (42/51)

CTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGC  >  NC_000913/1457990‑1458261
                                                                                                                                            |                                                                                                                                       
cTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCTCGTCACTCACCGGTTGCATTACAAGTGGCAATGCAAGCGAT‑‑‑‑GCGTCAg                                                                                                                                      >  2:910261/1‑140 (MQ=255)
 tCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTAACAAGCGGCAAAGAAAGCGCT‑‑‑‑GCGTCAGt                                                                                                                                     >  1:760011/1‑140 (MQ=255)
   agcaagcaCAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCACTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCg                                                                                                                                   <  2:204708/140‑1 (MQ=255)
      aagcaCAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAg                                                                                                                                >  2:1765046/1‑140 (MQ=255)
          acaGCAGGCCGGGCTGGATAGCAACGTCTTCCCCAGCGAGTTAACCCTTGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAg                                                                                                                            <  2:678234/140‑1 (MQ=255)
           cagcagGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGt                                                                                                                           >  1:419233/1‑140 (MQ=255)
               agGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCt                                                                                                                       >  2:687638/1‑140 (MQ=255)
                ggCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCtt                                                                                                                      >  2:1911015/1‑140 (MQ=255)
                  ccGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTg                                                                                                                    <  2:1232714/140‑1 (MQ=255)
                  ccGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTg                                                                                                                    >  2:997616/1‑140 (MQ=255)
                  ccGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTg                                                                                                                    >  1:74721/1‑140 (MQ=255)
                   cGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGc                                                                                                                   >  1:1350858/1‑140 (MQ=255)
                   cGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGc                                                                                                                   >  1:35798/1‑140 (MQ=255)
                       cTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGc                                                                                                               <  2:419233/140‑1 (MQ=255)
                       cTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGc                                                                                                               >  1:952245/1‑140 (MQ=255)
                          gTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCgg                                                                                                            <  1:1831745/140‑1 (MQ=255)
                           ttAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGa                                                                                                           >  2:192651/1‑140 (MQ=255)
                            tAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGAc                                                                                                          <  1:687638/140‑1 (MQ=255)
                            tAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGAc                                                                                                          <  2:1478117/140‑1 (MQ=255)
                            tAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGAc                                                                                                          <  2:201637/140‑1 (MQ=255)
                            tAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGAc                                                                                                          <  2:1350858/140‑1 (MQ=255)
                            tAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGAc                                                                                                          <  1:192651/140‑1 (MQ=255)
                                    tCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAg                                                                                                  >  1:348839/1‑140 (MQ=255)
                                        ccccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGc                                                                                              >  2:715154/1‑140 (MQ=255)
                                          ccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                            <  1:1549869/140‑1 (MQ=255)
                                          ccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                            <  2:1448874/140‑1 (MQ=255)
                                          ccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                            <  1:1269978/140‑1 (MQ=255)
                                          ccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                            <  1:997616/140‑1 (MQ=255)
                                           cAGCGATTTAACCCTTGAATACCCCCTACCGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGAc                                                                                           <  1:837727/140‑1 (MQ=255)
                                           cAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGAc                                                                                           <  2:952245/140‑1 (MQ=255)
                                            cgCGTTTTAACCCTCCAATACGCCTTACAGCTGGAATCGAAAATGACCCGTCACTGGCCGCTAGCCTTACAAGAGGTAAAGCGGGCGGG‑‑‑‑GCGTCAGCCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCACAGGAGCcaca                                                                                          <  2:1894274/139‑1 (MQ=255)
                                            aGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGTAAGCCGGACTTGCCCAGGAGCGACa                                                                                          >  2:1714119/1‑140 (MQ=255)
                                            aGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACa                                                                                          <  2:1232664/140‑1 (MQ=255)
                                              cGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGt                                                                                        >  1:1175479/1‑140 (MQ=255)
                                                aTTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGc                                                                                              <  2:957330/132‑1 (MQ=255)
                                                aTTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGc                                                                                              >  1:957330/1‑132 (MQ=255)
                                                aTTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTa                                                                                      >  1:213410/1‑140 (MQ=255)
                                                aTTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTa                                                                                      <  1:1911015/140‑1 (MQ=255)
                                                  ttAACCCTCGAATACGCTTTACAGCTGGCATAGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTAtt                                                                                    <  2:672995/140‑1 (MQ=255)
                                                      cccTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCATGAAGTGGCTTTGAAAGCCGGACTTGGCCAGTAGCGACAGTTATTCAcc                                                                                >  2:691587/1‑140 (MQ=255)
                                                         tCGAATGCGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTg                                                                             <  2:302673/140‑1 (MQ=255)
                                                           gAATACGCCTTACAGTTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCt                                                                           <  1:651458/140‑1 (MQ=255)
                                                           gAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTCGCCGAGTAGCGACAGTTATTCACCTTGCt                                                                           >  1:661875/1‑140 (MQ=255)
                                                              tACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggc                                                                        <  1:1765046/140‑1 (MQ=255)
                                                              tACGCCTTACAGCTGACATCGACAACGGCGCGTCACTAGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggc                                                                        <  1:548223/140‑1 (MQ=255)
                                                                cGCCTTACGGCTGGCACCGAAATAGGCACGCAAGTTGCCTCTGGCCTTCCAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                      <  2:869922/140‑1 (MQ=255)
                                                                cGCCTTACAGCTGGCCTCGAAATTGCCACATCACTCGTCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGTGACAGTTATTCACCTTGCTggcgg                                                                      <  1:634314/140‑1 (MQ=255)
                                                                cGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTGCAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                      <  2:607189/140‑1 (MQ=255)
                                                                cGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGTAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                      <  1:1714119/140‑1 (MQ=255)
                                                                 gCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcggc                                                                     <  2:35798/140‑1 (MQ=255)
                                                                 gCCTTACAGCTGGCAGCGAAAACGGTACGTCACTCGCTGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcggc                                                                     <  1:872640/140‑1 (MQ=255)
                                                                   cTTACGGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCaa                                                                   <  2:433200/140‑1 (MQ=255)
                                                                   cTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTAtt                                                                                    >  2:1218060/1‑123 (MQ=255)
                                                                   cTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTAtt                                                                                    <  1:1218060/123‑1 (MQ=255)
                                                                     tACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggc                                                                        <  2:402859/133‑1 (MQ=255)
                                                                     tACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggc                                                                        >  1:402859/1‑133 (MQ=255)
                                                                      aCAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAACCGGACTTGCCCTGGAGCGACAGTTCTTCACCTTGCTGGCGGCAACag                                                                >  2:712703/1‑140 (MQ=255)
                                                                         gCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACagaag                                                             <  2:213410/140‑1 (MQ=255)
                                                                           tGGCATCGAAAATGGGACGTCAATCGCCGTTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGAt                                                           <  1:848873/140‑1 (MQ=255)
                                                                           tGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGGGCGACAGTTATTCATCTTGCTGGCGGCAACAGAAGAt                                                           >  1:926700/1‑140 (MQ=255)
                                                                           tGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGAt                                                           >  1:1311055/1‑140 (MQ=255)
                                                                           tGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGAt                                                           >  1:1689716/1‑140 (MQ=255)
                                                                            ggCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATc                                                          >  1:382691/1‑140 (MQ=255)
                                                                             gCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                      <  2:1495760/127‑1 (MQ=255)
                                                                             gCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                      >  1:1495760/1‑127 (MQ=255)
                                                                             gCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGTGGCAACAGAAGATCg                                                         >  1:703846/1‑140 (MQ=255)
                                                                             gCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCg                                                         >  2:446076/1‑140 (MQ=255)
                                                                                 cGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCAt                                                     <  1:715154/140‑1 (MQ=255)
                                                                                  gAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTCTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATg                                                    >  2:249202/1‑140 (MQ=255)
                                                                                    aaaTGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGaa                                                  <  2:198246/140‑1 (MQ=255)
                                                                                    aaaTGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGaa                                                  <  2:1462998/140‑1 (MQ=255)
                                                                                       tGGCACGTCACTCGCCGCTGGCCTTACAAGCCGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGc                                               <  2:760011/140‑1 (MQ=255)
                                                                                         gCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCAt                                             <  2:382691/140‑1 (MQ=255)
                                                                                         gCACGTCACTCGCCGATGGCCTCACTAGCGGCAAAGCGAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCAt                                             <  1:691587/140‑1 (MQ=255)
                                                                                             gTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTcc                                         >  2:461369/1‑140 (MQ=255)
                                                                                              tCACTCGCCGCTGGCCTCACAATCGGCAAAGGAAGTGCT‑‑‑‑TCGTCAGTCTCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCg                                        <  2:531905/140‑1 (MQ=255)
                                                                                                 cTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCtt                                     >  1:1040090/1‑140 (MQ=255)
                                                                                                    gccgcTGGCCTTACAAATGGCATAGCAAGCGTT‑‑‑‑TCGTCAGTCGCAGGATGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCt                                  <  2:926700/140‑1 (MQ=255)
                                                                                                         tGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAAGAGAAGATAGTCATGGAGGCATTTCCGCTTTCTTACaa                             >  1:531905/1‑140 (MQ=255)
                                                                                                                aCAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAacgca                      >  1:1774786/1‑140 (MQ=255)
                                                                                                                aCAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAacgca                      >  2:972363/1‑140 (MQ=255)
                                                                                                                    gCGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGcc                  <  2:348839/140‑1 (MQ=255)
                                                                                                                    gCGGCAAAGCAAGCGCT‑‑‑‑ACGTCTGTCGCAGGCAGTGGCTTTGCAAGCCAGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGcc                  <  2:491038/140‑1 (MQ=255)
                                                                                                                     cGGCAAAGCAAGCGCTACAGGCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAacgcac                     >  2:1402316/1‑140 (MQ=255)
                                                                                                                     cGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACCGAAGATCGGCATGAAGACATCTCCGATTTCTTACAAAAACGCACGccc                 >  2:808704/1‑140 (MQ=255)
                                                                                                                     cGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAATGCATCTCCGCTTTCTTACAAAAACGCACGccc                 <  1:473821/140‑1 (MQ=255)
                                                                                                                     cGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGccc                 >  2:1485779/1‑140 (MQ=255)
                                                                                                                     cGGCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGccc                 <  1:1008540/140‑1 (MQ=255)
                                                                                                                       gCAAAGCAAGCGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCATCTTGCTGGCGGCAACAGTAGATCGTCATGAGGGCTTCTCCGCTGTCTTACAAAAACGCACGCCCGa               >  2:598849/1‑140 (MQ=255)
                                                                                                                            gcaagcGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTa          <  1:446076/140‑1 (MQ=255)
                                                                                                                               agcGCT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAg       <  2:1567874/140‑1 (MQ=255)
                                                                                                                                gcgcT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAgg      >  1:375185/1‑140 (MQ=255)
                                                                                                                                gcgcT‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAgg      >  1:305204/1‑140 (MQ=255)
                                                                                                                                   ctacaggcgtCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAg       <  1:1402316/134‑1 (MQ=255)
                                                                                                                                    t‑‑‑‑GCGTCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGCCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGc  <  2:74721/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCT‑‑‑‑GCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGC  >  NC_000913/1457990‑1458261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑