Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	126,095	A→G	E134G (GAA→GGA)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	126,095	0	A	G	100.0%	291.8 / NA	81	E134G (GAA→GGA)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/48);  total (33/48)

AGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGT  >  NC_000913/125958‑126230                                                                                                                                          |                                                                                                                                        agaagagaagAAAGAAGCAGCTTCGACAGTAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGAc                                                                                                                                       <  1:923097/140‑1 (MQ=255) agaagagaagAAAGAAGCAGCTCCGGCAGCATCACTAGCCGCTGCGGCCGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGAc                                                                                                                                       <  2:770084/140‑1 (MQ=37) agaagagaagAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGCGAATGTTGGCGATCAAGTTGAAGCTGGAc                                                                                                                                       >  1:573899/1‑140 (MQ=255)    agagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATGCTGGTGAGAGTCGGGGATAAAGTTGAAGCTGGACAGt                                                                                                                                    >  1:696870/1‑140 (MQ=37)    agagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGt                                                                                                                                    >  2:980970/1‑140 (MQ=255)    agagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGt                                                                                                                                    >  1:248391/1‑140 (MQ=255)        aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCt                                                                                                                                <  2:1233791/140‑1 (MQ=255)        aagaaagaAGCAGCTCCGGCAGCAGCACCAGAGGCGGCGGCGGCAAAACACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCt                                                                                                                                <  2:696870/140‑1 (MQ=255)         agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTg                                                                                                                               >  2:1446486/1‑140 (MQ=255)         agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGAGAAAGTTGGCGATACAGTTGACGCTGGACAGTCGCTg                                                                                                                               >  1:706816/1‑140 (MQ=255)         agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTAACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTg                                                                                                                               >  1:1721208/1‑140 (MQ=255)           aaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGAt                                                                                                                             >  1:823930/1‑140 (MQ=255)             agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAg                                                                                                                                     >  2:1169064/1‑130 (MQ=255)             agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAg                                                                                                                                     <  1:1169064/130‑1 (MQ=255)             agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCt                                                                                                                                <  2:417019/135‑1 (MQ=255)             agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCt                                                                                                                                >  1:417019/1‑135 (MQ=255)                agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATATAGTTGAAGCTGGACAGTCGCTGATCACCg                                                                                                                        >  1:624101/1‑140 (MQ=255)                    gcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGa                                                                                                                    <  1:1237956/140‑1 (MQ=255)                     cTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGaa                                                                                                                   <  1:1270315/140‑1 (MQ=255)                         ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCg                                                                                                               >  1:1210839/1‑140 (MQ=255)                           cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                                             <  1:1855803/140‑1 (MQ=255)                           cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                                             >  2:1843169/1‑140 (MQ=255)                              cagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAg                                                                                                          >  2:1714661/1‑140 (MQ=255)                               agcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAgg                                                                                                         >  2:281493/1‑140 (MQ=255)                                gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGc                                                                                                        <  1:980970/140‑1 (MQ=255)                                gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGc                                                                                                        <  1:1446486/140‑1 (MQ=255)                                     aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa                                                                                                   >  1:1275611/1‑140 (MQ=255)                                           tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg                                                                                             <  1:1714661/140‑1 (MQ=255)                                                 ggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg                                                                                       <  2:1275611/140‑1 (MQ=255)                                                      aaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg                                                                                  <  1:974355/140‑1 (MQ=255)                                                              aaCGTTCCGGATATCCGCAGCGGCGTAGTAGAAGTGATCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg                                                                          <  1:641923/140‑1 (MQ=21)                                                                cGTTCCGGATATCGGCATCGACGAAGTTGAACTTATCGATATCCTGGTAAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCa                                                                        <  2:883816/140‑1 (MQ=21)                                                                cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCt                                                                            >  2:1243120/1‑136 (MQ=255)                                                                cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCt                                                                            <  1:1243120/136‑1 (MQ=255)                                                                cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCa                                                                        <  1:1395322/140‑1 (MQ=255)                                                                cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCa                                                                        <  2:1060179/140‑1 (MQ=255)                                                                cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCa                                                                        <  1:378912/140‑1 (MQ=255)                                                                   tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACGAGGGATCGATGGAGGTTCCGGGTCCGTTTGCTGGCACCg                                                                     >  2:557047/1‑140 (MQ=21)                                                                   tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg                                                                     >  1:50539/1‑140 (MQ=255)                                                                   tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg                                                                     <  2:1210839/140‑1 (MQ=255)                                                                      ggATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGa                                                                  >  2:114238/1‑140 (MQ=255)                                                                       gATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaa                                                                 <  2:49844/140‑1 (MQ=255)                                                                       gATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaa                                                                 <  2:1404472/140‑1 (MQ=255)                                                                            cGGCAGCGACGAAGTTGAAGTGGCCGAAATCCTGGCGAAGGTTGGCGACAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga                                                            <  2:624101/140‑1 (MQ=21)                                                                             ggCAGCGACGAAGTTTAAGTGAACGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                                           <  1:714091/140‑1 (MQ=255)                                                                             ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                                           <  1:1291053/140‑1 (MQ=255)                                                                             ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                                           <  2:823930/140‑1 (MQ=255)                                                                               cAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCa                                                         <  2:1734597/140‑1 (MQ=255)                                                                                 gCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa                                                       >  1:25658/1‑140 (MQ=255)                                                                                  cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAg                                                      >  1:1091114/1‑140 (MQ=255)                                                                                  cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTCCCGGCTCAGTTGGCAGGCACCATGAAAGAGATCAAAg                                                      >  1:620100/1‑140 (MQ=21)                                                                                         gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                               <  2:1519461/140‑1 (MQ=255)                                                                                         gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                               <  2:50539/140‑1 (MQ=255)                                                                                         gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                               <  1:114238/140‑1 (MQ=255)                                                                                         gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                               <  2:1081619/140‑1 (MQ=255)                                                                                                tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGAc                                        <  1:281493/140‑1 (MQ=255)                                                                                                 gACCGAAATCCTGGTTAAAGTTGGCAATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                       <  1:575741/140‑1 (MQ=255)                                                                                                 gACCGAAATCCTGGTGACAGTTTGCGATAAAGTTGGAGCTGGACAGTCTCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                       <  2:596394/140‑1 (MQ=37)                                                                                                 gACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                       <  2:283240/140‑1 (MQ=255)                                                                                                  aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTCGCACCGTAAAAGAGATCAAAGAGAACGTGGGTGACaa                                      >  1:744630/1‑140 (MQ=255)                                                                                                   ccGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCACCGAGAAAGAGAGCAAATTGAACGTGGGTGACaaa                                     >  2:609537/1‑140 (MQ=21)                                                                                                    cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                       <  1:1212805/137‑1 (MQ=255)                                                                                                    cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                       >  2:1212805/1‑137 (MQ=255)                                                                                                             ggTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCg                           <  1:968747/140‑1 (MQ=255)                                                                                                              gTGAAAGTTGGCGATTAAGTTGGAGATGGTCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                          <  1:667602/140‑1 (MQ=37)                                                                                                              gTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                          <  2:25658/140‑1 (MQ=255)                                                                                                              gTGAAAGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                          >  2:1481290/1‑140 (MQ=255)                                                                                                                 aaaGTTGGCGATAAAGTAGAAGATGGACAGTCGCTCATCCCCGTAGAACGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                       <  2:573899/140‑1 (MQ=37)                                                                                                                  aaGTTGGCGATAAAGTTTAAGCAGGACAGTCGCTGGTCACCGTAGAAGACGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCg                      <  2:776582/140‑1 (MQ=37)                                                                                                                  aaGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCg                      <  2:1091114/140‑1 (MQ=255)                                                                                                                  aaGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCg                      <  2:1721208/140‑1 (MQ=255)                                                                                                                  aaGTTGGCGATAAAGTTAAAGCTGGACAGTCGCTGATCAACGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCg                      <  2:706816/140‑1 (MQ=255)                                                                                                                   aGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                       >  1:1184733/1‑138 (MQ=255)                                                                                                                   aGTTGGCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                       <  2:1184733/138‑1 (MQ=255)                                                                                                                        gCGATAAAGTTGAAGCTGGACAGTCGCTGCTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAGGTGGACGGGGGTGACAGAGTGACTACTGGCTCggccgta                >  2:552163/1‑134 (MQ=21)                                                                                                                        gCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                          >  1:1760817/1‑130 (MQ=255)                                                                                                                        gCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                          <  2:1760817/130‑1 (MQ=255)                                                                                                                        gCGATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGGGGGTGACAGAGTGTCTACCGGCTCGCTGAtt                >  2:536118/1‑140 (MQ=255)                                                                                                                          gATAAAGTTGAAGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt              <  1:1843169/140‑1 (MQ=255)                                                                                                                                    aaGCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa    >  1:1455700/1‑140 (MQ=255)                                                                                                                                      gCTGGACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGt  >  2:1055020/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGT  >  NC_000913/125958‑126230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑